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TOP3A Antibody

  • 中文名称：
    TOP3A兔多克隆抗体
  • 货号：
    CSB-PA698044
  • 规格：
    ￥880
  • 图片：
    • Western blot analysis of 293T using Topo IIIα antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
  • 其他：

产品详情

  • Uniprot No.：
    Q13472
  • 基因名：
    TOP3A
  • 别名：
    DNA topoisomerase 3 alpha antibody; DNA topoisomerase 3-alpha antibody; DNA topoisomerase III alpha antibody; TOP 3 antibody; TOP3 antibody; TOP3A antibody; TOP3A_HUMAN antibody; topo III-alpha antibody; topoisomerase (DNA) III alpha antibody; topoisomerase (DNA) III antibody; topoisomerase III alpha antibody; ZGRF7 antibody; zinc finger; GRF-type containing 7 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human Topo IIIα. at AA rangle: 350-430
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2.
  • 基因功能参考文献：
    1. Top3alpha is shown to be an essential component of the mitochondrial DNA replication machinery and as the first component of the mitochondrial DNA separation machinery. PMID: 29290614
    2. The results show that Topo IIIalpha stimulates DNA unwinding by BLM in a manner that is potentiated by RMI1-RMI2, and that the processivity of resection is reliant on the Topo IIIalpha-RMI1-RMI2 complex. PMID: 25200081
    3. hTOP3alpha interacts with p53, regulates p53 and p21 expression, and contributes to the p53-mediated tumor suppression PMID: 24526736
    4. MPS1 protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III alpha-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis PMID: 24108125
    5. Study found a correlation between topoisomerase IIIalpha immunoexpression and oral squamous cell carcinoma (OSCC) cancer stem cell immunophenotypes. However, results did not show a prognostic significance of topoisomerases in OSCC> PMID: 22672768
    6. TOP3A, a new partner gene fused to MLL in an adult patient with de novo acute myeloid leukaemia. PMID: 22050635
    7. human topoisomerase IIIalpha functions as a decatenase with the assistance of BLM and RMI1 to facilitate the processing of homologous recombination intermediates without crossing over as a mechanism to preserve genome integrity PMID: 20445207
    8. G-quadruplex ligand telomestatin impairs binding of topoisomerase IIIalpha to G-quadruplex-forming oligonucleotides and uncaps telomeres in ALT cells PMID: 19742304
    9. Dual localization to mitochondria and nucleus PMID: 12209014
    10. The Bloom's syndrome helicase stimulates the activity of topoisomerase IIIalpha PMID: 12433984
    11. The effects of TOP3 on telomere binding in human and S. cerevisiae cells are reported. PMID: 16546998
    12. BLM, Topo IIIalpha, and BLAP75 constitute a dissolvasome complex that processes HR intermediates to limit DNA crossover formation PMID: 16595695
    13. BLM is stably associated with TOP3A. PMID: 17728255
    14. evolutionarily conserved N-terminal third of BLAP75 mediates complex formation with BLM and Topo IIIalpha and that the DNA binding activity resides in the C-terminal third of this novel protein. PMID: 18390547
    15. Topo IIIalpha is an important telomere-associated factor, essential for telomere maintenance and chromosome stability in ALT cells, and speculate on its potential mechanistic function. PMID: 18418389
    16. Individuals carrying genetic variants of the BLM-TOP3A-RMI1 complex have an increased risk of acute myeloid leukemia/myelodysplatic syndromes, malignant melanoma, bladder and breast cancer. PMID: 19432957

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  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    Type IA topoisomerase family
  • 组织特异性：
    High expression is found in testis, heart, skeletal muscle and pancreas.
  • 数据库链接：

    HGNC: 11992

    OMIM: 601243

    KEGG: hsa:7156

    STRING: 9606.ENSP00000321636

    UniGene: Hs.592115