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  4. Recombinant Human Transient receptor potential cation channel subfamily V member 3 (TRPV3)

Recombinant Human Transient receptor potential cation channel subfamily V member 3 (TRPV3)

In Stock
  • 中文名称：
    人TRPV3重组蛋白
  • 货号：
    CSB-CF854113HU
  • 规格：
    ￥12960
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    TRPV3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    93.1 kDa
  • 表达区域：
    1-790aa
  • 氨基酸序列
    MKAHPKEMVPLMGKRVAAPSGNPAILPEKRPAEITPTKKSAHFFLEIEGFEPNPTVAKTSPPVFSKPMDSNIRQCISGNCDDMDSPQSPQDDVTETPSNPNSPSAQLAKEEQRRKKRRLKKRIFAAVSEGCVEELVELLVELQELCRRRHDEDVPDFLMHKLTASDTGKTCLMKALLNINPNTKEIVRILLAFAEENDILGRFINAEYTEEAYEGQTALNIAIERRQGDIAALLIAAGADVNAHAKGAFFNPKYQHEGFYFGETPLALAACTNQPEIVQLLMEHEQTDITSRDSRGNNILHALVTVAEDFKTQNDFVKRMYDMILLRSGNWELETTRNNDGLTPLQLAAKMGKAEILKYILSREIKEKRLRSLSRKFTDWAYGPVSSSLYDLTNVDTTTDNSVLEITVYNTNIDNRHEMLTLEPLHTLLHMKWKKFAKHMFFLSFCFYFFYNITLTLVSYYRPREEEAIPHPLALTHKMGWLQLLGRMFVLIWAMCISVKEGIAIFLLRPSDLQSILSDAWFHFVFFIQAVLVILSVFLYLFAYKEYLACLVLAMALGWANMLYYTRGFQSMGMYSVMIQKVILHDVLKFLFVYIVFLLGFGVALASLIEKCPKDNKDCSSYGSFSDAVLELFKLTIGLGDLNIQQNSKYPILFLFLLITYVILTFVLLLNMLIALMGETVENVSKESERIWRLQRARTILEFEKMLPEWLRSRFRMGELCKVAEDDFRLCLRINEVKWTEWKTHVSFLNEDPGPVRRTDFNKIQDSSRNNSKTTLNAFEEVEEFPETSV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-Flag-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen).
  • 基因功能参考文献：
    1. Data suggest that Thr264 in TRPV3 is key ERK1 phosphorylation site mediating EGFR-induced sensitization of TRPV3 to stimulate signaling pathways involved in regulating skin homeostasis. (TRPV3 = transient receptor potential cation channel subfamily V member-3; ERK1 = extracellular signal-regulated kinase-1; EGFR = epidermal growth factor receptor) PMID: 29084846
    2. This study identified a direct regulatory effect of MBTPS2 on TRPV3 which can partially contribute to the overlapping clinical features of IFAP and Olmsted syndromes under a common signaling pathway. PMID: 28717930
    3. TRPV3 may contribute to pruritus in burn scars through TSLP. PMID: 29140280
    4. the expression of transient receptor potential vanilloid-1 (TRPV1), transient receptor potential vanilloid-2 (TRPV2) and transient receptor potential vanilloid-3 (TRPV3) channels in native human odontoblasts, was examined. PMID: 28905239
    5. TRPV3 was highly expressed in the infiltrating eosinophils and mucosal epithelium of the nasal polyps of ECRS, and further that the more severe the refractoriness was after surgery, the higher the TRPV3 expression was in nasal polyps. PMID: 28462829
    6. Data indicate that the restoration of a single residue that is apparently missing in the use-dependent homologs could largely eliminate the use dependence of heat sensitivity of vanilloid receptor transient receptor potential 3 (TRPV3). PMID: 28154143
    7. TRPV3 mutants causing Olmsted Syndrome induce impaired cell adhesion and nonfunctional lysosomes PMID: 27754757
    8. *We describe two cases of Olmsted-like syndrome in a Mongolian family. *The underlying cause was a previously undescribed G573V point mutation in TRPV3. PMID: 27273692
    9. The results of the present study show that polymorphism of TRPV3 contributed towards symptom severity in FM. PMID: 27079220
    10. A novel mutation in TRPV3 gene causes atypical familial Olmsted syndrome in a Chinese family. PMID: 26902751
    11. High Transient receptor potential vanilloid 3 protein expression could promote the proliferation of lung cancer cells. Transient receptor potential vanilloid 3 inhibition decreased [Ca2+]i of lung cancer cells and cell cycle arrest at the G1/S boundary. PMID: 27023518
    12. these data suggest that TRPV3 sparklets cause dilation of cerebral parenchymal arterioles by activating IK and SK channels in the endothelium PMID: 26453324
    13. TRPV3 missense mutation identified as a cause of the rare Olmsted syndrome. PMID: 26067147
