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DNAJB1 Antibody

  • 中文名称：
    DNAJB1兔多克隆抗体
  • 货号：
    CSB-PA288820
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from COLO205 cells, using HSP40 antibody.
    • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HSP40 antibody.
    • Immunofluorescence analysis of NIH/3T3 cells, using HSP40 antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) DNAJB1 Polyclonal antibody
  • Uniprot No.：
    P25685
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human HSP40.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
    IF 1:100-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).
  • 基因功能参考文献：
    1. this study shows that DNAJB1/HSP40 suppresses Melanoma Differentiation-Associated Gene 5-Mitochondrial Antiviral Signaling Protein function in conjunction with HSP70 PMID: 29069650
    2. Modeling of the different conformations of PRKACA-DNAJB1 Chimeric Kinase revealed no obvious steric interactions of the J-domain with the rest of the RIIbeta holoenzyme. PMID: 29335433
    3. Data show that DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1) is a transcriptional target of forkhead box protein E3 (FOXE3) in a pathway that is crucial for the development of the anterior segment of the eye. PMID: 27218149
    4. These results indicate that Mixed fibrolamellar hepatocellular carcinoma (mFL-HCC) is similar to pure FL-HCC at the genomic level and the DNAJB1:PRKACA fusion can be used as a diagnostic tool for both pure and mFL-HCC PMID: 27029710
    5. These studies demonstrate an important role for cellular chaperone Hsp40/DnaJB1 in influenza A virus life cycle by assisting nuclear trafficking of viral ribonucleoproteins. PMID: 26750153
    6. DNAJB1-PRKACA was evaluated as a key driver of fibrolamellar carcinoma and as a candidate therapeutic target PMID: 26505878
    7. study provides a new molecular mechanism to regulate EGFR signaling through modulation of MIG6 by DNAJB1 as a negative regulator. PMID: 26239118
    8. Whole exome sequencing followed by immunohistochemistry of fibrolamellar hepatocellular carcinoma cell lines and tumors showed two structural variants resulting in fusion transcripts: DNAJB1-PRKCA and CLPTM1L-GLIS3. PMID: 25122662
    9. In this cohort of fibrolamellar hepatocellular carcinoma, almost 80% contained the DNAJB1-PRKACA fusion transcript. PMID: 25557953
    10. s identified DNAJB1 as a negative regulator of PDCD5-mediated apoptosis and found that the apoptosis network of PDCD5 regulates cancer cell death. PMID: 25444898
    11. We also found that, despite an initial report to the contrary, the human homolog of Sis1, Hdj1, is capable of [PSI+] prion propagation in place of Sis1 PMID: 25058638
    12. HSP40 interacts with pyruvate kinase M2 and regulates glycolysis and cell proliferation in tumor cells. PMID: 24658033
    13. Studies with specific phosphoantibodies indicate that MK5 phosphorylates Hsp40/DnaJB1 in vivo at Ser-149 or/and Ser-151 and Ser-171 in the C-terminal domain of Hsp40/DnaJB1. PMID: 24309468
    14. Evidence supporting the presence of the DNAJB1-PRKACA chimeric transcript in 100% of the FL-HCCs examined (15/15) suggests that this genetic alteration contributes to tumor pathogenesis. PMID: 24578576
    15. we discovered a new MDM2 interacting protein, DNAJB1, and provided evidence to support its p53-dependent tumor suppressor function. PMID: 24361594
    16. HSP40 and HSP110 function together in protein homeostasis control. PMID: 24091676
    17. methionine deprivation results in an antioxidant response that includes an increase in the levels of HSPA1A and DNAJB1 mRNA PMID: 23395854
    18. There was a positive correlation between DnaJB1 and severity of pulmonary arterial hypertension in peripheral blood mononuclear cells from patients with limited cutaneous systemic sclerosis. PMID: 23400395
    19. Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway. PMID: 22075554
    20. Mammalian Hsp110 (Apg-2), Hsp70 (Hsc70 or Hsp70) and Hsp40 (Hdj1) were necessary and sufficient to slowly dissolve large disordered aggregates and recover natively folded protein PMID: 22022600
    21. Cellular localization studies showed that NP and Hsp40 co-localize primarily in the nucleus. During IAV infection in mammalian cells, expression of NP coincided with the dissociation of P58(IPK) from Hsp40 and decrease PKR phosphorylation PMID: 21698289
    22. The antiviral activity of overexpressed DNAJC14 occurs in a time- and dose-dependent manner. PMID: 21249176
    23. The crystal structure of full-length Hdj1 was diffracted to 3.90A resolution. PMID: 21139202
    24. 5-fluorouracil & carboplatin specifically induce expression of Hsp40 in hepatoma cells. siRNA knockdown of Hsp40 diminishes survival of drug-exposed cells. PMID: 19901540
    25. Altered expression of HSPF1 was found in brains and lymphoblastoid cells from patients with bipolar disorder. PMID: 14743183
    26. sequestered in discrete foci in the nucleus of the infected cell in herpes simplex virus type 1 infection PMID: 15194794
    27. It is confirmed an increased expression of HSPF1 in the lymphoblastoid cell lines from patients with bipolar I disorder, bipolar II disorder, and schizophrenia. PMID: 15362566
    28. Hsp40 is crucial for Nef-mediated enhancement of viral gene expression and replication PMID: 16179353
    29. hepatitis B virus X protein is the major target of Hdj1 in the inhibition of hepatitis B virus replication PMID: 16842747
    30. These results demonstrate a role for Hsp40/DnaJB6 in the regulation of HIV-2 PIC nuclear transport. PMID: 18032501
    31. The 2.7A structure reveals that Hdj1 forms a homodimer in the crystal by a crystallographic two-fold axis. PMID: 18211704

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
  • 数据库链接：

    HGNC: 5270

    OMIM: 604572

    KEGG: hsa:3337

    STRING: 9606.ENSP00000254322

    UniGene: Hs.515210