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  4. Recombinant Mouse Threonylcarbamoyladenosine tRNA methylthiotransferase (Cdkal1)

Recombinant Mouse Threonylcarbamoyladenosine tRNA methylthiotransferase (Cdkal1)

  • 中文名称：
    Recombinant Mouse Threonylcarbamoyladenosine tRNA methylthiotransferase(Cdkal1)
  • 货号：
    CSB-CF852783MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Cdkal1
  • Uniprot No.：
  • 别名：
    Cdkal1; Threonylcarbamoyladenosine tRNA methylthiotransferase; CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t(6A37 methylthiotransferase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-578
  • 氨基酸序列
    MPSASCDVLLDDIEDIISQEDSKPQDRQFSRKHVFPKVRRRNTQKYLQEEPRPPSDSTIP GIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRN SIKKAQEENKKVVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQ KKDNGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVER AKQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGAMLRLGMTNPPYILEH LEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATD IICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKAEQVPAHVKKQRTKDLS RVFHSYNPYDHKIGERQQVLVTEESFDSKFYVAHNRFYEQVLVPKNPAFMGKMVEVDIYE SGKHFLKGQPVSETRVYTPSISKPLAKGEVSGLTKEFRNRLGNHPNGTSDTCPATQHGSA YSRMVLQMSQYDCALKVATGLALLALLLHFWPDSLLTM
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
  • 基因功能参考文献：
    1. Cdkal1 is necessary for normal mitochondrial morphology and function in adipose tissue. These results suggest that the type 2 diabetes susceptibility gene CDKAL1 has novel functions in regulating mitochondrial activity. PMID: 29031721
    2. CDKAL1 may affect such compensatory mechanisms regulating glucose homeostasis through interaction with diet PMID: 23173044
    3. Knockout mice with pancreatic beta-cell-specific lack of cdkal1 show pancreatic islet hypertrophy and impaired blood glucose control. PMID: 21908934
    4. Data show that Cdkal1 is a mammalian methylthiotransferase that biosynthesizes 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) in tRNA(Lys)(UUU) and that it is required for the accurate translation of AAA and AAG codons. PMID: 21841312
    5. CDKAL1 controls first-phase insulin exocytosis in beta cells by facilitating ATP generation, K(ATP) channel responsiveness and the subsequent activity of Ca(2+) channels through pathways other than CDK5-mediated regulation PMID: 21151568

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Methylthiotransferase family, CDKAL1 subfamily
  • 组织特异性：
    Expressed in pancreas, liver and skeletal muscle, especially in white muscle fibers.
  • 数据库链接：

    KEGG: mmu:68916

    STRING: 10090.ENSMUSP00000006353

    UniGene: Mm.212227