    14. TPRV3 was significantly elevated in the epidermis of burn scars with pruritus. PMID: 24695993
    15. Study illustrates the antiadipogenic role of TRPV3 in the adipocytes. PMID: 25774551
    16. this study provides powerful tools to broaden our understanding of ligand interaction with TRPV channels, and the availability of purified human TRPV3 opens up perspectives for further structural and functional studies PMID: 25829496
    17. A mutation in TRPV3 causes focal palmoplantar keratoderma in a Chinese family. PMID: 25285920
    18. Demonstrate similarities but also notable differences in TRPV3 pharmacology between recombinant and native systems. PMID: 23848361
    19. a TRPV3 mutation has a role in Olmsted syndrome [case report] PMID: 24463422
    20. TRPV3 missense mutation found in patient with Olmsted syndrome. PMID: 24452206
    21. TRPV3-ARD with characteristic finger 3 loop likely plays an important role in channel function and pharmacology. PMID: 24248473
    22. TRPV3 has roles in skin physiology and in certain skin diseases [review] PMID: 23800054
    23. The Olmsted syndrome patient was found to harbour a previously undescribed 1718G-C transversion in TRPV3, causing a G573A point mutation with immunological dysregulation function. PMID: 23692804
    24. TRPV3 is a therapeutic target for itch PMID: 22475759
    25. Nucleotide sequencing of five additional affected individuals also revealed missense mutations in TRPV3. PMID: 22405088
    26. Nominal association was confirmed for TRPV3 rs7217270 in migraine with aura and TRPV1 rs222741 in the overall migraine group. PMID: 22162417
    27. It was concluded that the sensitization of TRPV3 is intrinsic to the channel itself and occurs as a result of hysteresis of channel gating. PMID: 22006988
    28. TRPV3 channel is expressed in skin, its likely role is to detect noxious cold temperatures. PMID: 21490957
    29. PI(4,5)P(2)-dependent modulation of TRPV3 activity represents an attractive mechanism for acute regulation of keratinocyte signaling cascades that control cell proliferation and the release of autocrine and paracrine factors. PMID: 21321070
    30. Farnesyl pyrophosphate is the firstly identified endogenous TRPV3 activator that causes nociception PMID: 20395302
    31. A multiligand binding site for ATP and calmodulin previously identified in the TRPV1 ankyrin repeat domain is conserved in TRPV3 and TRPV4, but not TRPV2. PMID: 19864432
    32. member of the vanilloid channel family that is expressed in skin, tongue, dorsal root ganglion, trigeminal ganglion, spinal cord and brain; a calcium-permeable temperature-sensitive cation channel PMID: 12077604
    33. temperature-sensitive (but capsaicin-insensitive) vanilloid receptor-like protein; may represent an additional vanilloid receptor subunit involved in the formation of heteromeric vanilloid receptor channels PMID: 12077606
    34. Ca(2+) inhibits TRPV3 from both the extracellular and intracellular sides. The inhibition is sequentially reduced, appearing as sensitization to repetitive stimulations. PMID: 18178557
    35. vanilloid type 3 (TRPV3) channel is crucially involved in pruritic dermatitis [review] PMID: 19209153

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  • 相关疾病：
    Olmsted syndrome (OLMS); Palmoplantar keratoderma, non-epidermolytic, focal 2 (FNEPPK2)
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Transient receptor (TC 1.A.4) family, TrpV subfamily, TRPV3 sub-subfamily
  • 组织特异性：
    Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level). Expressed in the keratinocyte layers of the outer root sheath and, to lesser extent, to the matrix of the hair follicles (at protein level).
  • 数据库链接：

    HGNC: 18084

    OMIM: 607066

    KEGG: hsa:162514

    STRING: 9606.ENSP00000461518

    UniGene: Hs.446255