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  4. Recombinant Mouse Integrin beta-3 (Itgb3)

Recombinant Mouse Integrin beta-3 (Itgb3)

  • 中文名称：
    小鼠Itgb3重组蛋白
  • 货号：
    CSB-CF011885MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Itgb3; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD antigen CD61
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-787
  • 氨基酸序列
    ESNICTTRGVNSCQQCLAVSPVCAWCSDETLSQGSPRCNLKENLLKDNCAPESIEFPVSEAQILEARPLSSKGSGSSAQITQVSPQRIALRLRPDDSKIFSLQVRQVEDYPVDIYYLMDLSFSMKDDLSSIQTLGTKLASQMRKLTSNLRIGFGAFVDKPVSPYMYISPPQAIKNPCYNMKNACLPMFGYKHVLTLTDQVSRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVLPNDGHCHIGTDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVSLYQNYSELIPGTTVGVLSDDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCVGLKIGDTVSFSIEAKVRGCPQEKEQSFTIKPVGFKDSLTVQVTFDCDCACQAFAQPSSPRCNNGNGTFECGVCRCDQGWLGSMCECSEEDYRPSQQEECSPKEGQPICSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCNCGDCVCDSDWTGYYCNCTTRTDTCMSTNGLLCSGRGNCECGSCVCVQPGSYGDTCEKCPTCPDACSFKKECVECKKFNRGTLHEENTCSRYCRDDIEQVKELTDTGKNAVNCTYKNEDDCVVRFQYYEDTSGRAVLYVVEEPECPKGPDILVVLLSVMGAILLIGLATLLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surfaces. Fibrinogen binding enhances SELP expression in activated platelets. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling. ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition. ITGAV:ITGB3 act as a receptor for CD40LG.
  • 基因功能参考文献：
    1. Study results utilizing knockout mice suggest smooth muscle cells (SMCs) contribution to atherogenesis is regulated by integrin beta-3-mediated pathways in both SMCs and bone marrow-derived cells. PMID: 29802249
    2. inflammation induces expression of alphaVbeta3 Integrin and other proteins, astrocyte reactivity, and Thy-1 responsiveness. Importantly, ectopic control of beta3 Integrin levels modulates these responses regardless of inflammation. PMID: 28962574
    3. The integrin Beta3 Pro33 mutation disrupts the localization of 5-HT transporters to specific compartments within the synapse. PMID: 29038237
    4. our finding that CD61 is conservative in defining HECs both in vitro for hPSC differentiation and in vivo for mouse embryo provides valuable information on how to define and access the bipotent HECs. PMID: 27746115
    5. The s showed the synergistic negative regulation of the pro-inflammatory cytokine interferon-gamma (IFNgamma) and beta3 integrin signaling in murine hematopoietic stem cells function by a novel definitive phenotyping of hematopoietic stem cells. PMID: 28673932
    6. This study provided evidence in platelets at both static and flow conditions that the calpain cleavage-related sequences of integrin beta3, i.e. T755NITYRGT762, R760GT762, and N756ITY759 participate in bidirectional, outside-in, and inside-out signaling, respectively and the association of c-Src or kindlin with beta3 integrin may regulate these processes. PMID: 27851790
    7. MiR-30a-5p suppresses tumor metastasis of human colorectal cancer by targeting ITGB3. PMID: 27576787
    8. suppression of beta3-integrin in mice leads to the activation of a neuropilin-1 (NRP1)-dependent cell migration pathway in endothelial cells via a mechanism that depends on NRP1's mobilisation away from mature focal adhesions following VEGF-stimulation PMID: 26159543
    9. Deletion of Itgb3 causes increased B-cell responses to TLR stimulation in vitro. PMID: 26965188
    10. integrin beta3-mediated signaling in smooth muscle cells links elastin deficiency and pathological stenosis, and inhibiting this pathway is an attractive therapeutic strategy for supravalvular aortic stenosis. PMID: 26858344
    11. The clathrin-mediated endocytic machinery combines with Dab2 to facilitate the endocytosis of RGD-integrin-beta3 clusters. PMID: 26507506
    12. studies identify alphavbeta3 as the primary integrin heterodimer mediating shear stress-induced proinflammatory responses and as a key contributor to early atherogenic inflammation PMID: 26212910
    13. Integrin beta 3 causes arteriovenous graft occlusion. PMID: 25614287
    14. Taken together, our results reveal that TD-26 is a novel antithrombotic compound exhibiting both integrin alphaIIbbeta3 inhibition and PI3K signaling blockage, with a low bleeding risk. PMID: 26278818
    15. Conformational activation of talin by PREL-1 triggers integrin-mediated cell adhesion. PMID: 25520155
    16. Conclude that tumour, rather than stromal, beta3 expression is essential and is required early for efficient spontaneous breast cancer metastasis to bone and soft tissues. PMID: 25430721
    17. in the presence of both Itgb3 and Slc6a4 heterozygozity, 5-HT uptake was similar to wild-type levels, revealing a significant Slc6a4 by Itgb3 genetic interaction PMID: 24083985
    18. Integrin beta3 mediates cerebrovascular remodelling during hypertension via Src/ClC-3 signalling pathway. PMID: 24611720
    19. Downregulation of TGF-beta1 and integrin beta3 impairs lymph node metastasis in a mouse model of non-small-cell lung cancer. PMID: 24884715
    20. We used MRI to image three genetic mouse models with single mutations implicated in autism: Neuroligin-3 R451C knock-in, Methyl-CpG binding protein-2 (MECP2) 308-truncation and integrin beta3 homozygous knockout. PMID: 24151012
