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  4. Recombinant Human Type I iodothyronine deiodinase (DIO1)

Recombinant Human Type I iodothyronine deiodinase (DIO1)

  • 货号：
    CSB-CF343445HU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    DIO1
  • Uniprot No.：
  • 别名：
    DIO1; ITDI1; TXDI1; Type I iodothyronine deiodinase; 5DI; DIOI; Type 1 DI; Type-I 5'-deiodinase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-249
  • 氨基酸序列
    MGLPQPGLWLKRLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDN WIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSGQRCNIWEFMQGNRPLVLN FGSCTUPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHASDGWAFKNNMDIRNHQNLQDR LQAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYIIQEGRILYKGKSGPWNYNPEEV RAVLEKLHS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). Plays a role in providing a source of plasma T3 by deiodination of T4 in peripheral tissues such as liver and kidney.
  • 基因功能参考文献：
    1. this current study found that the serum DIO-1 concentration was inversely correlated with the concentration of TNF-alpha, which suggests that DIO-1 might inhibit the production of serum TNF-alpha in patients with chronic renal failure PMID: 30152254
    2. Multivariate linear regression analysis revealed that the DIO1 concentration and FT3 level were not associated with the concentration of serum 8-Isoprostane PMID: 29894998
    3. The expression of DIO1 on mRNA/protein levels in the depressive disorder patients was reduced in comparison to the control subjects. PMID: 29182613
    4. Minor variations in iodothyronine deiodinase 1 were associated with decreased well-being in the Korean hypothyroid population, whereas iodothyronine deiodinase 2 and 3 were not. PMID: 28466400
    5. wW observed that the DIO1 rs2235544 SNP modified the association between exposure to some of the organochlorine compounds (OCs) (specifically HCB and PCB153) and maternal thyroid hormone levels. These results strengthen the hypothesis that the deiodinase enzymes play a role in explaining the disruption of thyroid hormones in relation to exposure to OCs. PMID: 28395858
    6. data supports the notion that suppressed DIO1 expression and changes in local availability of thyroid hormones might favor a shift from a differentiated to a more proliferation-prone state of cancer tissues and cell lines PMID: 29272308
    7. results indicated that LXRalpha plays a specific and important role in activation of TH by regulating D1, and that LXRalpha binds to and regulates the hDIO1 promoter, competing with TRbeta on specific sequences within the promoter. PMID: 28617824
    8. The DIO1 gene is related to the depression. PMID: 27351946
    9. Functional variants within the DIO1 gene that affect triiodothyronine (T3) levels seem not to be associated with cognitive functions. PMID: 26866568
    10. All these results indicate that the oxidative stress downregulates the conversion of T4 to T3 through DIO1 function in HepG2 cells. PMID: 26947333
    11. Thyroid hormone deiodinases D1, D2, and D3 are differentially expressed in endothelial cells following thyroid hormone exposure. PMID: 25304215
    12. The pattern of expression and role of triiodothyronine (T3) receptors and type I 5'-deiodinase in breast carcinomas, benign breast diseases, lactational change, and normal breast epithelium. PMID: 24162265
    13. [review] Deiodinase type 1 polymorphisms particularly show moderate-to-strong relationships with thyroid hormone parameters, insulin-like growth factor (IGF)1 protein production, and risk for depression. PMID: 24878678
    14. a new mechanism of posttranscriptional regulation of DIO1 and show deregulation of DIO1 expression in pituitary adenoma, possibly resulting from disturbed expression of SF2/ASF. PMID: 23462647
    15. Data indicate that type 1 deiodinase is subject to catalysis-induced loss of activity. PMID: 22544951
    16. investigation of DIO1 and DIO2 activities in 66 thyroid tissue samples from follicular thyroid adenoma, toxic diffuse goiter, nontoxic multinodular goiter, papillary thyroid carcinoma, and surrounding normal tissues PMID: 22207295
    17. D1-C785T polymorphism correlates with the severity of pre-eclampsia PMID: 22339181
    18. Short interfering RNA-mediated knockdown of FOXA1 decreased the expression of DIO1 mRNA, but knockdown of both FOXA1 and FOXA2 restored it. PMID: 22067325
    19. a novel miRNA-mediated regulatory mechanism of Type 1 iodothyronine deiodinase expression in clear cell Renal Cell Carcinoma PMID: 21912701
    20. we report that SNP at the DIO1 variant rs11206244 is associated with lifetime major depression in White females in high-risk cohorts. PMID: 21563302
    21. [review] aims at presenting an updated picture of the recent advances made in the biochemical and molecular properties of D1 as well as its role in human physiology PMID: 21415143
    22. lower enzymatic activity in hemangiomas in relation to healthy liver tissue PMID: 20049650
    23. finding that normal pituitary tissue and pituitary adenomas expressed 5'-deiodinase activity implies that both enzymes are active in tumors and local deiodination is important for function and feedback regulation of anterior pituitary PMID: 12153750
    24. overexpressed iodothyronine deiodinase types 1,2, and 3 can homodimerize probably through disulfide bridges and types 1 and 2 monomeric forms are catalytically active PMID: 12586771
    25. D1 contains a glycoside hydrolase clan GH-A-like structure PMID: 12847093
    26. DIO1 and DIO2 were underexpressed in nearly all papillary thyroid carcinomas PMID: 15785240
    27. many factors interact in complex ways to establish D1 levels, contributing to the circulating concentrations of thyroxine (T(4)) and T(3) [review] PMID: 16131326
    28. association of D1a-C/T and D1b-A/G polymorphisms with iodothyronine levels in the elderly PMID: 17105838
    29. presence of type 1 5'-deiodinase in well-differentiated breast cancer tissue PMID: 17274741
    30. The role of type 1 and type 2 5'-deiodinase in the pathophysiology of the 3,5,3'-triiodothyronine toxicosis of McCune-Albright syndrome. PMID: 18349068
    31. HNF4alpha regulates thyroid hormone homeostasis through transcriptional regulation of the Dio1 gene with GATA4 and KLF9 PMID: 18426912
    32. common genetic variation in DIO1 alters deiodinase function, resulting in an alteration in the balance of circulating free T(3) to free T(4). PMID: 18492748
    33. The D1-C785T polymorphism is significantly associated with serum thyroid hormone levels. PMID: 18793344
    34. A functional polymorphism in type 1 deiodinase is associated with enhanced potentiation of the antidepressant effect of sertraline by triiodothyronine PMID: 19064291

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Iodothyronine deiodinase family
  • 数据库链接：

    HGNC: 2883

    OMIM: 147892

    KEGG: hsa:1733

    STRING: 9606.ENSP00000354643

    UniGene: Hs.251415