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  4. Recombinant Bovine Guanylyl cyclase-activating protein 2 (GUCA1B)

Recombinant Bovine Guanylyl cyclase-activating protein 2 (GUCA1B)

  • 中文名称：
    牛GUCA1B重组蛋白
  • 货号：
    CSB-YP010045BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    牛GUCA1B重组蛋白
  • 货号：
    CSB-EP010045BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    牛GUCA1B重组蛋白
  • 货号：
    CSB-EP010045BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    牛GUCA1B重组蛋白
  • 货号：
    CSB-BP010045BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    牛GUCA1B重组蛋白
  • 货号：
    CSB-MP010045BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GUCA1B
  • Uniprot No.：
  • 别名：
    GUCA1B; GCAP-2; GCAP2Guanylyl cyclase-activating protein 2; GCAP 2; Guanylate cyclase activator 1B; Retinal guanylyl cyclase activator protein p24
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-204
  • 氨基酸序列
    GQQFSWEEA EENGAVGAAD AAQLQEWYKK FLEECPSGTL FMHEFKRFFK VPDNEEATQY VEAMFRAFDT NGDNTIDFLE YVAALNLVLR GTLEHKLKWT FKIYDKDRNG CIDRQELLDI VESIYKLKKA CSVEVEAEQQ GKLLTPEEVV DRIFLLVDEN GDGQLSLNEF VEGARRDKWV MKMLQMDLNP SSWISQQRRK SAMF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Stimulates two retinal guanylyl cyclases (GCs) GUCY2D and GUCY2F when free calcium ions concentration is low, and inhibits GUCY2D and GUCY2F when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GCs is a key event in recovery of the dark state of rod photoreceptors following light exposure. May be involved in cone photoreceptor response and recovery of response in bright light.
  • 基因功能参考文献：
    1. Mapping Calcium-Sensitive Regions in the Neuronal Calcium Sensor GCAP2 by Site-Specific Fluorescence Labeling. PMID: 27104297
    2. The C-terminal segment in GCAP2 confers target selectivity, facilitates membrane binding and provides sensitivity of the membrane localization of the protein to phosphorylation by rhodopsin kinase. PMID: 26001899
    3. Based on the distance constraints imposed by the cross-links, the study derived a 3D-structural model of the GCAP2 homodimer. PMID: 24026978
    4. Data suggest that dimerization domain of GUCY2D operates as a calcium-sensitive regulatory module; GUCY2D requires correct conformation of monomer-monomer interface for interaction with guanylate cyclase activating proteins (GCAP1; GCAP2). PMID: 23815670
    5. interaction between N-terminally myristoylated GCAP-2 and a peptide derived from the catalytic domain of full-length ROS-GC 1 PMID: 22631048
    6. Ca2+ exerted a stabilizing effect on both myristoylated and non-myristoylated forms of the protein, which was significantly stronger for myristoylated GCAP2. PMID: 21520322
    7. GCAP2 is a prime candidate for mediating Ca2+-dependent dynamic changes of synaptic ribbons in photoreceptor synapses PMID: 20463219
    8. Findings show that the sequence motif of the core GCAP2-modulatory site is Tyr965-Asn981 of rod outer segment membrane guanylate cyclase type 1 (ROS-GC1); this site is absolutely specific for GCAP2 and does not overlap with the GCAP1-modulated site. PMID: 15882072
    9. Our data indicate that the Ca(2+)-sensitivity of GCAP2 is significantly controlled by its third Ca(2+)-binding site, EF-hand 3. PMID: 17368568

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor. Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment.
  • 组织特异性：
    In the retina, expressed in rod and cone photoreceptor cells. Appears to be more abundant in rods.
  • 数据库链接：

    KEGG: bta:286851

    STRING: 9913.ENSBTAP00000035936

    UniGene: Bt.520