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  4. Recombinant Mouse Synaptotagmin-2 (Syt2)

Recombinant Mouse Synaptotagmin-2 (Syt2)

  • 中文名称：
    小鼠Syt2重组蛋白
  • 货号：
    CSB-CF023038MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Syt2
  • Uniprot No.：
  • 别名：
    Syt2; Synaptotagmin-2; Inositol polyphosphate-binding protein; IP4-binding protein; IP4BP; Synaptotagmin II; SytII
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-422
  • 氨基酸序列
    MRNIFKRNQEPNVAPATTTATMPLAPVAPADNSTESTGPGESQEDMFAKLKEKFFNEINKIPLPPWALIAMAVVAGLLLLTCCFCICKKCCCKKKKNKKEKGKGMKNAMNMKDMKGGQDDDDAETGLTEGEGEGEEEKEPENLGKLQFSLDYDFQANQLTVGVLQAAELPALDMGGTSDPYVKVFLLPDKKKKYETKVHRKTLNPAFNETFTFKVPYQELAGKTLVMAIYDFDRFSKHDIIGEVKVPMNTVDLGQPIEEWRDLQGGEKEEPEKLGDICTSLRYVPTAGKLTVCILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTVKKKTLNPYFNESFSFEIPFEQIQKVQVVVTVLDYDKLGKNEAIGKIFVGSNATGTELRHWSDMLANPRRPIAQWHSLKPEEEVDALLGKNK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties. May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Plays a role in dendrite formation by melanocytes.; (Microbial infection) Receptor for C.botulinum neurotoxin type B (BoNT/B, botB); interaction is improved in the presence of gangliosides. The toxin binds via the vesicular domain (residues 47-60).; (Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG); gangliosides are not required for (or only very slightly improve) binding to a membrane-anchored receptor fragment. The toxin binds via the vesicular domain (residues 47-55).
  • 基因功能参考文献：
    1. s propose that the strong reduction of Syt2 and SV2B are key factors of the functional synaptic alteration and that the physiological downregulation of Syt1 plays a determinant role in muscle vulnerability in SMA PMID: 28173138
    2. Deletion of Syt2 severely reduced and delayed disynaptic inhibition following parallel fiber stimulation and as release sensor at GABAergic basket cell-Purkinje cell synapses ensures fast and efficient feedforward inhibition in cerebellar microcircuit. PMID: 28099850
    3. aM inhibitors or the inhibition of the Ca(2+)-calmodulin-Munc13-1 signaling pathway only impaired the uptake of Syt2 while leaving membrane retrieval intact, indicating different recycling mechanisms for membranes and vesicle proteins. Our data identify a novel mechanism of stimulus- and Ca(2+)-dependent regulation of coordinated endocytosis of synaptic membranes and vesicle proteins. PMID: 27154627
    4. The 2.3-A structure of a ternary complex of botulinum neurotoxin type B bound to both its protein receptor synaptotagmin II and its ganglioside receptor GD1a, is reported. PMID: 23807078
    5. Syt2 make it an excellent marker for analyzing the development and plasticity of perisomatic inhibitory innervations onto both excitatory and inhibitory neurons in the visual cortex. PMID: 22539967
    6. Syt2 increases the dynamic range of synapses by driving release with a high Ca(2)+ cooperativity, as well as by suppressing a remaining, near-linear Ca(2)+ sensor. PMID: 21338883
    7. Transient expression of Syt2 is no longer detected in cochlear inner hair cells (IHCs) after the onset of hearing, indicating that the most common calcium-sensors in central nervous system synapses are not involved in mature IHC ribbon synapse. PMID: 20926654
    8. SYT2 was a negative regulator of chemotaxis PMID: 20473299
    9. Synaptotagmins I and II act as nerve cell receptors for botulinum neurotoxin G PMID: 15123599
    10. The readily releasable pool was rescued by Syt2 overexpression. The kinetics of fusion was slightly slower than in cells expressing Syt1. Syt2 has a lower Ca2+ affinity for phospholipid binding than Syt1 because of a difference in the C2A domain. PMID: 16407561
    11. Data suggest that synaptotagmins 1 and 2 perform equivalent functions in the Ca2+ triggering of action potential-induced neurotransmitter release and in the restriction of spontaneous release. PMID: 16642042
    12. Synaptotagmin-2 is an essential synaptotagmin isoform that functions in concert with other synaptotagmins in the calcium-ion triggering of neurotransmitter release. PMID: 17192432
    13. Data show that lack of Synaptotagmin-2 in mast cells was not associated with increased spontaneous exocytosis. PMID: 19473977

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass membrane protein. Cytoplasm.
  • 蛋白家族：
    Synaptotagmin family
  • 数据库链接：

    KEGG: mmu:20980

    STRING: 10090.ENSMUSP00000112438

    UniGene: Mm.5102