Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Bacillus subtilis Protein RecA (recA)

Recombinant Bacillus subtilis Protein RecA (recA)

  • 货号：
    CSB-YP325877BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP325877BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP325877BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP325877BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP325877BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    recA
  • Uniprot No.：
  • 别名：
    recA; recE; BSU16940; Protein RecA; Recombinase A
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-348
  • 氨基酸序列
    MSDRQAALDM ALKQIEKQFG KGSIMKLGEK TDTRISTVPS GSLALDTALG IGGYPRGRII EVYGPESSGK TTVALHAIAE VQQQGGQAAF IDAEHALDPV YAQKLGVNIE ELLLSQPDTG EQALEIAEAL VRSGAVDIVV VDSVAALVPK AEIEGDMGDS HVGLQARLMS QALRKLSGAI NKSKTIAIFI NQIREKVGVM FGNPETTPGG RALKFYSSVR LEVRRAEQLK QGNDVMGNKT KIKVVKNKVA PPFRTAEVDI MYGEGISKEG EIIDLGTELD IVQKSGSWYS YEEERLGQGR ENAKQFLKEN KDIMLMIQEQ IREHYGLDNN GVVQQQAEET QEELEFEE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multifunctional protein involved in homologous recombination, DNA repair and competence. Can catalyze the hydrolysis of (d)ATP in the presence of single-stranded DNA; prefers dATP at least in vitro, catalyzes the dATP-dependent uptake of single-stranded DNA by duplex DNA, and the dATP-dependent hybridization of homologous single-stranded DNAs (strand exchange). RecA-ATP cannot catalyze homologous DNA strand exchange; SsbA and DprA activate strand exchange by RecA-ATP. It interacts with LexA causing its activation and leading to its autocatalytic cleavage. Hydrolysis of ATP in the presence of single-stranded DNA is partially inhibited by RecU. Required for DNA transformation; protects transforming DNA from degradation, possibly in combination with DprA.; Recruited to repair centers (RCs), foci that are the site of double-stranded DNA break(s), after RecN. Concomitant with the appearance of RecO at the RCs, RecA forms threads that extend from RCs toward the opposite cell half, possibly searching for sequence homology along the sister chromosome. The threads disappear after about 2 hours. Thread formation is absolutely dependent on RecJ or AadAB.
  • 基因功能参考文献：
    1. s propose that repairing of the DNA lesions generates a replication stress to germinating spores, and the RecA.ssDNA filament might act by preventing potentially dangerous forms of DNA repair occurring during replication. PMID: 24285298
    2. RecX, by modulating the "length or packing" of a RecA filament, facilitates the initiation of recombination and increases recombination across species PMID: 23284295
    3. Results suggest that RecA, Nfo/ExoA, the NER system, and alpha/beta-type SASP all contribute to the repair of and/or protection against oxidative damage of DNA in germinating and outgrowing spores. PMID: 18203828
  • 亚细胞定位：
    Cytoplasm, nucleoid.
  • 蛋白家族：
    RecA family
  • 数据库链接：

    KEGG: bsu:BSU16940

    STRING: 224308.Bsubs1_010100009321