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  4. Recombinant Rat ATP-sensitive inward rectifier potassium channel 11 (Kcnj11)

Recombinant Rat ATP-sensitive inward rectifier potassium channel 11 (Kcnj11)

  • 中文名称：
    大鼠Kcnj11重组蛋白
  • 货号：
    CSB-CF012049RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Kcnj11
  • Uniprot No.：
  • 别名：
    Kcnj11; ATP-sensitive inward rectifier potassium channel 11; BIR; Inward rectifier K(+ channel Kir6.2; Potassium channel, inwardly rectifying subfamily J member 11
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-390
  • 氨基酸序列
    MLSRKGIIPEEYVLTRLAEDPTEPRYRTRERRARFVSKKGNCNVAHKNIREQGRFLQDVF TTLVDLKWPHTLLIFTMSFLCSWLLFAMVWWLIAFAHGDLAPGEGTNVPCVTSIHSFSSA FLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGLMINAIMLGCIFMKTAQAHRRAET LIFSKHAVITLRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPM ENGVGGNSIFLVAPLIIYHVIDSNSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQA RTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTVKVPTPLCTARQLDEDRSLLDALT LASSRGPLRKRSVAVAKAKPKFSISPDSLS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.
  • 基因功能参考文献：
    1. The E23K variant of the KATP channel increased the susceptibility to ventricular arrhythmia under acute ischemia stress. PMID: 28082085
    2. Data show that sarcolemmal ATP-sensitive K+ (sarcKATP) channel mutant subunit Kir6.2AAA transfection inhibited the channel activity and significantly aggravated the apoptosis of myocytes induced by lipopolysaccharides (LPS). PMID: 27430376
    3. Findings suggest that Kir6.2 expression in the spinal cord is reduced in bone cancer pain and activation of Kir6.2 at the spinal level reduces pain hypersensitivity associated with bone cancer pain PMID: 24480471
    4. Kir6.2 plays a central role in ischemic preconditioning and calcium and mitochondrial homeostasis during stress, and the impact of decreasing channel number is deleterious to myocyte survival. PMID: 24014680
    5. The G156P mutation reduces the ability of PIP2 to stabilize the open state of Kir6.2 channels. PMID: 23700433
    6. Estradiol significantly upregulates the expression of KATP channel subunits and SUR1 activity in the brain cortices of ovariectomized rats. PMID: 23032400
    7. A set of the Linear Interaction Energy method parameters was defined that may enable prediction of the effects of additional Kir6.2 mutations within the ATP binding site on the affinity for ATP. PMID: 23219002
    8. Channels bearing Q52E mutation in Kir6.2 and E203C in SUR1 exhibit ATP sensitivity 100-fold higher than wild-type channels. PMID: 22802363
    9. Binding of HIF-2alpha to a putative hypoxia response element in Kir6.2 gene promoter is validated in adrenal glands from pups born to dams exposed to nicotine throughout gestation. PMID: 22403787
    10. Activation of astrocytic mitochondrial K(ATP) channels improves gap junction function in astrocytes via regulation of gap junction-coupled spatial buffering in the hippocampus. PMID: 21474909
    11. ATP activates ATP-sensitive potassium channels composed of mutant sulfonylurea receptor 1 and Kir6.2 with diminished PIP2 sensitivity. PMID: 21654216
    12. Identify a critical determinant in TMD0 that controls Kir6.2 gating by controlling channel sensitivity to PIP(2). Uncover a novel mechanism of K(ATP) channel inactivation involving aberrant functional coupling between SUR1 and Kir6.2. PMID: 21321069
    13. The activity of K(ATP) channels in hippocampal H19-7 neurons treated with or without short interfering RNAs (siRNAs) directed against Kir6.2 mRNA was investigated in this study. PMID: 21145327
    14. The gastric responses to hyperglycemia and tolbutamide were not observed in rats with Kir6.2 small interfering RNA-treated nodose ganglia. PMID: 21193530
    15. Kir6.2 was found in most rat brain neuronal populations. Both oligodendrocytes and astrocytes contain Kir6.2. It was found in the endoplasmic reticulum, Golgi apparatus and cell membranes of glia and neurons. PMID: 12007828
    16. activation of KATP channels in slow-twitch muscle is necessary for activation of the NKCC and cell volume restoration in hyperosmotic conditions. PMID: 14592811
    17. There is a direct interaction between Kir6.2 and a 65-amino-acid section of the cytoplasmic C-terminal region of rSUR2A between residues 1294 and 1358. PMID: 14672537
    18. KATP channels play an important role in glucose sensing and insulin secretion, and ribozyme Kir6.2-gene targeting is an effective approach for selective inhibition of functional expression of KATP channels. PMID: 15166124
    19. expression of Kir6.2 in the rat intestine is moderated by Isl-1 PMID: 15528203
    20. Zinc activated KATP in cells co-expressing rat Kir6.2 and SUR1 subunits. PMID: 15613469
    21. Kir6.2 to be widely expressed in all brain regions, suggesting that the Kir6.2 subunit forms the pore of the K-ATP channels in most neurons PMID: 15739238
    22. Mutations in this gene should be taken into consideration for not only permanent neonatal diabetes but also other forms of diabetes with milder phenotypes and later onset. PMID: 15784703
    23. These results suggest that 14,15-EET activates vascular K(ATP) channels through ADP-ribosylation of G(s)alpha. PMID: 16537788
    24. findings show that PNDM mutations, including Q52R, V59G, V59M, R201C, R201H, and I296L, not only reduce channel ATP sensitivity but also impair channel expression at the cell surface to varying degrees PMID: 16731837
    25. sulfonylureas also reverse trafficking defects caused by neonatal diabetes-associated Kir6.2 mutations in a way that is dependent on intact sulfonylurea binding sites in sulfonylurea receptor 1 further support this notion PMID: 16956886
    26. changes in hypothalamic Kir6.2 expression may be associated with the development of hyperinsulinaemia and hyperglycaemia PMID: 18001323
    27. HSP90 is involved in K ATP-mediated cytoprotection, possibly by promoting mitochondrial targeting of Kir6.2. PMID: 18006464
    28. the importance of Kir6.2 subunit-subunit interactions in K(ATP) channel gating and function and reveal a novel gating defect underlying CHI. PMID: 18250167
    29. observations are consistent with the hypothesis that testosterone modulates male sexual behavior by regulating K(+)(ATP) channels in the brain PMID: 18950632
    30. Kir6.2 was found to be positively regulated by ARNT at the mRNA and protein levels. PMID: 19141293

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ11 subfamily
  • 数据库链接：

    KEGG: rno:83535

    STRING: 10116.ENSRNOP00000028685

    UniGene: Rn.3985