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  4. Recombinant Human TIR domain-containing adapter molecule 1 (TICAM1)

Recombinant Human TIR domain-containing adapter molecule 1 (TICAM1)

  • 货号：
    CSB-CF811598HUe1
  • 规格：
    ￥6864
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    TICAM1; PRVTIRB; TRIF; TIR domain-containing adapter molecule 1; TICAM-1; Proline-rich, vinculin and TIR domain-containing protein B; Putative NF-kappa-B-activating protein 502H; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta; MyD88-3; TIR domain-containing adapter protein inducing IFN-beta
  • 种属：
    Homo sapiens(Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    76.4kDa
  • 表达区域：
    1-712aa
  • 氨基酸序列
    MACTGPSLPSAFDILGAAGQDKLLYLKHKLKTPRPGCQGQDLLHAMVLLKLGQETEARISLEALKADAVARLVARQWAGVDSTEDPEEPPDVSWAVARLYHLLAEEKLCPASLRDVAYQEAVRTLSSRDDHRLGELQDEARNRCGWDIAGDPGSIRTLQSNLGCLPPSSALPSGTRSLPRPIDGVSDWSQGCSLRSTGSPASLASNLEISQSPTMPFLSLHRSPHGPSKLCDDPQASLVPEPVPGGCQEPEEMSWPPSGEIASPPELPSSPPPGLPEVAPDATSTGLPDTPAAPETSTNYPVECTEGSAGPQSLPLPILEPVKNPCSVKDQTPLQLSVEDTTSPNTKPCPPTPTTPETSPPPPPPPPSSTPCSAHLTPSSLFPSSLESSSEQKFYNFVILHARADEHIALRVREKLEALGVPDGATFCEDFQVPGRGELSCLQDAIDHSAFIILLLTSNFDCRLSLHQVNQAMMSNLTRQGSPDCVIPFLPLESSPAQLSSDTASLLSGLVRLDEHSQIFARKVANTFKPHRLQARKAMWRKEQDTRALREQSQHLDGERMQAAALNAAYSAYLQSYLSYQAQMEQLQVAFGSHMSFGTGAPYGARMPFGGQVPLGAPPPFPTWPGCPQPPPLHAWQAGTPPPPSPQPAAFPQSLPFPQSPAFPTASPAPPQSPGLQPLIIHHAQMVQLGLNNHMWNQRGSQAPEDKTQEAE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    NO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines.
  • 基因功能参考文献：
    1. These data indicate a novel role of the TLR3-TICAM-1 pathway in controlling miR-21 levels in extracellular vesicles. PMID: 29679565
    2. Findings suggest that Toll/IL-1R domain-containing adapter-inducing IFN-beta may be involved in the epileptogenesis of temporal lobe epilepsy, which would make it a potential therapeutic target for the treatment of epilepsy. PMID: 28867282
    3. Data suggest that endosomal localization of TICAM2 is essential for TLR4-mediated type I interferon-inducing signaling from endosomes; TICAM2 acts as scaffold protein and activates TICAM1; N-terminal myristoylation allows TICAM2 to anchor to endosomal membrane. (TICAM2 = toll like receptor adaptor molecule-2; TICAM1 = toll like receptor adaptor molecule-1; TLR4 = toll-like receptor 4) [REVIEW] PMID: 28630139
    4. Data suggest that pro-death signals through TIR-domain-containing adapter-inducing interferon-beta (TRIF) are regulated by autophagy and propose that pro-apoptotic signalling through TRIF/RIPK1/caspase-8 occurs in fibrillary platforms. PMID: 28453927
    5. TRIF gene may contribute to susceptibility of T2DM. PMID: 28479387
    6. The NF-kappaB activated by dsRNA appears not to be the canonical p65/p50 heterodimers. PMID: 28052863
    7. IL-12p70 production requires uptake of Streptococcus pneumoniae as well as the presence of the adaptor molecule TRIF. PMID: 26956584
    8. TIRF high-content imaging system simultaneously showed the expression pattern of EGFRs and EC50 value for CPT-induced apoptosis and necrosis in MCF-7, SK-BR-3 and JIMT-1 cancer cell line PMID: 26886741
    9. involved in signaling that moderates the expression of heme oxygenase1 and il-8 after keratinocytes exposure to dinitrochlorobenzene PMID: 25377948
    10. signaling through TRIF is important for the inflammatory response of AngII-induced abdominal aortic aneurysm formation PMID: 26100679
