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  4. Recombinant Rat Sodium/bile acid cotransporter (Slc10a1)

Recombinant Rat Sodium/bile acid cotransporter (Slc10a1)

  • 中文名称：
    大鼠Slc10a1重组蛋白
  • 货号：
    CSB-CF021373RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Slc10a1
  • Uniprot No.：
  • 别名：
    Slc10a1; Ntcp; Sodium/bile acid cotransporter; Na(+/bile acid cotransporter; Na(+/taurocholate transport protein; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-362
  • 氨基酸序列
    MEVHNVSAPFNFSLPPGFGHRATDKALSIILVLMLLLIMLSLGCTMEFSKIKAHLWKPKG VIVALVAQFGIMPLAAFLLGKIFHLSNIEALAILICGCSPGGNLSNLFTLAMKGDMNLSI VMTTCSSFSALGMMPLLLYVYSKGIYDGDLKDKVPYKGIMISLVIVLIPCTIGIVLKSKR PHYVPYILKGGMIITFLLSVAVTALSVINVGNSIMFVMTPHLLATSSLMPFSGFLMGYIL SALFQLNPSCRRTISMETGFQNIQLCSTILNVTFPPEVIGPLFFFPLLYMIFQLAEGLLI IIIFRCYEKIKPPKDQTKITYKAAATEDATPAALEKGTHNGNIPPLQPGPSPNGLNSGQM AN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
  • 基因功能参考文献：
    1. Administration of glucocorticoids (GCs) enhanced liver bile acid uptake by increasing the expression of the major hepatocyte basolateral bile transporter (Ntcp)in rats. The reduced expression of a bile acid synthesis rate-controlling enzyme, Cyp7a1, suggests that treatment with GCs suppressed hepatic bile acid synthesis. PMID: 27133208
    2. Ntcp plasma membrane localization is regulated by hyperosmolarity and tauroursodeoxycholate PMID: 26306036
    3. Taurocholate (TCA) for Ntcp. PMID: 25305012
    4. This study identified a motif which is critical for Ntcp plasma membrane localization. PMID: 24008362
    5. hepatic transporters Ntcp and Mrp2 are downregulated in rodent models of necrotizing enterocolitis PMID: 23125159
    6. sustained repression and translocation of Ntcp and the expression of Mrp4 at the basolateral membrane seem to be responsible for the high blood bile acids levels after massive hepatectomy PMID: 21167233
    7. Ca(2+)-dependent PKCs induce vesicular retrieval of Ntcp, thereby reducing bile salt uptake. Short-term downregulation of Ntcp by endocytosis may be important mechanism to protect hepatocytes from effects of toxic intracellular bile salt concentrations. PMID: 20539008
    8. translocation requires activation of protein kinase B PMID: 12034724
    9. Pregnancy is associated with reduced Ntcp expression and function in the rat PMID: 12547402
    10. Results show that loop amino acids are required for sodium-dependent substrate translocation by the sodium-dependent taurocholate cotransporting polypeptide Ntcp. PMID: 12631271
    11. Substrate specificity of ntcp is considerably broader than previously suspected and caution extrapolation of transport data obtained in vitro to physiological function in vivo. PMID: 12842829
    12. TNF-alpha represents the master cytokine responsible for HNF1-dependent down-regulation of Ntcp in CCl(4)-induced toxic liver injury and IL-1 beta predominates in a complex signaling network of Ntcp regulation in cholestatic liver injury PMID: 12883478
    13. Physiological significance of cross-talk between ERalpha and Stat5a in liver, in which both proteins are expressed. Novel mechanism by which expression of ntcp, an important hepatic bile acid transporter, can be regulated by multiple hormones. PMID: 14684617
    14. Conserved NTCP/Ntcp 5'-regulatory region transcription regulation differs among species and is not directly regulated by small heterodimer partner. Bile acids may regulate NTCP/Ntcp indirectly by modulating nuclear factor regulation of gene expression. PMID: 14701722
    15. Data show that endogenous thyroxine but not corticosterone regulates the developmentally timed appearance of the apical sodium-dependent bile acid transporter. PMID: 14711893
    16. pituitary hormones play an essential role in the sexually dimorphic control of Ntcp expression in adult rat liver and in the mediation of estrogen effects. PMID: 15361361
    17. Results suggest that the Ser-226 in the third cytoplasmic loop of Ntcp is phosphorylated and cAMP may increase Ntcp translocation to the cell membrane by dephosphorylating Ntcp at this site. PMID: 16027164
    18. analysis of functionally relevant residues of the rat ileal apical sodium-dependent bile acid cotransporter PMID: 16608845
    19. In hypoxic liver tissue, a marked reduction in the mRNA levels of Na(+)-taurocholate-cotransporting polypeptide (Ntcp was detected before bile duct proliferation occurred PMID: 17615179
    20. Upregulation of the basolateral bile acid transporters Ntcp, Oatp1, and Oatp2 in fasted rats is mediated via the HNF4alpha/PGC-1alpha pathway. PMID: 17640976
    21. Report PKCepsilon-dependent and -independent effects of taurolithocholate on PI3K/PKB pathway and taurocholate uptake in HuH-NTCP cell line. PMID: 19815625

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Bile acid:sodium symporter (BASS) (TC 2.A.28) family
  • 组织特异性：
    Liver and kidney.
  • 数据库链接：

    KEGG: rno:24777

    STRING: 10116.ENSRNOP00000007825

    UniGene: Rn.9913