Akt2

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Rat liver tissue
All lanes: AKT2 antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56, 52 kDa
Observed band size: 56 kDa

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA15919A0Rb at dilution of 1:100

Immunofluorescence staining of A549 cells with CSB-PA15919A0Rb at 1:466, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).