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  4. Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor RAP2-2 (RAP2-2)

Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor RAP2-2 (RAP2-2)

  • 中文名称：
    拟南芥RAP2-2重组蛋白
  • 货号：
    CSB-YP885447DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥RAP2-2重组蛋白
  • 货号：
    CSB-EP885447DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥RAP2-2重组蛋白
  • 货号：
    CSB-EP885447DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥RAP2-2重组蛋白
  • 货号：
    CSB-BP885447DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥RAP2-2重组蛋白
  • 货号：
    CSB-MP885447DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAP2-2
  • Uniprot No.：
  • 别名：
    RAP2-2; ERF075; At3g14230; MLN21.1; Ethylene-responsive transcription factor RAP2-2; AtRAP2.2; Protein RELATED TO APETALA2 2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-379
  • 氨基酸序列
    MCGGAIISDF IPPPRSLRVT NEFIWPDLKN KVKASKKRSN KRSDFFDLDD DFEADFQGFK DDSAFDCEDD DDVFVNVKPF VFTATTKPVA SAFVSTGIYL VGSAYAKKTV ESAEQAEKSS KRKRKNQYRG IRQRPWGKWA AEIRDPRKGS REWLGTFDTA EEAARAYDAA ARRIRGTKAK VNFPEEKNPS VVSQKRPSAK TNNLQKSVAK PNKSVTLVQQ PTHLSQQYCN NSFDNSFGDM SFMEEKPQMY NNQFGLTNSF DAGGNNGYQY FSSDQGSNSF DCSEFGWSDH GPKTPEISSM LVNNNEASFV EETNAAKKLK PNSDESDDLM AYLDNALWDT PLEVEAMLGA DAGAVTQEEE NPVELWSLDE INFMLEGDF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor involved in carotenoid biosynthesis regulation. Binds to the 5'-ATCTA-3' element present in the promoter of phytoene synthase (PSY) and phytoene desaturase (PDS). Involved in ethylene response and resistance to necrotrophic pathogens. Acts as a downstream regulator in the ethylene signaling pathway. Partially redundant with RAP2-12.
  • 基因功能参考文献：
    1. ERF74 and ERF75 play a redundant role in the up-regulation of RbohD transcription and enhance the ROS burst during the early stages of the stress response. [ERF75] PMID: 28164334
    2. An evolutionarily conserved 12-bp cis-regulatory motif that binds to and is sufficient for activation by RAP2.2 and RAP2.12 is identified. PMID: 26668304
    3. RAP2.2, RAP2.3 and RAP2.12 play a role in modulating oxidative and osmotic stress tolerance during submergence acclimation. PMID: 25847219
    4. RAP2.2 functions as an important regulator in Botrytis resistance and ethylene responses. PMID: 22530619
    5. Ethylene and RAP2.2, together with oxygen-dependent signal transduction, play an important role in the response to hypoxia. PMID: 20357136
    6. The action of SINAT2 may explain the recalcitrance of AtRAP2.2 protein levels to change by altering AtRAP2.2 transcription. PMID: 17873090

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    AP2/ERF transcription factor family, ERF subfamily
  • 组织特异性：
    Constitutive in flowers, leaves, stems, and roots.
  • 数据库链接：

    KEGG: ath:AT3G14230

    STRING: 3702.AT3G14230.1

    UniGene: At.25146