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  4. Recombinant Arabidopsis thaliana Flavin-containing monooxygenase YUCCA1 (YUC1)

Recombinant Arabidopsis thaliana Flavin-containing monooxygenase YUCCA1 (YUC1)

  • 中文名称：
    拟南芥YUC1重组蛋白
  • 货号：
    CSB-YP886832DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥YUC1重组蛋白
  • 货号：
    CSB-EP886832DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥YUC1重组蛋白
  • 货号：
    CSB-EP886832DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥YUC1重组蛋白
  • 货号：
    CSB-BP886832DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥YUC1重组蛋白
  • 货号：
    CSB-MP886832DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    YUC1
  • Uniprot No.：
  • 别名：
    YUC1; YUC; YUCCA; YUCCA1; At4g32540; L23H3.20; Probable indole-3-pyruvate monooxygenase YUCCA1; EC 1.14.13.168; Flavin-containing monooxygenase YUCCA1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-414
  • 氨基酸序列
    MESHPHNKTD QTQHIILVHG PIIIGAGPSG LATSACLSSR GVPSLILERS DSIASLWKSK TYDRLRLHLP KHFCRLPLLD FPEYYPKYPS KNEFLAYLES YASHFRIAPR FNKNVQNAAY DSSSGFWRVK THDNTEYLSK WLIVATGENA DPYFPEIPGR KKFSGGKIVH ASEYKSGEEF RRQKVLVVGC GNSGMEISLD LVRHNASPHL VVRNTVHVLP REILGVSTFG VGMTLLKCLP LRLVDKFLLL MANLSFGNTD RLGLRRPKTG PLELKNVTGK SPVLDVGAMS LIRSGMIQIM EGVKEITKKG AKFMDGQEKD FDSIIFATGY KSNVPTWLQG GDFFTDDGMP KTPFPNGWRG GKGLYTVGFT RRGLLGTASD AVKIAGEIGD QWRDEIKGST RNMCSSRFVF TSKS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in auxin biosynthesis, but not in the tryptamine or the CYP79B2/B3 branches. Catalyzes in vitro the N-oxidation of tryptamine to form N-hydroxyl tryptamine. Involved during embryogenesis and seedling development. Required for the formation of floral organs and vascular tissues. Belongs to the set of redundant YUCCA genes probably responsible for auxin biosynthesis in shoots.
  • 基因功能参考文献：
    1. Data show that endoplasmic reticulum (ER) membrane anchored YUC proteins can mainly be found in roots, while cytosolic proteins are more abundant in the shoot. PMID: 28820425
    2. Studies indicate that YUCCA, a flavin-containing monooxygenase (YUC), catalyzes the last step of conversion from indole-3-pyruvate (IPyA) to indole-3-acetic acid (IAA). PMID: 26402640
    3. The induction of the YUCCA (YUC) genes increases Phenylacetic acid (PAA) metabolite levels in Arabidopsis, indicating that YUC flavin-containing monooxygenases may play a role in PAA biosynthesis. PMID: 26076971
    4. Yucasin is a potent inhibitor of YUC enzymes that offers an effective tool for analyzing the contribution of Indole-3-acetic acid (IAA) biosynthesis via the indole-3-pyruvic acid (IPyA) pathway to plant development and physiological processes. PMID: 24299123
    5. The analysis indicated that YUCCAs and TAA1, working in the IPA-YUC auxin biosynthesis pathway, are associated with SE induction, and that the expression of three YUCCA genes (YUC1, YUC4 and YUC10) is associated with LEC2 activity. PMID: 23722561
    6. YUCCAs (YUCs) encoding key enzymes in auxin biosynthesis are required for somatic embryo induction PMID: 23271028
    7. Data indicate that mature YUCCA 1 (YUC1) auxin biosynthesis gene overexpressing plants have elevated auxin levels and enhanced susceptibility to Pseudomonas syringae strain DC3000. PMID: 23521356
    8. YUCCA1 flavin monooxygenase functions in the indole-3-pyruvic acid branch of auxin biosynthesis PMID: 22108406
    9. Residues near the putative GXGXXG FAD binding site and the putative GXGXXG NADPH binding site are highly conserved in YUC flavin monooxygenases. PMID: 21205174
    10. These data demonstrate that auxin synthesized by the YUC flavin monooxygenases is an essential auxin source for Arabidopsis thaliana embryogenesis and postembryonic organ formation. PMID: 17704214

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  • 蛋白家族：
    FMO family
  • 组织特异性：
    Expressed in the apical meristems and young floral primordia. Detected in the floral meristems and at the base of the floral organs.
  • 数据库链接：

    KEGG: ath:AT4G32540

    STRING: 3702.AT4G32540.1

    UniGene: At.54581