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  4. Recombinant Human Transcriptional repressor CTCF (CTCF), partial

Recombinant Human Transcriptional repressor CTCF (CTCF), partial

  • 中文名称：
    Recombinant Human Transcriptional repressor CTCF(CTCF),partial
  • 货号：
    CSB-CF006138HU1
  • 规格：
    ￥5028
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    CTCF; Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    57.4kDa
  • 表达区域：
    266-727aa
  • 氨基酸序列
    FQCELCSYTCPRRSNLDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKPFKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSGTMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHKNEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKCGKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSENAEPDLDDNEDEEEPAVEIEPEPEPQPVTPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKEPDAEPAEGEEEEAQPAATDAPNGDLTPEMILSMMDR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    This recombinant HumanCTCF protein is an in vitro E.coli (cell-free) expressed partial protein. Its purity is 90%+ determined by SDS-PAGE. Cell-free protein expression is the in vitro synthesis of a protein using translation-compatible extracts of whole cells. In principle, whole-cell extracts contain all the macromolecules and components needed for transcription, translation, and even post-translational modification. These components include RNA polymerase, regulatory protein factors, transcription factors, ribosomes, and tRNA. When supplemented with cofactors, nucleotides, and the specific gene template, these extracts can synthesize proteins of interest in a few hours.

