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PUS10 Antibody

  • 中文名称：
    PUS10兔多克隆抗体
  • 货号：
    CSB-PA484448
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA484448(PUS10 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA484448(PUS10 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse intestinum tenue tissue, Primary antibody: CSB-PA484448(PUS10 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds
  • 其他：

产品详情

  • Uniprot No.：
    Q3MIT2
  • 基因名：
    PUS10
  • 别名：
    CCDC139 antibody; Coiled coil domain containing 139 antibody; Coiled coil domain containing protein 139 antibody; Coiled-coil domain-containing protein 139 antibody; DOBI antibody; FLJ32312 antibody; MGC126729 antibody; Pseudouridine synthase 10 antibody; Pseudouridylate synthase 10 antibody; Psi55 synthase antibody; pus10 antibody; PUS10_HUMAN antibody; Putative tRNA pseudouridine synthase Pus10 antibody; tRNA pseudouridine 55 synthase antibody; tRNA pseudouridylate synthase antibody; tRNA uridine isomerase antibody; tRNA-uridine isomerase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthetic peptide of Human PUS10
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    WB 1:200-1:1000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
  • 基因功能参考文献：
    1. The caspase-3-mediated movement of PUS10 and the release of mitochondrial contents enhancing caspase-3 activity creates a feedback amplification loop for caspase-3 action. Therefore, any defect in the movement or interactions of PUS10 would reduce the TRAIL sensitivity of tumor cells. PMID: 28981101
    2. crystal structure of Pus10 (previously denoted as FLJ32312) at 2.0 A resolution and modeling studies describe protein domains and active sites PMID: 17900615
    3. These data suggest that the cleaved DOBI may acquire a new function, possibly by cooperating with tBid in the mitochondrial event of cell death caused by TRAIL. PMID: 19712588
  • 亚细胞定位：
    Nucleus. Cytoplasm. Mitochondrion.
  • 蛋白家族：
    Pseudouridine synthase Pus10 family
  • 数据库链接：

    HGNC: 26505

    OMIM: 612787

    KEGG: hsa:150962

    STRING: 9606.ENSP00000326003

    UniGene: Hs.368348