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  4. Recombinant Mouse E3 ubiquitin-protein ligase RNF128 (Rnf128)

Recombinant Mouse E3 ubiquitin-protein ligase RNF128 (Rnf128)

  • 中文名称：
    小鼠Rnf128重组蛋白
  • 货号：
    CSB-CF866675MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Rnf128
  • Uniprot No.：
  • 别名：
    Rnf128; Grail; Greul1; MNCb-3816; E3 ubiquitin-protein ligase RNF128; Gene related to anergy in lymphocytes protein; Goliath-related E3 ubiquitin-protein ligase 1; RING finger protein 128; RING-type E3 ubiquitin transferase RNF128
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    39-428
  • 氨基酸序列
    AEAVWTAYLNVSWRVPHTGVNRTVWELSEEGVYGQDSPLEPVSGVLVPPDGPGALNACNP HTNFTVPTVWGSTVQVSWLALIQRGGGCTFADKIHLASERGASGAVIFNFPGTRNEVIPM SHPGAGDIVAIMIGNLKGTKILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFFVSVSFFII TAATVGYFIFYSARRLRNARAQSRKQRQLKADAKKAIGKLQLRTLKQGDKEIGPDGDSCA VCIELYKPNDLVRILTCNHIFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSL QVPVSNEASNTASPHEEDSRSETASSGYASVQGADEPPLEEHAQSANENLQLVNHEANSV AVDVVPHVDNPTFEEDETPDQEAAVREIKS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
  • 基因功能参考文献：
    1. loss of Grail enhances anti-tumour reactivity and functionality of CD8(+) T cells. PMID: 28798332
    2. GRAIL expression is regulated by the IL2/mTOR/Otubain-1 axis in CD4 T cells during Trypanosoma cruzi infection. PMID: 28114324
    3. this study identified RNF128 as an E3 ligase for K63-linked ubiquitination and activation of TBK1 to RNA and DNA viruses PMID: 27776110
    4. Grail plays a critical role in controlling Th2 cell development through a negative feedback loop by targeting STAT6 for degradation. PMID: 25145352
    5. GRAIL-expressing T cells expressed regulatory T cell markers and showed suppressive effects in murine dextran sodium salt-induced colitis. PMID: 24356810
    6. findings reveal a novel association of the increased GRAIL expression with impaired CD4 T cell proliferation PMID: 24477910
    7. Data reveal that GRAIL regulates proteins involved in the actin cytoskeletal organization, thereby maintaining the unresponsive state of anergic T cells. PMID: 22016387
    8. GRAIL, by mediating TCR-CD3 degradation, regulates naive T cell tolerance induction and Treg cell function PMID: 20493730
    9. a biochemical pathway composed of GRAIL and/or GRAIL-interacting proteins is important in the development of the CD4 T cell anergic phenotype in vivo. PMID: 15210761
    10. GRAIL in the liver is essential for maintenance of normal glucose and lipid metabolism in living animals. PMID: 19060180
    11. the decline in Th2 cell responsiveness during chronic schistosomiasis is the net result of the upregulation of GRAIL expression in response to repeated antigen stimulation PMID: 19258704
    12. endogenous GRAIL regulates general cell cycle and proliferation of primary naive CD4 T cells. PMID: 19414743
    13. E3 ubiquitin ligase GRAIL controls primary T cell activation and oral tolerance PMID: 19805371
    14. Bone marrow reconstitution experiments with progenitor cells expressing biologically varied levels of GRAIL demonstrated diminished erythropoiesis and megakaryopoiesis if GRAIL (endogenous or forced) is maintained throughout hematopoiesis. PMID: 19887782

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  • 亚细胞定位：
    Endomembrane system; Single-pass membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region.
  • 组织特异性：
    Expressed in brain, kidney, heart, liver, ovary, testis and thymus. Expression increased as early as 4 hours by 5- to 7-fold in anergized cultures as compared to resting or activated cells.
  • 数据库链接：

    KEGG: mmu:66889

    STRING: 10090.ENSMUSP00000108649

    UniGene: Mm.27764