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  4. Recombinant Mouse Phosphatidylcholine:ceramide cholinephosphotransferase 2 (Sgms2)

Recombinant Mouse Phosphatidylcholine:ceramide cholinephosphotransferase 2 (Sgms2)

  • 中文名称：
    小鼠Sgms2重组蛋白
  • 货号：
    CSB-CF866682MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Sgms2
  • Uniprot No.：
  • 别名：
    Sgms2; Phosphatidylcholine:ceramide cholinephosphotransferase 2; Sphingomyelin synthase 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-365
  • 氨基酸序列
    MDIIETAKLEGHLESQTNDSTNTYTSPTEAVEEEGKNGKGKPKTLSNGLRKGAKKYPDYI QISMPNDSKNKFPLEWWKTGIAFVYALFNLILTTVMITVVHERVPPKELSPPLPDKFFDY FDRVKWAFSVSEINGMVLVGLWITQWLFLRYKSIVGRRFFFIMGTLYLYRCITMYVTTLP VPGMHFQCAPKLNGDSQAKIQRILRLISGGGLSITGSHILCGDFLFSGHTVVLTLTYLFI KEYSPRHFWWYHLVCWLLSAAGIICILVAHEHYTVDVIIAYYITTRLFWWYHSMANEKNL KVSSQTNFLSRAWWFPIFYFFEKNVQGSIPCCFSWPLSWPPGCFKSSCRKYSRVQKIGED NEKST
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sphingomyelin synthase that primarily contributes to sphingomyelin synthesis and homeostasis at the plasma membrane. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction appears to depend on the levels of CER and DAG in the plasma membrane. Does not use free phosphorylcholine or CDP-choline as donors. Can also transfer phosphoethanolamine head group of phosphatidylethanolamine (PE) on to ceramide (CER) to form ceramide phosphoethanolamine (CPE). Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting. To a lesser extent, plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1. Required for normal bone matrix mineralization.
  • 基因功能参考文献：
    1. This study showed that MWM learning was deteriorated and associated with depression-like tendency in SMS2 KO mice. PMID: 27840247
    2. These results demonstrate that SMS2 deficiency inhibits DSS-induced colitis and subsequent colitis-associated colon cancer via inhibition of colon epithelial cell-mediated inflammation; therefore, inhibition of SMS2 may be a potential therapeutic target for human colitis and colorectal cancer. PMID: 28522594
    3. It was suggested that very long chain SM but not long chain SM were decreased in SMS2-deficient mice liver and plasma. And the exogenously added very long chain SM (d18:1/24:0) could activate macrophages directly, suggesting a novel role of plasma very long chain SM in modulating macrophage activation and resulting inflammation. PMID: 27836537
    4. These findings suggested that the degree of lung injury was reduced during the acute inflammatory reaction when NFkappaB was inhibited, and that the expression of sphingomyelin synthase 2 may affect the induction of the NFkappaB pathway by lipopolysaccharide through CD14. PMID: 27510408
    5. Study provides a mouse model for fetal alcohol spectrum disorder. Due to the accumulation of ceramide in vivo, SMS2 knockout mice allows to investigate how ceramide regulates alcohol-induced neural apoptosis. PMID: 26562048
    6. study provided evidence that genetic inhibition of SMS2 elevated glucose clearance through activation of glucose uptake into insulin-targeted tissues such as skeletal muscle by a mechanism independent of hepatic SMS2 PMID: 27151272
    7. Thus, imaging mass spectrometry can provide visual assessment of the contribution of SMS2 on acyl-chain- and region-specific sphingomyelin metabolism in the kidneys PMID: 27010944
    8. characterization of enzymes catalyzing ceramide phosphoethanolamine biosynthesis in mice PMID: 25667419
    9. study reveals that all mouse SMS family members (SMSr, SMS1, and SMS2) have CPE synthase activity PMID: 25605874
    10. Increasing ceramide levels in the liver can be achieved by SMS2 inhibition to prevent liver steatosis via PPARgamma suppression. PMID: 23640498
    11. Data show that LCAT activity was significantly higher in long chain base biosynthesis protein 2 (Sptlc2)+/- and sphingomyelin synthase 2 (Sms2)-/- mice, but markedly lower in ApoE-/- and Ldlr-/- mice. PMID: 22370449
    12. SMS2 deficiency decreases atherosclerosis and inhibits inflammation in mice. PMID: 20717634
    13. Defects in the stria vascularis with reductions in endocochlear potentials together with hair cell dysfunction may account, at least partially, for hearing impairments in SMS1-/- mice. PMID: 22641779
    14. SMS2 over-expression was probably associated with increased expression of aortic inflammatory biomarkers, as well as decreased numbers of CD34/KDR-positive cells, CACs and CFUs in circulation PMID: 22538014
    15. Ablation of CerS2 causes changes in intrinsic membrane curvature, leading to strong morphological alterations that promote vesicle adhesion, membrane fusion, and tubule formation. PMID: 22231783
    16. SMS1 and SMS2 play distinct roles in regulating local sphingomyelin clustering. PMID: 22209789
    17. A deficiency of SMS2 can diminish the extent of pulmonary edema and lung injury. PMID: 21191108
    18. SMS2 (Sphingomyelin synthase 2) physiologically contributes to de novo Sphingomyelin biosynthesis and plasma membrane Sphingomyelin levels PMID: 18566297
    19. Sphingomyelin synthase 2 is one of the determinants for plasma and liver sphingomyelin levels in mice. PMID: 19286635
    20. Sphingomyelin synthase (SMS)2 deficiency in the macrophages reduces atherosclerosis in mice. PMID: 19590047

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Sphingomyelin synthase family
  • 组织特异性：
    Highest expression is detected in cortical bone, followed by vertebrae, kidney and liver. Expression levels are very low in spleen, muscle, heart, brown fat and thymus. Expressed in macrophages.
  • 数据库链接：

    KEGG: mmu:74442

    STRING: 10090.ENSMUSP00000087713

    UniGene: Mm.273360