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VPS26B Antibody

  • 中文名称：
    VPS26B兔多克隆抗体
  • 货号：
    CSB-PA676824LA01HU
  • 规格：
    ￥3900
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MCF7 whole cell lysate, A431 whole cell lysate
      All lanes: VPS26B antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 39 kDa
      Observed band size: 39 kDa
    • IHC image of CSB-PA676824LA01HU diluted at 1:50 and staining in paraffin-embedded mouse brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
    • Immunofluorescence staining of Hela cell with CSB-PA676824LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) VPS26B Polyclonal antibody
  • Uniprot No.：
    Q4G0F5
  • 基因名：
  • 别名：
    Pep8b antibody; Vacuolar protein sorting 26 homolog B (S. pombe) antibody; Vacuolar protein sorting 26 homolog B (yeast) antibody; Vacuolar protein sorting-associated protein 26B antibody; Vesicle protein sorting 26B antibody; VP26B_HUMAN antibody; VPS26B antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Vacuolar protein sorting-associated protein 26B protein (1-336AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，VPS26B Antibody (CSB-PA676824LA01HU)，的标记方式是Non-conjugated。对于VPS26B Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA676824LB01HU VPS26B Antibody, HRP conjugated ELISA
    FITC CSB-PA676824LC01HU VPS26B Antibody, FITC conjugated
    Biotin CSB-PA676824LD01HU VPS26B Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.02% sodium azide
    Constituents: PBS containing 50% glycerol pH 7.3
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC  1:20-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. May be involved in retrograde transport of SORT1 but not of IGF2R. Acts redundantly with VSP26A in SNX-27 mediated endocytic recycling of SLC2A1/GLUT1.
  • 基因功能参考文献：
    1. s propose that PAR-2 plasma membrane repopulation is regulated by Vps26B-retromer, describing a potential novel role for this complex. PMID: 26113136
    2. Colocalization of Vps26 paralogues with different endosomally located Rab proteins shows prolonged association of Vps26B-retromer with maturing endosomes relative to Vps26A-retromer. PMID: 21920005
  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein. Early endosome. Late endosome.
  • 蛋白家族：
    VPS26 family
  • 数据库链接：

    HGNC: 28119

    OMIM: 610027

    KEGG: hsa:112936

    STRING: 9606.ENSP00000281187

    UniGene: Hs.334684