    CTCF, best known as a transcriptional factor, is a highly conserved multifunctional DNA-binding protein with 11 zinc fingers. It plays indispensable roles in methylation maintenance, transcriptional inhibition/activation, insulation, gene imprinting, and regulation of 3D genome organization. CTCF is responsible for the formation of genome multi-dimensions, regulation of dimensional changes, or control of central signals to transcriptional networks. CTCF has recently been found to participate in the repair of DNA double-strand breaks (DSBs) and in the maintenance of genome fidelity.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis.
  • 基因功能参考文献：
    1. The s propose that cellular CCCTC-binding factor binding at the herpes simplex virus 1 CCCTC-binding factor binding sites (CTRL2) acts as a chromatin insulator to keep viral chromatin in a form that is poised for reactivation, a state which we call poised latency. PMID: 29437926
    2. neither the deletion of the CTCF locus nor the ectopic insertion of Firre cDNA or its ectopic expression are sufficient to alter topologically associated domains in a sex-specific or allele-specific manner PMID: 29654311
    3. CTCF maintains regulatory homeostasis of cancer pathways. PMID: 30086769
    4. these results suggest that CTCF participate in DNA damage response via poly(ADP-ribosylation). PMID: 28262757
    5. Studies suggest that the connection between DNA-binding protein CTCF (CTCF), cohesin, chromatin structure, and behavior is important in understanding of the development of behavior in general, and neurodevelopmental disorders in particular [Review]. PMID: 29110030
    6. ID1, CTCF and ELK1 may be associated with prostate cancer, and may be potential therapeutic targets for the treatment of this disease. PMID: 29956775
    7. CTCF promotes HSV-1 lytic transcription by facilitating the elongation of RNA Pol II and preventing silenced chromatin on the viral genome. PMID: 28045091
    8. These results, together with a prior exomesequencing based study, suggest that CTCF mutations may be involved in the development of ovarian endometriosis. PMID: 29845264
    9. findings establish for the first time that CTCF is an important regulator of the homologous recombination repair pathway. PMID: 28560323
    10. Findings indicate that CCCTC-binding factor (CTCF)-driven doublesex and mab-3 related transcription factor 2 protein (TERRA) transcription acts in cis to facilitate telomere repeat replication and chromosome stability. PMID: 29235471
    11. poly(ADP-ribosyl)ated CTCF changes its DNA binding and localisation in a breast cell line which is associated with nucleosome repositioning. PMID: 29981477
    12. Here, we show that PARP1 and host insulator protein CTCF colocalize at specific sites throughout the EBV genome and provide evidence to suggest that PARP1 acts to stabilize CTCF binding and maintain the open chromatin landscape at the active Cp promoter during type III latency. Further, PARP1 activity is important in maintaining latency type-specific viral gene expression. PMID: 29976663
    13. HOTTIP cooperates with CTCF to coordinate HOXA gene expression. PMID: 29698677
    14. CD4(+) T cells showed the greatest increase (threefold) in ORMDL3 expression in individuals carrying the asthma-risk alleles, where ORMDL3 negatively regulated interleukin-2 production. The asthma-risk variants rs4065275 and rs12936231 switched CTCF-binding sites in the 17q21 locus. PMID: 27848966
    15. Our data reveal that vigilin is essential for maintenance of imprinting of IGF2 gene via functional interaction between KH1-7 domains of vigilin and zinc-finger domains of CTCF. PMID: 29157910
    16. This study confirms that haploinsufficiency of CTCF causes distinct clinical features, and that a microdeletion encompassing CTCF could cause a recognisable CTCF deletion syndrome. Perturbed DNA methylation at CTCF binding sites, not at imprinted loci, may underlie the pathomechanism of the syndrome. PMID: 28848059
    17. structural studies show that the sequence-specific interactions between zinc fingers and CTCF-binding sites determine the directionality and conservation of CTCF recognition. PMID: 29076501
    18. CTCF may be a key factor that contributes to gene co-mutations in cancer. PMID: 27762310
    19. results support a model in which YY1 acts as an architectural protein to connect developmentally regulated looping interactions; the location of YY1-mediated interactions may be demarcated in development by a preexisting topological framework created by constitutive CTCF-mediated interactions. PMID: 28536180
    20. The MeCP2, a protein whose mutated forms are involved in Rett syndrome; and CTCF, a constitutive transcriptional insulator. PMID: 28796949
    21. The results show that cohesin has an essential genome-wide function in mediating long-range chromatin interactions and support the hypothesis that cohesin creates these by loop extrusion, until it is delayed by CTCF in a manner dependent on PDS5 proteins, or until it is released from DNA by WAPL. PMID: 29217591
    22. CTCF-FOXM1 axis regulates tumour growth and metastasis in hepatocellular carcinoma cells. PMID: 28862757
    23. we show CTCF binding site mutations to be functional by demonstrating allele-specific reduction of CTCF binding to mutant alleles. While topologically associating domains with mutated CTCF anchors in melanoma contain differentially expressed cancer-associated genes, CTCF motif mutations appear generally under neutral selection PMID: 27974201
    24. CTCF-mediated long-range interactions are integral for a multitude of topological features of interphase chromatin, such as the formation of topologically associated domains, domain insulation, enhancer blocking and even enhancer function. PMID: 26802288
    25. s have identified two novel pro-tumorigenic roles (promoting cell survival and altering cell polarity) for genetic alterations of CTCF in endometrial cancer. PMID: 28319062