    11. Data show that toll-like receptor 3/TRIF protein signalling regulates cytokines IL-32 and IFN-beta secretion by activation of receptor-interacting protein-1 (RIP-1) and tumour necrosis factor receptor-associated factor 6 (TRAF6) in cornea epithelial cells. PMID: 25754842
    12. In mice with nonfunctional TRIF (Trif(mut) mice), Ang II-induced hypertension and cardiac hypertrophy were abrogated, and proinflammatory gene expression in heart and kidneys was unchanged or decreased. These results indicate that Ang II induces activation of a proinflammatory innate immune response, causing hypertension and cardiac hypertrophy, which require functional adaptor protein TRIF-mediated pathways. PMID: 26195481
    13. TRIF promotes angiotensin II-induced cross-talk between fibroblasts and macrophages in atrial fibrosis. PMID: 26056004
    14. amino-terminal 21 amino acid sequence is crucial for TRAF3 association, protein stability and activation of the IFN pathway PMID: 24577058
    15. required, in combination with MyD88, for TLR4-mediated proinflammatory dendritic cell differentiation PMID: 23941760
    16. findings show that TRIF activates IRF3 through a phosphorylation-dependent mechanism; results reveal that phosphorylation of innate adaptor proteins is an essential and conserved mechanism that selectively recruits IRF3 to activate the type I IFN pathway PMID: 25636800
    17. PTX3 binds MD-2 and promotes TRIF-dependent immune protection in aspergillosis. PMID: 25049357
    18. MUC1 has a role in regulating epithelial inflammation and apoptosis by PolyI:C through inhibition of TRIF recruitment to Toll-like receptor 3 PMID: 24693944
    19. Enterovirus 68 3C protease cleaves TRIF to attenuate antiviral responses mediated by TLR3. PMID: 24672048
    20. TRIF-N-terminal helical domain shares structural similarity with IFIT5. PMID: 24311583
    21. IL-32-PAR2 axis is an innate immunity sensor providing alternative signaling for LPS-TRIF axis. PMID: 24129891
    22. required for HLA class I antigen presentation during Hantavirus infection PMID: 23824566
    23. Data indicate that TIR domain containing adaptor inducing IFN-beta protein (TRIF), as well as TLR3 activation, enhances Kaposi's sarcoma-associated herpesvirus (KSHV) replication and transcription activator (RTA) protein expression. PMID: 23723066
    24. TRIM38 may act as a novel negative regulator for TLR3-mediated type I interferon signaling by targeting TRIF for degradation PMID: 23056470
    25. ADAM15 acts as a negative regulator of TRIF-mediated NF-kappaB and IFN-beta reporter gene activity via TLR3 and TLR4 signaling. PMID: 23365087
    26. anti-beta(2)GPI/beta(2)GPI complex induced TF and TNF-alpha expression involving both TLR4/MyD88 and TLR4/TRIF signaling pathways and TLR4 and its adaptors might be molecular targets for therapy of antiphospholipid syndrome PMID: 22964479
    27. TICAM1 down-regulation was found to be dependent on TLR3 but not RIG1, MDA5, or PKR and appeared to be regulated post-translationally PMID: 22205631
    28. Data show that The TRIF-selective signaling response resulted in the production of substantially less of the proinflammatory mediators. PMID: 22337809
    29. the D299G polymorphism compromises recruitment of MyD88 and TRIF to TLR4 without affecting TLR4 expression, TLR4-MD2 interaction, or LPS binding, suggesting that it interferes with TLR4 dimerization PMID: 22474023
    30. TRIF may play an important role in the pathogenesis of antiphospholipid syndrome. PMID: 22152804
    31. regulation of the TLR3/TRIF-mediated pathway required the combined action of SHP-1 and SHP-2, which could be accomplished by CD300f but not CD300a PMID: 22043923
