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中文名称:大鼠8羟基脱氧鸟苷(8-OHdG)酶联免疫试剂盒
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货号:CSB-E10526r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:8-OHdG,也称为 8-羟基脱氧鸟苷,是体内氧化应激的标志物。它是一种生物标志物,可用于评估氧化损伤的水平和识别潜在的健康问题。 8-OHdG 已被发现在患有某些疾病(如癌症、糖尿病、心血管疾病和神经退行性疾病)的个体中升高。此外,它还与衰老和空气污染等环境压力因素有关。研究表明,测量 8-OHdG 水平可以帮助诊断疾病状态和监测治疗效果。因此,它是临床医学中评估患者氧化应激的重要工具。j9九游会登录入口首页生物所提供的Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆样本中的8-OHdG,其灵敏度为j9九游会登录入口首页生物所提供的0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
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缩写:8-OHdG
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, cell culture supernates, tissue homogenates
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检测范围:0.312 ng/mL-20 ng/mL
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灵敏度:0.078 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cell Biology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 88-98 1:2 Average % 96 Range % 91-102 1:4 Average % 90 Range % 85-96 1:8 Average % 96 Range % 88-99 -
回收率:
The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 102 98-107 EDTA plasma (n=4) 96 90-102 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 1.890 1.780 1.835 1.682 10 1.198 1.128 1.163 1.010 5 0.756 0.726 0.741 0.588 2.5 0.525 0.495 0.510 0.357 1.25 0.321 0.318 0.320 0.167 0.625 0.272 0.262 0.267 0.114 0.312 0.213 0.209 0.211 0.058 0 0.154 0.152 0.153 -
数据处理:
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货期:3-5 working days
引用文献
- Diamond-Like Carbon Depositing on the Surface of Polylactide Membrane for Prevention of Adhesion Formation During Tendon Repair Y Xiao,Nano-micro letters,2024
- Edible wild plants, chicory and purslane, alleviated diabetic testicular dysfunction, and insulin resistance via suppression 8OHdg and oxidative stress in rats EA Saad,PloS one,2024
- Mechanistic aspects of ameliorative effects of Eicosapentanoic acid ethyl ester on methotrexate-evoked testiculopathy in rats NAT Abbas,NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY,2024
- Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain WJ Song,Toxics,2023
- Anti-radiation effect of MRN-100: a hydro-ferrate fluid, in vivo Y Xue,Journal of radiation research,2024
- Gastroprotective effect of dapagliflozin in ethanol-induced gastric lesions in rats: Crosstalk between HMGB1/RAGE/PTX3 and TLR4/MyD88/VEGF/PDGF signaling pathways RM Salama,International immunopharmacology,2023
- Neuroprotective effect of peanut against oxidative stress in streptozotocin-induced diabetic rats NH Mohamed,Egyptian Journal of Basic and Applied Sciences,2022
- Hydrogen exerts neuroprotective effects by inhibiting oxidative stress in experimental diabetic peripheral neuropathy rats XC Han,Medical Gas Research,2022
- The Effect of Metformin in Diabetic and Non-Diabetic Rats with Experimentally-Induced Chronic Kidney Disease MA Za'abi,Biomolecules,2021
- Graphene oxide induces dose‑dependent lung injury in rats by regulating autophagy Mingke Qiu,Experimental and Therapeutic Medicine,2021
- β-Sitosterol attenuates liver injury in a rat model of chronic alcohol intake Z Chen,Archives of Pharmacal Research,2020
- Protective Effects of Hyperbaric Oxygen Therapy on Brain Injury by Regulating the Phosphorylation of Drp1 Through ROS/PKC Pathway in Heatstroke Rats Ni XX,Cell Mol Neurobiol,2020
- Resveratrol improves left ventricular remodeling in chronic kidney disease via Sirt1-mediated regulation of FoxO1 activity and MnSOD expression Li P, et al,Biofactors,2019
- Isoorientin plays an important role in alleviating Cadmium-induced DNA damage and G0/G1 cell cycle arrest Shihao Chen, et al,Ecotoxicology and Environmental Safety,2019
