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  4. Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 1 (DRB1)

Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 1 (DRB1)

  • 中文名称：
    拟南芥DRB1重组蛋白
  • 货号：
    CSB-YP520798DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥DRB1重组蛋白
  • 货号：
    CSB-EP520798DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥DRB1重组蛋白
  • 货号：
    CSB-EP520798DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥DRB1重组蛋白
  • 货号：
    CSB-BP520798DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥DRB1重组蛋白
  • 货号：
    CSB-MP520798DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DRB1
  • Uniprot No.：
  • 别名：
    DRB1; HYL1; At1g09700; F21M12.9Double-stranded RNA-binding protein 1; Protein HYPONASTIC LEAVES 1; dsRNA-binding protein 1; AtDRB1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-419
  • 氨基酸序列
    MTSTDVSSGV SNCYVFKSRL QEYAQKYKLP TPVYEIVKEG PSHKSLFQST VILDGVRYNS LPGFFNRKAA EQSAAEVALR ELAKSSELSQ CVSQPVHETG LCKNLLQEYA QKMNYAIPLY QCQKVETLGR VTQFTCTVEI GGIKYTGAAT RTKKDAEISA GRTALLAIQS DTKNNLANYN TQLTVLPCEK KTIQAAIPLK ETVKTLKARK AQFKKKAQKG KRTVAKNPED IIIPPQPTDH CQNDQSEKIE TTPNLEPSSC MNGLKEAAFG SVETEKIETT PNLEPPSCMN GLKEAAFGSV ETEKIETTPN LEPPSCMNGL KEAAFGSVET EKIETTPNLE PSSCMNGLKE AAFGSVETEK IETTPNLEPP SCMNGLKEAA FGSVETEKIE TTPNLESSSC MSGLKEAAFG SVETEASHA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Double-stranded RNA-binding protein involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis by assisting DICER-LIKE 1 (DCL1) in the accurate processing from primary miRNAs (pri-miRNAs) to miRNAs in the nucleus. Forms a complex with SERRATE (SE) and DCL1 to promote accurate processing of pri-miRNAs by DCL1. Binds and assist DCL1 for accurate processing of precursor miRNAs (pre-miRNA). Indirectly involved in the production of trans-acting small interfering RNAs (ta-siRNAs) derived from the TAS1, TAS2 or TAS3 endogenous transcripts by participating in the production of their initiating miRNAs. Involved with argonaute 1 (AGO1) in the guide strand selection from miRNA duplexes, presumably by directional loading of the miRNA duplex (guide stand and passenger strand) onto the RNA-induced silencing complex (RISC) for passenger strand degradation. Does not participate in sense transgene-induced post-transcriptional gene silencing (S-PTGS). Involved in several plant development aspects and response to hormones through its role in miRNAs processing.
  • 基因功能参考文献：
    1. HYL1 negatively regulate plant photomorphogenesis. PMID: 29522510
    2. High DRB1 expression is associated with defense against turnip crinkle virus. PMID: 29513740
    3. KETCH1 transports HYL1 from the cytoplasm to the nucleus. PMID: 28348234
    4. SMEK1, as a suppressor, inhibits MAPK activation to attenuate HYL1 phosphorylation; SMEK1 assembles a functional PP4 to target HYL1 for dephosphorylation PMID: 28586645
    5. Enzymes of the fatty acid degradation pathway were upregulated in DRB1-mutated plants. PMID: 26387911
    6. Data establishes that RNA binding by HYL1 dsRBD1 is essential for its function. PMID: 25409478
    7. The results suggest that HYL1 ensures the correct selection of pri-miRNA cleavage sites through homodimerization and thus contributes to gene silencing and plant development. PMID: 25294831
    8. The C-terminal dsRBDs of DCL1 and N-terminal dsRBDs of HYL1 have parallel roles. PMID: 23886622
    9. The role of HYL1 is in establishment of stamen architecture provides insight into the molecular mechanism of male fertility. PMID: 23918970
    10. HYL1 controls the miR156-mediated juvenile phase of vegetative growth. PMID: 22268150
    11. HYL1 monitors the roles of miR165/166, miR319a, and miR160 in leaf flattening through the relative activities of adaxial and abaxial identity genes. PMID: 21610018
    12. HYL1 has a function in assisting efficient and precise cleavage of pri-miRNA through interaction with DCL1 PMID: 16428603
    13. The N-terminal double-stranded RNA binding domains fulfill the function of the whole HYL1 and thus play an essential role in miRNA processing and miRNA-directed silencing of targeted genes. PMID: 17337628
    14. the distinct HYL1- and DCL1-containing nuclear bodies may be miRNA precursor processing sites or assembly and storage sites for the miRNA precursor processing machinery PMID: 17369351
    15. SINE RNA interacts with HYL1 that associates with the Dicer homologue DCL1 to produce microRNAs. PMID: 18551175
    16. Genetic screening identified a novel semidominant mutation in DCL1 (dcl1-13), this mutation confers significant restoration from the developmental abnormality and a reduction in the level of miRNA in the loss-of-function mutant of HYL1. PMID: 19155326
    17. In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced pri-miRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs. PMID: 19304749
    18. suggests that small RNA processing in the DCL1/DRB1-directed miRNA and DCL4/DRB4-directed sRNA biogenesis pathways operates via different mechanisms. PMID: 19861421

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  • 亚细胞定位：
    Nucleus. Nucleus speckle. Note=Localizes to nuclear dicing body (also named D body), a nuclear body distributed throughout the nucleoplasm and involved in miRNA processing.
  • 组织特异性：
    Expressed in rosette and cauline leaves, stems, roots, flowers and siliques.
  • 数据库链接：

    KEGG: ath:AT1G09700

    STRING: 3702.AT1G09700.1

    UniGene: At.10223