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ygcB Antibody

  • 中文名称：
    ygcB兔多克隆抗体
  • 货号：
    CSB-PA334357XA01ENV
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in Recombinant protein
      All lanes: ygcB antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 31.8 kDa
      Observed band size: 32 kDa
  • 其他：

产品详情

  • Uniprot No.：
    P38036
  • 基因名：
    ygcB
  • 别名：
    CRISPR-associated endonuclease/helicase Cas3 (EC 3.1.-.-) (EC 3.6.4.-) ygcB cas3 b2761 JW2731
  • 反应种属：
    Escherichia coli (strain K12 / DH10B)
  • 免疫原：
    Recombinant Escherichia coli (strain K12) CAS3 protein
  • 免疫原种属：
    Escherichia coli (strain K12)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Protein G
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity.; Acts as an endonuclease, a 3'-5'exonuclease, and an ATP-dependent dsDNA helicase. Anneals and unwinds R-loops (in which crRNA binds the target DNA, displacing the noncomplementary strand). Unwinding requires ATP, annealing does not. Required along with the Cascade complex for resistance to bacteriophage lambda infection as well as the ability to cure CRISPR-encoding high-copy number plasmid. A Cas3-CasA fusion protein purified with the Cascade complex nicks target plasmid in the presence but not absence of Mg(2+), and degrades plasmid fully in the presence of Mg(2+) and ATP, suggesting the helicase activity is required for complete degradation.
  • 基因功能参考文献：
    1. Cas3-derived target DNA degradation fragments fuel primed CRISPR adaptation. PMID: 27546790
    2. study observed that Cas3 promoted runaway ColE1 plasmid replication, requiring Cas3 helicase activity; Cas3 expression antagonized RNaseHI, observed as a concatamer phenotype, but also required RNaseHI for its ability to stimulate plasmid copy number PMID: 23406879
    3. Cas3 is involved in DNA degradation and invader neutralization. PMID: 22521689
    4. Study discuss possible overlapping roles of Cas3 and Cascade in E. coli, and the apparently antagonistic roles of Cas3 catalysing RNA-DNA annealing and ATP-dependent helicase unwinding. PMID: 21699496
  • 蛋白家族：
    CRISPR-associated nuclease Cas3-HD family; CRISPR-associated helicase Cas3 family
  • 数据库链接：

    KEGG: ecj:JW2731

    STRING: 316385.ECDH10B_2929