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  4. Recombinant Mouse G protein-activated inward rectifier potassium channel 2 (Kcnj6)

Recombinant Mouse G protein-activated inward rectifier potassium channel 2 (Kcnj6)

  • 货号：
    CSB-CF012059MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Kcnj6; Girk2; Kcnj7; W; G protein-activated inward rectifier potassium channel 2; GIRK-2; Inward rectifier K(+ channel Kir3.2; Potassium channel, inwardly rectifying subfamily J member 6
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-425
  • 氨基酸序列
    MTMAKLTESMTNVLEGDSMDQDVESPVAIHQPKLPKQARDDLPRHISRDRTKRKIQRYVR KDGKCNVHHGNVRETYRYLTDIFTTLVDLKWRFNLLIFVMVYTVTWLFFGMIWWLIAYIR GDMDHIEDPSWTPCVTNLNGFVSAFLFSIETETTIGYGYRVITDKCPEGIILLLIQSVLG SIVNAFMVGCMFVKISQPKKRAETLVFSTHAVISMRDGKLCLMFRVGDLRNSHIVEASIR AKLIKSKQTSEGEFIPLNQSDINVGYYTGDDRLFLVSPLIISHEINQQSPFWEISKAQLP KEELEIVVILEGIVEATGMTCQARSSYITSEILWGYRFTPVLTMEDGFYEVDYNSFHETY ETSTPSLSAKELAELANRAEVPLSWSVSSKLNQHAELETEEEEKNPEELTERNGDVANLE NESKV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This potassium channel is controlled by G proteins. It plays a role in granule cell differentiation, possibly via membrane hyperpolarization. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
  • 基因功能参考文献：
    1. GIRK2 isoform balance within a neuron can impact the processing of afferent inhibitory input and associated behavior. PMID: 28487514
    2. Locomotor activity of the mice was not changed compared to that of GIRK2(floxed) mice, when tested in the open field PMID: 29180115
    3. Results indicate that the properties and inhibitory activity of dorsal raphe neurons are highly regulated by G protein-coupled inwardly rectifying K+ (GIRK) 2 subunit-containing channels, introducing GIRK channels as potential candidates for studying the pathophysiology and treatment of affective disorders. PMID: 28196855
    4. Increased Kcnj6 gene dose is necessary for synaptic and cognitive dysfunction in the animal model of Down Syndrome. PMID: 28342823
    5. The GABABR-coupled GIRK2 channel is necessary for the GABABR agonist-induced infantile spasms phenotype in the Ts mouse and may represent a novel therapeutic target for the treatment of infantile spasms in Down syndrome. PMID: 27462820
    6. It was concluded that GIRK2, through its dual responsiveness to G protein beta-gamma and Na+, mediates a form of neuronal inhibition that is amplifiable in the setting of excess electrical activity. PMID: 27074662
    7. cholesterol did not affect the unitary conductance of GIRK2, it significantly enhanced the frequency of channel openings. PMID: 28213520
    8. Results indicate that GIRK channels formed by GIRK2 subunits determine depression-related behaviors as well as basal and 5-HT1A receptor-mediated dorsal raphe neuronal activity PMID: 25956878
    9. This study showed that spontaneous GIRK2 mutations causing cerebellar pathology are impaired in motor functions during the neonatal period. PMID: 25907855
    10. This study demonstrated that the residue Ser-196 in Kir3.2 is involved in conferring PKC-mediated inhibition to the channel. PMID: 26490875
    11. In the ventral tegmental area, GABA neurons express both GIRK2 (and GIRK1) subunits. PMID: 25948263
    12. Gbeta-gamma and PIP2 must be present simultaneously to activate GIRK2. PMID: 25049222
    13. Ligand-operated activation of Kir3.2 appears to cause dilation of the pore at the cytoplasmic domain, and is regulated conformationally. PMID: 24244570
    14. A subcellular GIRK2c/GIRK3 pathway is identified that regulates excitability of ventral tegmental area dopamine neurons. PMID: 24811384
    15. The results of this study show that Girk2 gene mutation alters electric activity of LC neurons in vivo. PMID: 23040084
    16. Altogether, these findings shed new light on the developmental regulation of GIRK channels in the cerebellum PMID: 23261870
    17. In the dorsal horn of the developing rat, K(ir)3.1 and K(ir)3.2 were expressed at mature levels from birth. PMID: 23219908
    18. GIRK2 mediates the mGluR-sensitive current in unipolar brush cells PMID: 22528965
    19. GIRK2 is expressed in nearly every human pigmented neuron or mouse immunoreactive tyrosine hydroxylase-immunoreactive neuron in both the substantia nigra and ventral tegmental areas. PMID: 22252428