    21. alphavbeta3-deficient mice displayed normal development of the virgin gland with no effect on luminal progenitors PMID: 25117682
    22. beta3-integrins have a role in RGD peptide-induced relaxation of pulmonary arteries and airways PMID: 24500921
    23. Angptl3 could induce actin filament rearrangement, mainly in lamellipodia formation, and that this process was mediated by integrin alpha(V)beta-mediated FAK and PI3K phosphorylation and Rac1 activation. PMID: 24294595
    24. beta3 integrin plays crucial role in blastocyst implantation; its co-expression with osteopontin is a biological marker for good endometrial receptivity PMID: 23870190
    25. In vivo analysis revealed that Spry4 regulated integrin beta3 levels in murine embryos and yolk sacs. PMID: 23955631
    26. C560Rbeta3 caused platelet integrin alphaII b beta3 to bind fibrinogen continuously, leading to hemorrhagic death consistent with Glanzmann thrombasthenia rather than a thrombotic state PMID: 23551977
    27. The results suggested that CD61 signaling is required for the proper growth of the cervical loop and for the promotion of the proliferation of pre-ameloblastic cells through Lgr5. PMID: 23553959
    28. Er'zhi Tiangui Granule could regulate the sequential expressions of integrinbeta3 and its ligand osteopontin to improve the mouse endometrial receptivity during controlled ovarian hyperstimulation. PMID: 23086489
    29. These results provide evidence for a direct role of integrin beta3 in the adhesion, migration and metastasis processes of mouse melanoma cells. PMID: 22895695
    30. Data indicate that beta3 integrin is required for Pyk2 cell surface localization and fibronectin accumulation. PMID: 22984613
    31. The pigment on the labial surface of mouse incisors is composed of Fe and that both anemia and reduction of iron-transporting proteins may cause the loss of pigmentation in CD61(-/-) mice. PMID: 23064962
    32. Genetic deletion of ITGbeta3 did not alter long-term potentiation or long-term depression in hippocampal slices. PMID: 22748100
    33. integrin beta(3)-deficiency in tumor stroma leads to an elevated IFP and lowered ECV that correlates with a more fibrotic ECM, underlining the role of the collagen network for carcinoma physiology PMID: 22479530
    34. These results indicate that both endothelial and platelet beta3 integrins contribute to extracellular PDI binding at the vascular injury site. PMID: 22653978
    35. Glycoprotein IIIa/IIb-targeted microbubbles specifically bind to activated platelets in vitro, allowing real-time molecular imaging of acute arterial thrombosis in a mouse model. PMID: 22647975
    36. beta3-integrin is required for differentiation in OC-2 cells, derived from E13 embryonic immortomouse inner ears. PMID: 22424110
    37. Integrin beta3 or its heterodimer alphavbeta3 is not critical for neutrophil migration into lungs infected with S. pneumoniae. PMID: 22350844
    38. we showed that the integrin-beta3 (CD61) signaling pathway was required for sustaining active TGFbeta signaling and self-renewal of TICs. PMID: 21996747
    39. the direct association between the cytoplasmic tails (CTs) of beta(3) and VEGFR2, is reported. PMID: 22363548
    40. study demonstrates that the epidermal growth factor receptor (EGFR) cooperates with beta3 integrin to regulate p190RhoGAP activity in mammary gland epithelial cells PMID: 21937717
    41. Integrin-beta3 plays a fundamental role in muscle regeneration through a regulation of macrophage infiltration and polarization leading to suppressed TGF-beta1 production. PMID: 22210777
    42. beta3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons. PMID: 22232691
    43. data suggest THY1 plays a role in cell adhesion via binding to integrin beta3 in ovaries; integrin beta3 colocalizes with THY1 in developing ovarian follicles PMID: 21228213
    44. The expression and regulation of beta3-integrin in skeletal muscles following chemical injury with cardiotoxin are reported. PMID: 21350117
    45. low dose aspirin alters the expression of endometrial LIF and integrin beta3 PMID: 21035850
    46. Integrin beta3 plays a vital role in preventing cardiomyocyte apoptosis under conditions of oxidative stress in vitro. PMID: 20393597
    47. controls proliferation and migration of vascular smooth muscle cells in response to vascular injury under hyperglycemic conditions PMID: 20184965
    48. beta3-integrins have cell-specific roles in complex biological processes PMID: 19933310
    49. These results show a function for the alphavbeta3 integrin in the regulation of protein permeability, selective for the heart capillaries. PMID: 19645751
    50. Data provide a proof for the concept that the function of TG2 is to stabilize accumulated integrin beta(3) concentration in the phagocytic cup. PMID: 19643138

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane. Cell junction, focal adhesion. Cell junction, synapse, postsynaptic cell membrane; Single-pass type I membrane protein. Cell junction, synapse.
  • 蛋白家族：
    Integrin beta chain family
  • 数据库链接：

    KEGG: mmu:16416

    STRING: 10090.ENSMUSP00000021028

    UniGene: Mm.87150