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  4. Recombinant Mouse N-acetylaspartate synthetase (Nat8l)

Recombinant Mouse N-acetylaspartate synthetase (Nat8l)

  • 中文名称：
    小鼠Nat8l重组蛋白
  • 货号：
    CSB-CF663658MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Nat8l
  • Uniprot No.：
  • 别名：
    Nat8l; N-acetylaspartate synthetase; NAA synthetase; N-acetyltransferase 8-like protein; Protein Shati
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-299
  • 氨基酸序列
    MHCGPPDMVCETKIVATEDHEALPGAKKDALLVAAGAMWPPLPAAPGPAAAPPPAAGPQP HGGTGGAGPPEGRGVCIREFRAAEQEAARRIFYDGILERIPNTAFRGLRQHPRTQLLYAL LAALCFAVTRSLLLTCLVPAGLLALRYYYSRKVILAYLECALHTDMADIEQYYMKPPGSC FWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKSIAKALGRRVLEFAMLHNYSA VVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake.
  • 基因功能参考文献：
    1. The findings of this study indicated that the striatal expression of N-acetylaspartate synthetase Shati/Nat8l plays a role in major depressive disorder via the metabotropic glutamate receptor 3-mediated functional control of the serotonergic neuronal system. PMID: 29020418
    2. Results showed that the Shati/Nat8L mRNA was increased by methamphetamine-induced CREB pathway via dopamine D1 receptor signaling in mouse nucleus accumbens. These findings may contribute to development of a clinical tool for methamphetamine addiction. PMID: 28319198
    3. Results showed that both Shati+/- and Shati-/- mice demonstrated lower levels of NAA in the brain than Shati+/+ mice, suggesting that SHATI predominantly regulates NAA content in the brain PMID: 26361739
    4. Study shows that Shati/Nat8l in the neuron may induce axon outgrowth by ATP synthesis and not through mGluR3 signaling. PMID: 26164607
    5. Dysregulated expression of Nat8L is associated with deficits in oxidative phosphorylation in 5xFAD model of Alzheimer disease. PMID: 25766789
    6. results indicate that Shati/Nat8l in the Nucleus Accumbens, but not in the dorsal striatum, plays a suppressive role in the behavioral responses to METHamphetamine by controlling the dopaminergic system via activation of group II mGlutamate receptors PMID: 24559655
    7. The results of this study showed that Deletion of NAT8L increases dopamine D1 receptor on the cell surface in the nucleus accumbens, accelerating methamphetamine dependence in mice. PMID: 24246274
    8. Nat8l impacts on the brown adipogenic phenotype PMID: 24155240
    9. Deficiency of SHATI may affect behavior. PMID: 22940080
    10. shati expression affects emoutional behaivors in mice PMID: 22708272
    11. Asp-NAT enzyme activity in Nat8l-transfected cells is more than 300 times that of the mock-transfected controls and shows an extraordinarily high enzymatic specificity for aspartate, with less than 1% of enzymatic activity using glutamate as substrate. PMID: 20385109
    12. Nat8l is highly specific to aspartate. It is localized in mitochondria and microsomes, with a pattern comparable to the localization of NAA in brain. It is similar to NAG synthetase in catalytic mechanism without the involvement of cysteine. PMID: 20385109
    13. Results indicated that NAT8L, but not NAT14, catalysed the synthesis of NAA from L-aspartate and acetyl-CoA. PMID: 19807691
    14. Roles of shati in methamphetamine (METH)-regulated behavioral alternations are likely to be mediated by its inhibitory effects on METH-induced increase of dopamine(DA) overflow in nucleus accumbens and METH-induced decrease in DA uptake in midbrain. PMID: 17626222

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  • 亚细胞定位：
    Cytoplasm. Membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Rough endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Camello family
  • 组织特异性：
    Expressed in brain, kidney, liver and spleen. In brain, present in neurons but not in astrocytes (at protein level). Expressed in brain, thymus and spleen.
  • 数据库链接：

    KEGG: mmu:269642

    STRING: 10090.ENSMUSP00000059313

    UniGene: Mm.274610