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  4. Recombinant Bacteroides fragilis Chaperone protein htpG (htpG), partial

Recombinant Bacteroides fragilis Chaperone protein htpG (htpG), partial

In Stock
  • 中文名称：
    Recombinant Bacteroides fragilis Chaperone protein htpG(htpG),partial
  • 货号：
    CSB-EP711569BAAB
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    htpG
  • Uniprot No.：
  • 别名：
    htpG; BF2491; Chaperone protein HtpG; Heat shock protein HtpG; High temperature protein G
  • 种属：
    Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    57.0 kDa
  • 表达区域：
    1-326aa
  • 氨基酸序列
    MQKGNIGVTTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLNTLASISEFKGELGDLTVHVSLGKDTITISDRGIGLTAEEIDKYINQIAFSGANDFLEKYKNDANAIIGHFGLGFYSAFMVSKKVEIITKSYKEGAQAVKWTCDGSPEFTLEEVEKADRGTDIVLYIDDDCKEFLEESRISALLKKYCSFLPVPIAFGKKKEWKDGKQVETAEDNVINDTIPLWTKKPSELSDEDYKKFYRELYPMSDEPLFWIHLNVDYPFHLTGILYFPKVKSNIDLNKNKIQLYCNQVYVTDSVEGIVPDFLTLLHGVLDSPDIPLNVSR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Recombinant B. fragilis htpG expression in E.coli requires the insertion of the target DNA fragment into an E.coli expression vector, routinely a plasmid vector, and the transferral of this vector into E.coli cells. The cells are then cultured and induced to express this htpG protein. The cells are harvested by centrifugation, samples prepared and proteins detected by polyacrylamide gel electrophoresis and subsequent staining of the gel with Coomassie Brilliant Blue or silver stain or by immunoblotting. The htpG protein expression in E.coli is highly scalable and can be adjusted from the bacterial colony to conical flasks for liquid cultures, to fermentation reaction chambers.

    ClpB and HtpG promote de novo protein folding in stressed E.coli cells. HtpG is a prokaryotic homolog of Hsp90 that stabilizes the bile body protein in the cyanobacterium Polychlorococcus elongatus PCC 7942. HtpG plays a role in cold acclimation in cyanobacteria. HtpG protein does not act as a cellular thermometer in Bacillus subtilis. The expression of htpG was found to be significantly up-regulated in P. plecoglossicida at its virulence temperature of 18°C.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Molecular chaperone. Has ATPase activity.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Heat shock protein 90 family
  • 数据库链接：

    KEGG: bfs:BF9343_2410

    STRING: 272559.BF2491