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  4. Human lysophosphatidic acid (LPA) Elisa kit

Human lysophosphatidic acid (LPA) Elisa kit

  • 中文名称：
    人溶血磷脂酸(LPA)酶联免疫试剂盒
  • 货号：
    CSB-EQ028005HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human LPA ELISA Kit was designed for the quantitative measurement of Human LPA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.9 ng/mL-250 ng/mL and the sensitivity is 3.9 ng/mL.

  • 缩写：
    LPA
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.9 ng/mL-250 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 92-102  
    1:200 Average % 94  
    Range % 91-98  
    1:400 Average % 102  
    Range % 98-106  
    1:800 Average % 87  
    Range % 82-95  
  • 回收率：
    The recovery of human LPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 80-96  
    EDTA plasma (n=4) 96 92-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    250 0.090 0.089 0.090    
    125 0.147 0.154 0.151    
    62.5 0.255 0.267 0.261    
    31.25 0.500 0.491 0.496    
    15.6 0.757 0.762 0.760    
    7.8 1.068 1.059 1.064    
    3.9 1.544 1.447 1.496    
    0 2.623 2.655 2.639    
  • 本试剂盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with human LPA.
    • Standard(10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-conjugated LPA antibody(100 x concentrate) (1 x 60 μl) --Bind to the LPAA, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated LPAantibody solution.
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
    • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

I want to know wihich type of plastic plates are included in this elisa kit to evaluate our optical density.

A:
Thanks for your inquiry!
The plate of our kit CSB-EQ028005HU is with 8 wells× 12 strips.
Pls just use the general microplate reader with the 450nm wavelength.
Any question, pls feel free to contact me.
Q:

CSB-EQ028005HU​ - Human lysophosphatidic acid (LPA) Elisa kit Knowing that LPA is also found in mice, do you think it will also work for mice cells?

A:
Thanks for your inquiry! This kit is specific to human. For different species there is matrix difference, so we don't suggest you use this kit to test mouse samples.