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  4. Recombinant Bacillus phage phi29 Histone-like protein p6 (6)

Recombinant Bacillus phage phi29 Histone-like protein p6 (6)

  • 货号：
    CSB-YP356118BBC
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356118BBC
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP356118BBC-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356118BBC
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356118BBC
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    6
  • Uniprot No.：
  • 别名：
    6; Histone-like protein p6; Double-stranded DNA-binding protein p6; Gene product 6; gp6; Nucleoid-associated protein p6; Protein p6
  • 种属：
    Bacillus phage phi29 (Bacteriophage phi-29)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-104
  • 氨基酸序列
    MAKMMQREIT KTTVNVAKMV MVDGEVQVEQ LPSETFVGNL TMEQAQWRMK RKYKGEPVQV VSVEPNTEVY ELPVEKFLEV ATVRVEKDED QEEQTEAPEE QVAE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Histone-like nucleoprotein that binds to the viral dsDNA and responsible for wrapping and compacting the viral DNA about 4-fold. Forms a nucleoprotein complex in which the DNA adopts a right-handed toroidal conformation winding around a protein core. Binds ito most, if not all, the viral genome, although with different affinity, the highest one corresponding to the genome ends. The formation of the nucleoprotein complex at the genome ends, activates the initiation of viral DNA replication. The binding of p6 would recruit the complex formed by the TP and the DNA polymerase to the origin. Protein p6 also represses early transcription from promoter C2, and, together with protein p4, represses transcription from promoters A2b and A2c and activates late transcription from promoter A3. Protein p6 is therefore involved in the early to late transcription switch. The formation of the nucleoprotein complex at the right end of the phage genome where the early promoter C2 is located affects local topology, which may contribute to the promoter repression.
  • 基因功能参考文献：
    1. the subcellular localization of protein p6 determined by immunofluorescence microscopy; at early infection stages, it localizes in a peripheral helix-like configuration; later, at middle infection stages, protein p6 is recruited to the bacterial nucleoid PMID: 22451942
  • 蛋白家族：
    Phi29likevirus histone-like protein p6 family
  • 数据库链接：

    KEGG: vg:6446509