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Human heparan sulfate,HS ELISA Kit

  • 中文名称：
    人硫酸类肝素(HS)酶联免疫试剂盒
  • 货号：
    CSB-E09585h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human heparan sulfate (HS) ELISA Kit was designed for the quantitative measurement of Human heparan sulfate (HS) protein in serum, plasma, urine, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-8000 ng/mL and the sensitivity is 31.25 ng/mL.

  • 别名：
    N/A
  • 缩写：
    HS
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, tissue homogenates
  • 检测范围：
    31.25 ng/mL-8000 ng/mL
  • 灵敏度：
    31.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 本试剂盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with HS.
    • Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-human HS Antibody (100 x concentrate) (1 x 60 μl)
    • Antibody Diluent (1 x 10 ml) --Dilute the anti-human LRG1
    • HRP-conjugated goat-anti-mouse HS antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
    • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated HS antibody.
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
    • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

What does Cusabio recommend for sample dilution buffer? None is supplied in the kit and I want to know what should be diluting my samples with to be compatible with the cusabio ELISA kit. (CSB-E09585h)
Is it distilled water or some other buffer?

A:
Thanks for your inquiry. We recommend you use normal saline or PBS.