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  4. Recombinant Mouse Gap junction alpha-8 protein (Gja8)

Recombinant Mouse Gap junction alpha-8 protein (Gja8)

  • 中文名称：
    小鼠Gja8重组蛋白
  • 货号：
    CSB-CF009449MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Gja8
  • Uniprot No.：
  • 别名：
    Gja8; Gap junction alpha-8 protein; Connexin-50; Cx50; Lens fiber protein MP70
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-440
  • 氨基酸序列
    GDWSFLGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFVWGDEQSDFVCNTQQPGC ENVCYDEAFPISHIRLWVLQIIFVSTPSLMYVGHAVHHVRMEEKRKDREAEELCQQSRSN GGERVPIAPDQASIRKSSSSSKGTKKFRLEGTLLRTYVCHIIFKTLFEVGFIVGHYFLYG FRILPLYRCSRWPCPNVVDCFVSRPTEKTIFILFMLSVAFVSLFLNIMEMSHLGMKGIRS AFKRPVEQPLGEIAEKSLHSIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVETSPLSA KPFSQFEEKIGTGPLADMSRSYQETLPSYAQVGVQEVEREEPPIEEAVEPEVGEKKQEAE KVAPEGQETVAVPDRERVETPGVGKEDEKEELQAEKVTKQGLSAEKAPSLCPELTTDDNR PLSRLSKASSRARSDDLTI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Structural component of eye lens gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.
  • 基因功能参考文献：
    1. These results suggest that expression of Cx50D47A induces ER stress, triggering activation of the PERK-ATF4 pathway, which potentially contributes to the lens pathology and leads to increased expression of anti-apoptotic factors, allowing cell survival. PMID: 27317663
    2. data show that expression and phosphorylation of Cx46 and Cx50 are complementary in seminiferous tubules PMID: 26017495
    3. demonstrate, at the whole gap junction channel level, a crucial role of the surface charge properties in the first transmembrane/first extracellular border domain in determining the efficiency of ion permeation and the Vj gating of Cx50 PMID: 25260631
    4. The Gja8(R205G) mutation differentially impairs the functions of Cx50 and Cx46 to cause cataracts, small lenses and microphthalmia. PMID: 23300808
    5. D3 residue plays an essential role in unitary conductance of Cx50 gap junction channels PMID: 22404924
    6. Normal Cx50 function requires an intact PDZ domain-binding motif. PMID: 21965293
    7. Dense cataract and microphthalmia (dcm) in BALB/c mice is caused by mutations in the GJA8 gene. PMID: 20861565
    8. The N terminus of Cx50 is critical for both the V(j)-gating and the gamma(j), and the introduction of a positively charged Arg at the ninth position reduced the G(min) with a correlated disappearance of the substate at the single channel level. PMID: 20923641
    9. alpha8 connexin is required for proper fiber cell maturation and control of lens size PMID: 11782410
    10. intrinsic properties of Cx50 are required for cellular growth of lens cells PMID: 11786642
    11. Cx50-mediated communication is necessary to achieve peak mitosis. Possible novel mitogenic role for gap junctional coupling, connexin specific and independent of MAPK signaling. PMID: 15326140
    12. The hypothesis that the way in which V(j) gating reacts to CO(2) might be related to connexin 50 gating polarity is confirmed here. PMID: 15677379
    13. Results indicates connexin 50 expressed alone are recruited into different lipid environments and interaction of connexins and MP26 contributes to the overall organization of the fiber cells. PMID: 16740340
    14. The cataract-inducing S50P mutation in Cx50 dominantly alters the channel gating of wild-type lens connexins. PMID: 18003700
    15. MAPK signaling specifically modulates coupling mediated by Cx50 and that gap junctional communication and signal transduction pathways may interact in osmotic regulation during postnatal fiber development. PMID: 19321662
    16. A paper describing the knocking out of GJA8 (Cx50) in mice showed reduced lens growth and cataracts. Content is similar to the paper already cited (PMID 11782410) but published 4 years earlier. PMID: 9813099

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
  • 蛋白家族：
    Connexin family, Alpha-type (group II) subfamily
  • 组织特异性：
    Detected in eye lens (at protein level). Eye lens.
  • 数据库链接：

    KEGG: mmu:14616

    STRING: 10090.ENSMUSP00000049532

    UniGene: Mm.258183