    26. Describe several protein-DNA complex structures of a human CTCF tandem zinc-finger array, explaining the adaptability of CTCF to sequence variations and the positiondependent effect of differential DNA methylation at two cytosine residues, and revealing a potential function of C-terminal ZF8 and ZF9 spanning across the DNA phosphate backbone. PMID: 28529057
    27. CCCTC-binding factor (CTCF) targets the binding sites within MYCN promoter to facilitate its expression in neuroblastoma (NB) cells. PMID: 26549029
    28. we review recent high-resolution chromosome conformation capture and functional studies that have informed models of the spatial and regulatory compartmentalization of mammalian genomes, and discuss mechanistic models for how CTCF and cohesin control the functional architecture of mammalian chromosomes. PMID: 27089971
    29. GAD1 is reactivated by DNA methylation, which provided a model for DNA methylation and the active orchestration of oncogenic gene expression by CTCF in cancer cells. PMID: 26549033
    30. epigenetic factor CTCF-mediated chromatin remodeling regulates interactions between eye-specific PAX6 and those genes that are induced/associated with cell differentiation to modulate corneal epithelial cell-specific differentiation PMID: 27583466
    31. Rta-mediated decreased binding of CTCF in the viral genome is concurrent with virus reactivation. Via interfering with CTCF binding, in the host genome Rta can function as a transcriptional repressor for gene silencing. PMID: 28490592
    32. although we were unable to detect HD-associated DNA methylation alterations at queried sites, we found that DNA methylation may be correlated to the age of disease onset in cortex tissues. Moreover, our data suggest that DNA methylation may, in part, contribute to tissue-specific HTT transcription through differential CTCF occupancy. PMID: 26953320
    33. vitamin D-sensitive CTCF sites provide further mechanistic details to the epigenome-wide understanding of 1,25(OH)2D3-mediated gene regulation PMID: 27569350
    34. These findings indicate that erythroid specific activator GATA-1 acts at CTCF sites around the beta-globin locus to establish tissue-specific chromatin organization. PMID: 28161276
    35. TOP2B is positioned to solve topological problems at diverse cis-regulatory elements and its occupancy is a highly ordered and prevalent feature of CTCF/cohesin binding sites that flank TADs. PMID: 27582050
    36. CTCF binding to eRNAs and promoters is facilitated by estrogen when chromatin establishes contacts with nuclear lamina. PMID: 27638884
    37. we investigated the cell-type specificities of CTCF sites related to these functions across five cell types. Our study provides new insights into the multivalent functions of CTCF in the human genome PMID: 27067545
    38. The results indicate that the initial chromatin conformation affects subsequent RA-induced HOXA gene activation. Our study uncovers that a removable insulator spatiotemporally switches higher-order chromatin and multiple gene activities via cooperation of CTCF and key transcription factors. PMID: 27798106
    39. we find no evidence for selection driving these distinctive patterns of mutation. The mutational load at CTCF-binding sites is substantially determined by replication timing and the mutational signature of the tumor in question, suggesting that selectively neutral processes underlie the unusual mutation patterns. PMID: 27490693
    40. data demonstrate a reciprocal relationship between Hmgb2 and Ctcf in controlling aspects of chromatin structure and gene expression. PMID: 27226577
    41. Study described the formation of mutually exclusive complexes of ENY2 with insulator proteins and Sgfl1-a component of the SAGA complex, direct binding partner for ENY2 PMID: 27417714
    42. This is supported by the depletion of CTCF in glioblastoma cells affecting the expression levels of NOTCH2 as a target of miR-181c. CONCLUSION: Together, our results point to the epigenetic role of CTCF in the regulation of microRNAs implicated in tumorigenesis PMID: 26983574
    43. Our data show that aberrant epigenetic inactivation of DUSP2 occurs in carcinogenesis and that CTCF is involved in the epigenetic regulation of DUSP2 expression. PMID: 26833217
    44. Mutational analysis highlighted a potential role for CTCF, a crucial regulator of long-range chromatin interactions, in head and neck cancer progression. PMID: 26747525
    45. CTCF and cohesins shape the genome during evolution. (Review) PMID: 26439501
    46. The action of SNF2H at CTCF sites is functionally important as depletion of CTCF or SNF2H affects transcription of a common group of genes. PMID: 27019336
    47. CSB and CTCF can regulate each other's chromatin association, thereby modulating chromatin structure and coordinating gene expression in response to oxidative stress. PMID: 26578602
    48. CTCF binds to the provirus at a sharp border in epigenetic modifications in the pX region of the HTLV-1 provirus in T cells naturally infected with HTLV-1. This may cause widespread abnormalities in host cell chromatin structure and gene expression. PMID: 26929370
    49. CTCF/cohesin coordinates HOXA cluster higher-order chromatin structure and expression during development PMID: 26376810
    50. CTCF has a role in regulating SLC45A3-ELK4 Chimeric RNA PMID: 26938874

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  • 相关疾病：
    Mental retardation, autosomal dominant 21 (MRD21)
  • 亚细胞定位：
    Nucleus, nucleoplasm. Chromosome. Chromosome, centromere. Note=May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.
  • 蛋白家族：
    CTCF zinc-finger protein family
  • 组织特异性：
    Ubiquitous. Absent in primary spermatocytes.
  • 数据库链接：

    HGNC: 13723

    OMIM: 604167

    KEGG: hsa:10664

    STRING: 9606.ENSP00000264010

    UniGene: Hs.368367