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  4. Recombinant Mouse Bestrophin-1 (Best1)

Recombinant Mouse Bestrophin-1 (Best1)

  • 中文名称：
    Recombinant Mouse Bestrophin-1(Best1)
  • 货号：
    CSB-CF002666MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Best1
  • Uniprot No.：
  • 别名：
    Best1; Bmd1; Vmd2; Bestrophin-1; Vitelliform macular dystrophy protein 2 homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-551
  • 氨基酸序列
    MTITYTNKVANARLGSFSSLLLCWRGSIYKLLYGEFLVFIFLYYSIRGLYRMVLSSDQQL LFEKLALYCDSYIQLIPISFVLGFYVTLVVSRWWSQYENLPWPDRLMIQVSSFVEGKDEE GRLLRRTLIRYAILGQVLILRSISTSVYKRFPTLHHLVLAGFMTHGEHKQLQKLGLPHNT FWVPWVWFANLSMKAYLGGRIRDTVLLQSLMNEVCTLRTQCGQLYAYDWISIPLVYTQVV TVAVYSFFLACLIGRQFLNPNKDYPGHEMDLVVPVFTILQFLFYMGWLKVAEQLINPFGE DDDDFETNWIIDRNLQVSLLSVDGMHQNLPPMERDMYWNEAAPQPPYTAASARSRRHSFM GSTFNISLKKEDLELWSKEEADTDKKESGYSSTIGCFLGLQPKNYHLPLKDLKTKLLCSK NPLLEGQCKDANQKNQKDVWKFKGLDFLKCVPRFKRRGSHCGPQAPSSHPTEQSAPSSSD TGDGPSTDYQEICHMKKKTVEFNLNIPESPTEHLQQRRLDQMSTNIQALMKEHAESYPYR DEAGTKPVLYE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Forms calcium-sensitive chloride channels. Permeable to bicarbonate.
  • 基因功能参考文献：
    1. Data suggest that the ion channels CaV1.3, bestrophin-1 and maxiK were identified as players in the regulation of photoreceptor outer segments (POS) phagocytosis by the retinal pigment epithelium (RPE). PMID: 26427483
    2. Best1-mediated astrocytic glutamate activates the synaptic N-methyl-D-aspartate receptor (NMDAR) and modulates NMDAR-dependent synaptic plasticity. PMID: 25645137
    3. Bestrophin-1 functions as an intracellular Cl channel which helps to accumulate and to release Ca(2+) from stores by conducting the counterion for Ca(2+). PMID: 24664688
    4. Best1, located at the microdomains near the synaptic junctions, has a significantly high permeability to glutamate in vivo. PMID: 24321245
    5. Results show that different mutations in Best1 cause differential effects on its localization and that this effect varies with the presence or absence of wild-type (WT) Best1. PMID: 23825107
    6. Astrocytic glutamate via Best1 channel targets and activates synaptic NMDA receptors. PMID: 23324492
    7. Upon activation of protease activated receptor 1 (PAR1), an increase in intracellular Ca2+ concentration leads to an opening of Best1 channels and subsequent release of glutamate in cultured astrocytes. PMID: 23062602
    8. Ultrastructural analyses demonstrate that TREK-1 is preferentially localized at cell body and processes, whereas Best1 is mostly found in microdomains of astrocytes near synapses. PMID: 23021213
    9. study reports that tonic inhibition in the cerebellum is due to GABA being released from glial cells by permeation through the Bestrophin 1 anion channel PMID: 20929730
    10. Retinal pigment bestrophin-1 possibly conducts Cl(-) as counter ion for Ca(2+) uptake into cytosolic Ca(2+) stores. PMID: 20411394
    11. Data demonstrate that bestrophin 1 is localized in the endoplasmic reticulum (ER), where it interacts with the ER-Ca(2+) sensor and can enhance Ca(2+) signaling and activation of Ca(2+)-dependent Cl(-) (TMEM16A) and K(+) (SK4) channels. PMID: 19823864
    12. cloning and characterization by combining biocomputational analyses and molecular genetic approaches PMID: 15218265
    13. We suggest the loss of vision associated with Best vitelliform macular dystrophy is not caused by the same pathologic process as the diminished light peak, but is caused by an unidentified effect of best-1 [VMD2] on other cellular processes. PMID: 16636205
    14. BEST1 may form the Ca2+-activated Cl(-) current, or it may be a component of a Cl(-) channel complex in epithelial tissues. PMID: 17003041
    15. suggest a role of bestrophin 1 and 2 for Ca(2+) dependent Cl(-) secretion in mouse airways PMID: 18652850
    16. This study provides the first characterization of the biophysical properties of mBest1 and a framework for the elucidation of the physiological role of bestrophins. PMID: 19426717
    17. Repression of cell proliferation, induction of Ca(2+)-dependent Cl(-) conductance, and expression of Best1 occurs during mesenchymal-to-epithelial transition once renal collecting duct cells polarize and terminally differentiate. PMID: 19470678
    18. Our data suggest that Best1 belongs to a group of genes upregulated by nerve injury and supports functional Ca(2+)-activated chloride current expression in injured sensory neurons PMID: 19675239
    19. The data of this study provided molecular evidence that the Bestrophin-1 channel is responsible for Ca2+-activated anion channel function in astrocytes. PMID: 19828819

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Basolateral cell membrane.
  • 蛋白家族：
    Bestrophin family
  • 数据库链接：

    KEGG: mmu:24115

    STRING: 10090.ENSMUSP00000113053

    UniGene: Mm.31577