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  4. Recombinant Candida albicans pH-regulated antigen PRA1 (PRA1)

Recombinant Candida albicans pH-regulated antigen PRA1 (PRA1)

  • 中文名称：
    白念珠菌PRA1重组蛋白
  • 货号：
    CSB-EP308545CZD
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    白念珠菌PRA1重组蛋白
  • 货号：
    CSB-EP308545CZD-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    白念珠菌PRA1重组蛋白
  • 货号：
    CSB-BP308545CZD
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    白念珠菌PRA1重组蛋白
  • 货号：
    CSB-MP308545CZD
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Name:PRA1 Synonyms:FBP1 ORF Names:CaO19.10623, CaO19.3111
  • Uniprot No.：
  • 别名：
    PRA1; FBP1; CAALFM_C406980WA; CaO19.10623; CaO19.3111; pH-regulated antigen PRA1; 58 kDa fibrinogen-binding mannoprotein
  • 种属：
    Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    16-299
  • 氨基酸序列
    APVTV TRFVDASPTG YDWRADWVKG FPIDSSCNAT QYNQLSTGLQ EAQLLAEHAR DHTLRFGSKS PFFRKYFGND TASAEVVGHF ENVVGADKSS ILFLCDDLDD KCKNDGWAGY WRGSNHSDQT IICDLSFVTR RYLSQLCSGG YTVSKSKTNI FWAGDLLHRF WHLKSIGQLV IEHYADTYEE VLELAQENST YAVRNSNSLI YYALDVYAYD VTIPGEGCNG DGTSYKKSDF SSFEDSDSGS DSGASSTASS SHQHTDSNPS ATTDANSHCH THADGEVHC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cell surface protein involved in the host-parasite interaction during candidal infection. With MP65, represents a major component of the biofilm matrix. Sequesters zinc from host tissue and mediates leukocyte adhesion and migration. As a surface protein, binds the two human complement regulators CFH and CFHR1, as well as plasminogen PLG, mediates complement evasion and extra-cellular matrix interaction and/or degradation. As a released protein, enhances complement control in direct vicinity of the yeast and thus generates an additional protective layer which controls host complement attack, assisting the fungus in escaping host surveillance. Binds to host fluid-phase C3 and blocks cleavage of C3 to C3a and C3b, leading to inhibition of complement activation. Mediates also human complement control and complement evasion through binding to C4BPA, another human complement inhibitor, as well as through binding to host integrin alpha-M/beta-2. Decreases complement-mediated adhesion, as well as uptake of C.albicans by human macrophages.
  • 基因功能参考文献：
    1. Pra1 is a hierarchical complement inhibitor that targets C3 by cleaving C3 at a unique site. This inhibited effector function of the activation fragments. Pra1 also bound to C3a and C3b generated by human convertases and blocked their effector functions, C3a binding to human C3a receptor, calcium signaling, IL-8 release, C3b deposition, opsonophagocytosis, and killing by neutrophils. PMID: 28860090
    2. surface-exposed Pra1 plays a role in the recognition of Candida albicans, especially hyphal cells, by human neutrophils PMID: 21820180
    3. recognition of pH-regulated antigen 1 protein (Pra1p) by integrin alpha(M)betaplays a pivotal role in determining fungal virulence and host response and protection against C. albicans infection PMID: 21245270
    4. Data show that the fungus secretes a potent complement inhibitor, pH-regulated Ag 1 (Pra1), which in the direct surrounding of the pathogen binds to fluid-phase C3 and blocks cleavage of C3 to C3a and C3b. PMID: 20644161
    5. Therefore, it appears that Pra1p can play at most a minor role in fibrinogen binding to C. albicans. PMID: 18625733
    6. The pH-regulated antigen 1 (Pra1) protein was identified as a novel Factor H and FHL-1 binding protein. PMID: 19850343

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  • 亚细胞定位：
    Secreted. Note=Found primarily on the cell surface of filamentous forms and enriched at hyphal tips.
  • 蛋白家族：
    ZPS1 family
  • 数据库链接：

    KEGG: cal:CAALFM_C406980WA