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  4. RFC1 Antibody

RFC1 Antibody

  • 中文名称：
    RFC1兔多克隆抗体
  • 货号：
    CSB-PA005450
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of mouse liver mouse kidney cells using RFC1 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    P35251
  • 基因名：
  • 别名：
    A1 140 kDa subunit antibody; A1 antibody; A1 P145 Activator 1 large subunit antibody; Activator 1 140 kDa subunit antibody; Activator 1 large subunit antibody; Activator 1 subunit 1 antibody; DNA binding Protein PO GA antibody; DNA-binding protein PO-GA antibody; MHC binding factor beta antibody; MHCBFB antibody; PO GA antibody; RECC1 antibody; Replication factor C (activator 1) 1, 145kDa antibody; Replication factor C 140 kDa subunit antibody; Replication factor C antibody; Replication factor C large subunit antibody; Replication factor C subunit 1 antibody; Replication factor C1 antibody; RF-C 140 kDa subunit antibody; RFC antibody; RFC1 antibody; RFC1_HUMAN antibody; RFC140 antibody; RFC140 Replication Factor C 140 kDa subunit antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human RFC1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.; Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
  • 基因功能参考文献：
    1. Human CTF18-RFC clamp-loader complexed with non-synthesising POLE efficiently loads the PCNA sliding clamp. PMID: 28199690
    2. RFC depletion had a negative impact on LANA's ability to replicate and maintain viral DNA in cells containing artificial KSHV episomes or in infected cells, leading to loss of virus. PMID: 25071216
    3. phosphorylation of serine 51 on hLigI plays a critical role in regulating the interaction between hLigI and RFC, which is required for efficient DNA replication and repair. PMID: 22952233
    4. report that the large subunit (RFC1) of replication factor C is cleaved in Hutchinson-Gilford progeria syndrome cells, leading to the production of a truncated RFC1 of ~ 75 kDa PMID: 22168243
    5. We identified a variant in each of 3 genes (RFC1, SCN1A, ANPEP) potentially implicated in susceptibility to severe neurological complications in West Nile Virus disease. PMID: 21881118
    6. Small interfering RNA-mediated knockdown of RFC1 impairs upstream removal of UV lesions and abrogates the downstream recruitment of DNA polymerase delta. PMID: 20713449
    7. analysis of the DNA-bound BRCA1 C-terminal region structure from human replication factor C p140 and model of the protein-DNA complex PMID: 20081198
    8. interaction with histone deacetylase HDAC1 PMID: 12045192
    9. RFC (p140) has an important role as a regulator of transcription and NF-kappaB activity PMID: 12509469
    10. destabilization of the RF-C complex by CDKs may inactivate the RF-C complex at the end of S phase PMID: 12947101
    11. NMR resonance assignments of the BRCT region of the large subunit PMID: 15772763
    12. Ctf18-RFC interacts physically with pol eta, which allows DNA replication forks to overcome interference by various template structures, including damaged DNA and DNA-protein complexes that maintain chromosome cohesion PMID: 17545166
    13. Data show that replication factor C (RFC), via several stimulatory motifs per molecule, potently activates flap endonuclease 1 (FEN1), making RFC a critical partner with FEN1 for the processing of eukaryotic Okazaki fragments. PMID: 19208620
    14. a mutant version of hLigI, which mimics the hyperphosphorylated M-phase form of hLigI, does not interact with and is not inhibited by RFC, demonstrating that inhibition of ligation is dependent upon the interaction between hLigI and RFC PMID: 19223468

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Activator 1 large subunit family
  • 组织特异性：
    Wide tissue distribution. Undetectable in placental tissue.
  • 数据库链接：

    HGNC: 9969

    OMIM: 102579

    KEGG: hsa:5981

    STRING: 9606.ENSP00000371321

    UniGene: Hs.507475