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Recombinant Mouse Insulin-induced gene 2 protein (Insig2)

  • 中文名称：
    小鼠Insig2重组蛋白
  • 货号：
    CSB-CF842029MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Insig2
  • Uniprot No.：
  • 别名：
    Insig2; Insulin-induced gene 2 protein; INSIG-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-225
  • 氨基酸序列
    MAEGETESPRPKKCGPYISSVTSQSVNVVIRGVVLFFIGVFLALVLNLLQIQRNVTLFPP DVITSIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINH ASAKVDFDNNFQFSLTLAALSVGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQY TSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVIAEKSHQE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR. Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. Binds oxysterol, including 22-hydroxycholesterol, 24-hydroxycholesterol, 25-hydroxycholesterol and 27-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum. In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi. Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG2 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2. Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase RNF139.
  • 基因功能参考文献：
    1. Intracellular biosynthesis of lipids and cholesterol by Scap and Insig in mesenchymal cells regulates long bone growth and chondrocyte homeostasis. PMID: 29899135
    2. INSIG2 is a negative regulator of SREBP, and acute glucocorticoid treatment decreased active SREBP during refeeding or in livers of Ob/Ob mice, both systems of elevated SREBP-1c-driven lipogenesis. PMID: 27568546
    3. These results indicate that HIF-mediated induction of Insig-2 and degradation of HMGCR are physiologically relevant events that guard against wasteful oxygen consumption and inappropriate cell growth during hypoxia. PMID: 28416613
    4. the essential role of Insig1,2 proteins in the sterol homeostasis of enterocytes. PMID: 24337570
    5. Insig deficiency in skin causes accumulation of cholesterol precursors, and this impairs normal hair development PMID: 20090767
    6. Data show that REV-ERBalpha participates in the circadian modulation of SREBP activity, and the expression of SREBP target genes involved in cholesterol and lipid metabolism via the cyclic transcription of Insig2. PMID: 19721697
    7. Liver-specific mRNA for Insig-2 down-regulated by insulin: implications for fatty acid synthesis. PMID: 12624180
    8. a vitamin D response element in the murine Insig-2 promoter may have a role in the differentiation of 3T3-L1 preadipocytes PMID: 15528275
    9. Results define Insig proteins 1 and 2 as essential elements of the feedback inhibition system of cholesterol synthesis in mouse liver. PMID: 16100574
    10. Insig2 identified as a strong candidate susceptibility gene for total plasma cholesterol levelsin inbred mice strains PMID: 16126366
    11. Treatment of pregnant mice with the HMG-CoA reductase inhibitor lovastatin reduced sterol synthesis in Insig2-knockout embryos and reduced the pre-cholesterol intermediates ameliorating the clefting. PMID: 16955138
    12. A critical gene involved in cholesterol homeostasis, Insig-2, was induced when mice or cultured cells were treated with FXRalpha agonists or infected with constitutively active FXRalpha. PMID: 17440045
    13. Peroxisome deficiency causes a complex phenotype because of hepatic SREBP-1c,-2/Insig-2a,b dysregulation associated with endoplasmic reticulum stress PMID: 19110480
    14. Silibinin inhibits adipocyte differentiation through a potential up-regulation of insig-1 and insig-2 at an early phase in adipocyte differentiation. PMID: 19360322

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    INSIG family
  • 组织特异性：
    Expressed in liver, testis, kidney, spleen, intestine, brain and adrenal gland.
  • 数据库链接：

    KEGG: mmu:72999

    STRING: 10090.ENSMUSP00000003818

    UniGene: Mm.27136