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  4. Bovine Pregnancy Specific Protein B(PSPB)ELISA Kit

Bovine Pregnancy Specific Protein B(PSPB)ELISA Kit

  • 中文名称：
    牛妊娠特异性蛋白B（PSPB）酶联免疫试剂盒
  • 货号：
    CSB-E13353B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine PAG1 ELISA Kit was designed for the quantitative measurement of Bovine PAG1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.06 ng/mL-24 ng/mL and the sensitivity is 0.06 ng/mL.

  • 别名：
    Pregnancy-associated glycoprotein 1 ELISA Kit; PAG 1 ELISA Kit; EC 3.4.23.- ELISA Kit; Pregnancy-specific protein B ELISA Kit; PSP-B ELISA Kit
  • 缩写：
    PAG1
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.06 ng/mL-24 ng/mL
  • 灵敏度：
    0.06 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PSPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-104  
    1:2 Average % 94  
    Range % 88-105  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 97  
    Range % 89-105  
  • 回收率：
    The recovery of bovine PSPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 84-94  
    EDTA plasma (n=4) 97 90-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    24 0.155 0.146 0.151    
    4.5 0.344 0.332 0.338    
    0.75 0.571 0.582 0.577    
    0.21 1.006 0.998 1.002    
    0.06 1.562 1.575 1.569    
    0 2.191 2.223 2.207    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Appears to be proteolytically inactive.
  • 基因功能参考文献：
    1. This study examines expression patterns of the genes SERPINA14, PAG, and PAG2 at the fetal-maternal interface in dairy heifers experimentally infected with N caninum during the second trimester of pregnancy, when most abortions takes place in natural conditions. PMID: 27045629
    2. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH. PMID: 26936628
    3. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
    4. Restraint stress for 2 h during early pregnancy in heifers increased blood cortisol, but it did not affect the concentrations of progesterone and PAG-1 between days 30 to 40 of gestation. PMID: 21872319
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase A1 family
  • 组织特异性：
    Trophoblast and placental tissue. Produced specifically in the invasive binucleate cells of the placenta. Becomes detectable in maternal serum soon after implantation.
  • 数据库链接：