- Galangin Activates Nrf2 Signaling and Attenuates Oxidative Damage, Inflammation, and Apoptosis in a Rat Model of Cyclophosphamide-Induced Hepatotoxicity Aladaileh SH, et al,Biomolecules,2019
- Amelioration of fenitrothion induced oxidative DNA damage and inactivation of caspase-3 in the brain and spleen tissues of male rats by N-acetylcysteine Rasha T. Alam,Life Sciences,2019
- The beneficial effect of α-tocopherol succinate supplementation on DNA oxidation induced by intensive exercise training Hossein TaheriChadorneshin, et al,Sport Sciences for Health,2018
- Aplysin Protects Against Alcohol┸\Induced Liver Injury Via Alleviating Oxidative Damage and Modulating Endogenous Apoptosis┸\Related Genes Expression in Rats Na Ge.et al,Journal of Food Science,2018
- PM2.5 induces male reproductive toxicity via mitochondrial dysfunction, DNA damage and RIPK1 mediated apoptotic signaling pathway Jin Zhang.et al,Science of The Total Environment,2018
- The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in a Streptozotocin-Induced Diabetic Model Gehan A Hegazy.et al,Biomedical and Pharmacology,2018
- Puerarin Mitigates Diabetic Hepatic Steatosis and Fibrosis by inhibiting TGF-B signaling pathway activation in Type 2 Diabetic Rats Biyu Hou.et al,/,/
- Inhibition of Rac1 ameliorates neuronal oxidative stress damage via reducing Bcl-2/Rac1 complex formation in mitochondria through PI3K/Akt/mTOR Pan Y.et al,Exp Neurol,2017
- Nandrolone administration with or without strenuous exercise promotes overexpression of nephrin and podocin genes and induces structural and functional Tofighi A.et al,Toxicol Lett,2017
- Restoration of Spermatogenesis Using a New Combined Herbal Formula of Epimedium koreanum Nakai and Angelica gigas Nakai in an Luteinizing Hyun Jun Park.et al,World J Mens Health,2017
- Fucoidan Protects Dopaminergic Neurons by Enhancing the Mitochondrial Function in a Rotenone-induced Rat Model of Parkinson’s Disease Zhang Li.et al,Aging Dis,2017
- Dietary Selenium Deficiency or Excess Reduces Sperm Quality and Testicular mRNA Abundance of Nuclear Glutathione Peroxidase 4 in Rats Zhou JC.et al,J Nutr.,2017
- Rescue effects of ginger extract on dose dependent radiation-induced histological and biochemical changes in the kidneys of male Wistar rats Saberi H.et al,Biomed Pharmacother.,2017
- Ginger extract mitigates ethanol-induced changes of alpha and beta ? myosin heavy chain isoforms gene expression and oxidative stress in the heart of male wistar rats Alireza Shirpoor.et al,DNA Repair,2017
- Effect of intensive endurance training on rat brain and hepatic 8-oxoguanine DNA glycosylase and 8-hydroxy-2'-deoxyguanosine levels Mohammad Afzalpour.et al,Journal of Applied Pharmaceutical Science ,2016
- Metabonomics revealed xanthine oxidase-induced oxidative stress and inflammation in the pathogenesis of diabetic nephropathy Liu J.et al,Anal Bioanal Chem.,2015
- Assessment of benzene induced oxidative impairment in rat isolated pancreatic islets and effect on insulin secretion Haji Bahadar et al,Environmental Toxicology and Pharmacology,2015
- The effect of prenatal exposure to 900-megahertz electromagnetic field on the, 21-old-day rat testicle Hanc谋 H et al,Reprod Toxicol,2013
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靶点详情
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最新研究进展:8-OHdG是DNA氧化损伤的标志物,最新研究发现,其与肺癌、肝癌、结直肠癌、卵巢癌等多种癌症的发生和发展有关。研究人员通过对癌症患者和对照组进行对比分析,发现8-OHdG的水平在癌症患者中明显升高,因此可以作为肿瘤的生物标志物之一。此外,8-OHdG的水平还可以反映环境暴露和生活方式对人体DNA的影响。最新研究表明,8-OHdG的水平与吸烟、饮酒、肥胖、缺乏锻炼等不良生活习惯有关。因此,8-OHdG的测量可以用作评估生活方式和环境暴露的生物标志物。另外,一些研究还表明,8-OHdG可能与一些神经退行性疾病的发生和发展有关,包括帕金森病和阿尔茨海默病。在这些疾病中,氧化应激和DNA氧化损伤可能是致病机制之一,而8-OHdG的测量可以用作评估疾病进展和治疗效果的生物标志物。
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