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  4. Recombinant Rat Inward rectifier potassium channel 2 (Kcnj2)

Recombinant Rat Inward rectifier potassium channel 2 (Kcnj2)

  • 中文名称：
    大鼠Kcnj2重组蛋白
  • 货号：
    CSB-CF737346RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Kcnj2
  • Uniprot No.：
  • 别名：
    Kcnj2; Irk1; Inward rectifier potassium channel 2; Inward rectifier K(+ channel Kir2.1; IRK-1; RBL-IRK1; Potassium channel, inwardly rectifying subfamily J member 2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-427
  • 氨基酸序列
    MGSVRTNRYSIVSSEEDGMKLATMAVANGFGNGKSKVHTRQQCRSRFVKKDGHCNVQFIN VGEKGQRYLADIFTTCVDIRWRWMLVIFCLAFVLSWLFFGCVFWLIALLHGDLDASKESK ACVSEVNSFTAAFLFSIETQTTIGYGFRCVTDECPIAVFMVVFQSIVGCIIDAFIIGAVM AKMAKPKKRNETLVFSHNAVIAMRDGKLCLMWRVGNLRKSHLVEAHVRAQLLKSRITSEG EYIPLDQIDINVGFDSGIDRIFLVSPITIVHEIDEDSPLYDLSKQDIDNADFEIVVILEG MVEATAMTTQCRSSYLANEILWGHRYEPVLFEEKHCYKVDYSRFHKTYEVPNTPLCSARD LAEKKYILSNANSFCYENEVALTSKEEEDSENGVPESTSTDSPPGIDLHNQASVPLEPRP LRRESEI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
  • 基因功能参考文献：
    1. Report coupling of cardiac Kir2.1 and Kir2.2 subunits with the plasma membrane-bound AKAPs 15 and 79. Cardiac membrane-associated AKAPs are a functionally essential part of the regulatory cascade determining IK1 current function. PMID: 28331977
    2. Moreover, the in vivo study showed that rat-VSMC switched to proliferative phenotypes and that knockdown of Kir2.1 significantly inhibited neointimal formation after rat carotid injury. Kir2.1 may be a potential therapeutic target in the treatment of cardiovascular diseases, such as atherosclerosis and restenosis following percutaneous coronary intervention. PMID: 27387235
    3. Th1 response regulates Kir2.1 expression in infarcted hearts and valsartan regulates Th1 response to improve cardiac ion channel remodeling following myocardial infarction. PMID: 26566151
    4. Kir2 channels composed of Kir2.1-2.3 subunits are expressed in neuronal and glial cells in the DRG and spinal cord, contributing to sensory transduction and motor control. PMID: 26854211
    5. Immunohistochemical analysis reveals the expression of Kir2.1, Kir2.2, and Kir2.3 within spontaneously bursting neurons that regulate the level of pacemaker activity within the developing superficial dorsal horn. PMID: 23426663
    6. The inward rectifier potassium current was clearly inhibited after the inhibition of KCNJ2 gene expression by RNA interference. PMID: 22426100
    7. Vascular smooth muscle Kir2.1 channels are involved in the nitroprusside-induced dilation of rat-tail small arteries. PMID: 14993195
    8. In PCR analyses using isolated juxtaglomerular cells, the mRNA for Kir2.1 (Kcnj2) and Kir2.2 (Kcnj12) was detected. Kir2.1 is the dominant component of the channel. PMID: 15284349
    9. The expression of Kir4.1 mRNA was reduced by 55% after ischemia while the expression of Kir2.1 mRNA was not altered. PMID: 16330144
    10. Immunochemical staining verified that both pericytes and endothelial cells of descending vasa recta express K(IR)2.1, K(IR)2.2, and K(IR)2.3 subunits. PMID: 17670900
    11. In addition, LV-mediated overexpression of Kir2.1 opens up the prospect of studying the functional role of inward rectifier K(+) current in cardiac arrhythmias. PMID: 17675572
    12. Biophysical properties of K(IR) (comprised K(IR)2.1 and 2.2 subunits) have a substantive role in enabling agonists to alter the electrical state of a multilayered artery. PMID: 18063660
    13. Experiments demonstrated surface expression of Kir2.1-containing channels in astrocytes and in populations of pyramidal and nonpyramidal cells. Findings for astrocytes were not consistent with predictions for spatial buffering over substantial distance. PMID: 18076085

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein. Membrane; Lipid-anchor.
  • 蛋白家族：
    Inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ2 subfamily
  • 组织特异性：
    Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.
  • 数据库链接：

    KEGG: rno:29712

    STRING: 10116.ENSRNOP00000006254

    UniGene: Rn.44415