    32. The TLR2/4 ligand biglycan enhances antigen-specific T cell priming via MyD88 and Toll-like receptor adapter molecule TRIF pathways, triggering autoimmune perimyocarditis in transgenic mice. PMID: 22095710
    33. Hepatitis A virus inhibits TLR3 signaling by reducing abundance of the adaptor protein TRIF. PMID: 21931545
    34. Susceptibility to herpes simplex encephalitis is caused by TRIF nonsense (autosomal recessive) TRIF deficiency or a missense mutation (autosomal dominant). TRIf is needed for TLR3-dependent interferon synthesis. PMID: 22105173
    35. The host TICAM-1 transgene pathway is essential for antiviral responses that suppress poliovirus infection, which is exacerbated in TICAM-1-deficient poliovirus receptor-bearing mice. PMID: 21998457
    36. show that hBD3 reduces NF-kappaB signaling in cells transfected with MyD88 or TRIF PMID: 21809339
    37. These studies establish that MyD88 but not Trif signaling plays a critical role in mediating cardiac dysfunction, systemic inflammation, and mortality during polymicrobial sepsis PMID: 21792053
    38. PLIC-1 is a novel inhibitor of the TLR3-Trif antiviral pathway by reducing the abundance of Trif PMID: 21695056
    39. TRIF cleavage mediated by human enterovirus 71 3C protease may be a mechanism to impair type I interferon production in response to Toll-like receptor 3 (TLR3) activation. PMID: 21697485
    40. KSHV employs a novel mechanism to block the innate immunity by degrading TRIF protein. PMID: 21212282
    41. TRIF protein induces soluble tumor necrosis factor receptor 1 (TNFR1) shedding via signaling from human airway epithelial cells (NCI-H292) with subsequent activation of two downstream pathways. PMID: 21148036
    42. Silencing TRIF expression reduces TLR5-induced NFkappaB, JNK1/2, and ERK1/2 activation in human colonic epithelial cells. PMID: 20855887
    43. Data show that mutant TICAM-1 failed to recruit the IRF-3 kinase TBK1, resulting in lack of IRF-3 phosphorylation. PMID: 20418377
    44. TRIF modulates TLR5-dependent responses by inducing proteolytic degradation of TLR5 PMID: 20452988
    45. Consistent with results for total tumor necrosis factor (TNF)-alpha messenger RNA (mRNA) levels, lipopolysaccharide-stimulated Trif transgenic and wild-type bone marrow-derived dendritic cells generate comparable amounts of TNF-alpha transcripts. PMID: 20375303
    46. SARM-mediated inhibition may not be exclusively directed at TRIF or MyD88, but that SARM may also directly inhibit MAPK phosphorylation PMID: 20306472
    47. TRIS was associated with TRIF upon TLR3 activation by poly(I-C). These findings reveal an unexpected mechanism of TLR3-mediated signaling. PMID: 20200155
    48. The binding of TRAF2 and TRAF6 to TICAM-1 cooperatively activates the IFN-inducing pathway through ubiquitination of TICAM-1. PMID: 20047764
    49. TRIF is identified and characterized as a novel adaptor protein containing the Toll/IL-1 receptor domain, with a specific role in TLR3 signaling. PMID: 12471095
    50. identified an alternative adaptor, designated Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule (TICAM)-1, that can physically bind the TIR domain of TLR3 PMID: 12539043

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  • 相关疾病：
    Herpes simplex encephalitis 4 (HSE4)
  • 亚细胞定位：
    Cytoplasmic vesicle, autophagosome. Cytoplasm, cytosol. Mitochondrion.
  • 组织特异性：
    Ubiquitously expressed but with higher levels in liver.
  • 数据库链接：

    HGNC: 18348

    OMIM: 607601

    KEGG: hsa:148022

    STRING: 9606.ENSP00000248244

    UniGene: Hs.29344