    20. biophysical analysis of conductance properties of the inwardly rectifying channel, Kir3.2 PMID: 23022491
    21. GIRK modulation occurs by channel assembly with R7-RGS/Gbeta5 complexes under allosteric control of R7 RGS-binding protein (R7BP). PMID: 23169654
    22. This study demonistrated that kcnj6 gene expression in mouse dorsal raphe nucleus PMID: 22534482
    23. Data describe the developmental regulation and subcellular diversity of neuronal GIRK/Kir3 channels, and support the contention that distinct subpopulations of GIRK channels exert separable influences on neuronal excitability. PMID: 22098295
    24. studies in two different mammalian species point to a conserved mechanism by which the GIRK2 inward-rectifying K(+) ion channels support sperm function during fertilization PMID: 22054410
    25. The mechanisms of regulation of GIRK by Galpha(i/o) using wild-type Galpha(i3) (Galpha(i3)WT) and Galpha(i3), were investigated. PMID: 21795707
    26. Altered neurotransmission in the limbic system of Girk2 knock-out mice involves secondary adaptations facilitating glutamatergic signaling. PMID: 20557431
    27. GIRK2 knockout mice demonstrate a transient hyperactive phenotype with initially high motor activity and slower habituation, increased levels of spontaneous locomotion during dark phase in their home cages, and impaired habituation in the open-field test. PMID: 11823889
    28. activation involved in mechanisms of analgesia PMID: 12493843
    29. contributes to channel-mediated postsynaptic signaling to opiate and alpha 2-adrenergic analgesia and analgesic sex differences PMID: 12496346
    30. results do not support the notion that Kir3.2 potassium channel mediates the capacity of inhaled anesthetics to produce immobility PMID: 12707131
    31. wvGIRK2 channels in native dopamine neurons are not permeable to Ca2+ and when activated by D@ and GABAB receptors mediate depolarization through VGCC channels PMID: 15240766
    32. Kir3.2-containing K+ channels on dendritic spines preferentially mediate the effect of GABA. PMID: 16624949
    33. Ts65Dn mice wirh Girk2 mutations display significantly increased hypothermic responses to 8-OH-DPAT, a serotonin receptor agonist. PMID: 16708025
    34. GIRK2 overexpression may adversely affect cerebellar circuitry in down syndrome animal model's vestibulocerebellum and dorsal cochlear nucleus. PMID: 16783527
    35. These results indicate that increased expression of GIRK2 containing channels have functional consequences that likely affect the balance between excitatory and inhibitory neuronal transmission. PMID: 17093127
    36. GIRK2 exhibited the most widespread and robust labeling in the cerebellum, with labeling particularly prominent in granule cells and Purkinje neurons , glogi cells,unipolar brush cells. PMID: 18088366
    37. Inhibition of adrenergic tone is required for the induction of dependence, and channels containing GIRK2 and GIRK3 serve as inhibitory gate. PMID: 18400906
    38. Results describe opioid-induced postsynaptic inhibition in locus coeruleus neurons from wild-type and GIRK2/GIRK3(-/-) mice at baseline and following chronic morphine treatment. PMID: 18702733
    39. Comparison of high-resolution structures of inwardly rectifying K(+) channels suggests a model for activation of GIRK channels using this hydrophobic alcohol-binding pocket. PMID: 19561601

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  • 相关疾病：
    Defects in Kcnj6 are the cause of the weaver (wv) phenotype. Homozygous animals suffer from severe ataxia that is obvious by about the second postnatal week. The cerebellum of these animals is drastically reduced in size due to depletion of the major cell type of cerebellum, the granule cell neuron. Heterozygous animals are not ataxic but have an intermediate number of surviving granule cells. Male homozygotes are sterile, because of complete failure of sperm production. Both hetero- and homozygous animals undergo sporadic tonic-clonic seizures.
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ6 subfamily
  • 组织特异性：
    Cerebellum, testes, cortex and substentia nigra.
  • 数据库链接：

    KEGG: mmu:16522

    STRING: 10090.ENSMUSP00000097108

    UniGene: Mm.328720