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  4. Recombinant Bovine Guanylyl cyclase-activating protein 1 (GUCA1A)

Recombinant Bovine Guanylyl cyclase-activating protein 1 (GUCA1A)

  • 中文名称：
    牛GUCA1A重组蛋白
  • 货号：
    CSB-YP010044BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    牛GUCA1A重组蛋白
  • 货号：
    CSB-EP010044BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    牛GUCA1A重组蛋白
  • 货号：
    CSB-EP010044BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    牛GUCA1A重组蛋白
  • 货号：
    CSB-BP010044BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    牛GUCA1A重组蛋白
  • 货号：
    CSB-MP010044BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GUCA1A
  • Uniprot No.：
  • 别名：
    GUCA1A; GCAP1; GUCA1; Guanylyl cyclase-activating protein 1; GCAP 1; Guanylate cyclase activator 1A
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-205
  • 氨基酸序列
    GNIMDGKSV EELSSTECHQ WYKKFMTECP SGQLTLYEFR QFFGLKNLSP WASQYVEQMF ETFDFNKDGY IDFMEYVAAL SLVLKGKVEQ KLRWYFKLYD VDGNGCIDRD ELLTIIRAIR AINPCSDSTM TAEEFTDTVF SKIDVNGDGE LSLEEFMEGV QKDQMLLDTL TRSLDLTRIV RRLQNGEQDE EGASGRETEA AEADG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Stimulates retinal guanylyl cyclase when free calcium ions concentration is low and inhibits guanylyl cyclase when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of retinal guanylyl cyclase is a key event in recovery of the dark state of rod photoreceptors following light exposure. May be involved in cone photoreceptor light response and recovery of response in bright light.
  • 基因功能参考文献：
    1. EPR double electron-electron resonance and site-directed mutagenesis analysis determined an atomic resolution structural model of a GCAP1 dimer. It has intermolecular hydrophobic contacts involving the side chain atoms of H19, Y22, F73 and V77. Mutating any of these at the dimer interface abolished dimerization. Dimerization may affect compartmentalization of GCAP1 in the photoreceptors and/or affect cyclase regulation. PMID: 29513743
    2. Data suggest that GCAP1 (Mg2+ vs. Ca2+) exhibits conformational changes in Ca2+ switch helix that are important in activation of RetGC1 (photoreceptor guanylate cyclase); myristoylation of GCAP1 is important as well in attaining activator conformation. PMID: 26703466
    3. Data suggest that dimerization domain of GUCY2D operates as a calcium-sensitive regulatory module; GUCY2D requires correct conformation of monomer-monomer interface for interaction with guanylate cyclase activating proteins (GCAP1; GCAP2). PMID: 23815670
    4. Presence of bound magnesium (Mg2+) in guanylate cyclase activator protein GCAP-1 is essential for its ability to stimulate retinal guanylyl cyclase. PMID: 16793776
  • 亚细胞定位：
    Membrane; Lipid-anchor. Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment.
  • 组织特异性：
    Detected in the retina. Detected in rod and cone photoreceptor cells (at protein level). Also present in certain pinealocytes.
  • 数据库链接：

    KEGG: bta:282243

    STRING: 9913.ENSBTAP00000017036

    UniGene: Bt.178