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  4. Recombinant Human Delta-type opioid receptor (OPRD1)

Recombinant Human Delta-type opioid receptor (OPRD1)

In Stock
  • 货号：
    CSB-CF016358HU
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    41.9 kDa
  • 表达区域：
    1-372aa
  • 氨基酸序列
    MEPAPSAGAELQPPLFANASDAYPSACPSAGANASGPPGARSASSLALAIAITALYSAVCAVGLLGNVLVMFGIVRYTKMKTATNIYIFNLALADALATSTLPFQSAKYLMETWPFGELLCKAVLSIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPAKAKLINICIWVLASGVGVPIMVMAVTRPRDGAVVCMLQFPSPSWYWDTVTKICVFLFAFVVPILIITVCYGLMLLRLRSVRLLSGSKEKDRSLRRITRMVLVVVGAFVVCWAPIHIFVIVWTLVDIDRRDPLVVAALHLCIALGYANSSLNPVLYAFLDENFKRCFRQLCRKPCGRPDPSSFSRAREATARERVTACTPSDGPGGGAAA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.
  • 基因功能参考文献：
    1. During prolonged hypoxia/ischemia delta-opioid receptor (DOR) neuroprotection involves a variety of signaling pathways. The data suggest that DOR may display its neuroprotective role via the BDNF-TrkB pathway. [review] PMID: 29768254
    2. Site-specific O-glycosylation of N-terminal serine residues by polypeptide GalNAc-transferase 2 modulates human delta-opioid receptor turnover at the plasma membrane. PMID: 29097258
    3. OPRD1 gene variants and their haplotypes can play important roles in susceptibility to opioid dependence. PMID: 28632076
    4. Homozygotes AA at rs6265 (BDNF), TT at rs16917234 (BDNF), and CC at rs508448 (OPRD1) also appeared as risk factors for the endophenotype earlier age of onset for heroin use. PMID: 28692418
    5. Di-lysine binding motifs within the second and third intracellular loops of DOPr are involved in the interaction with COPI. PMID: 28041939
    6. Functional activity of Dynorphin 1-17 and fragments (1-6, 1-7 and 1-9) were screened over a range of concentrations against forskolin stimulated human embryonic kidney 293 (HEK) cells stably transfected with one of KOP, MOP or DOP PMID: 28049031
    7. A polymorphism in OPRD1 appears to be associated with both cocaine dependence and cocaine use during treatment in African-Americans. Follow-up studies to confirm the effect on cocaine use are warranted. PMID: 27449273
    8. OPRD1 promoter hypermethylation is associated with the risk of AD PMID: 28253273
    9. This review addresses Delta Opioid receptors from the perspective of cellular and molecular determinants of their pharmacological diversity. PMID: 27343248
    10. After inhibition of the PKC/ERK signalling pathway, the effects of DOR on breast cancer were significantly attenuated in vivo and in vitro. In summary, DOR is highly expressed in breast cancer and is closely related to its progression. These results suggest that DOR may serve as a potential biomarker for the early diagnosis of breast cancer and may be a viable molecular target for therapeutic intervention. PMID: 27665747
    11. The study successfully developed a population PKPD model for oxycodone across three experimental pain types and investigated the association between 18 SNPs across all three opioid receptor genes (OPRM1, OPRK1, OPRD1) and analgesic response from oxycodone. None of the selected polymorphisms were significantly associated with analgesic response of oxycodone. PMID: 26946441
    12. the expression of DOR was upregulated in the drugresistant human hepatocellular carcinoma cells. PMID: 26549838
    13. OPRD-mediated activation of ERK1/2 is via ligand-specific transactivation of EGFR. PMID: 26211551
    14. Results suggest deltaOR-Phe27Cys variation modulates beta- and gamma-secretase activity in late-stage Alzheimer's disease likely via post-translational mechanisms PMID: 26402014
    15. findings suggest that SNPs in opioid receptors and the PNOC genes are associated with NAS severity. PMID: 26233486
    16. Activation of the delta opioid receptor promotes cutaneous wound healing by affecting keratinocyte intercellular adhesion and migration PMID: 24628261
    17. Analysis of the transcription factor POU2F3 revealed a DOPr-mediated extracellular signal-regulated kinase (ERK)-dependent downregulation of this factorThe delta-opioid receptor affects epidermal homeostasis PMID: 25178105
    18. findings suggest that rs581111 and rs52920 may be useful when considering treatment options for female opioid addicts, however, confirmation in an independent sample is warranted PMID: 24126707
    19. OPRD1 rs678849 polymorphism was associated with reduced brain volumes in young participants and elderly Alzheimer's Disease and Mild Cognitive Impairment patients, but brain regions affected differed PMID: 23427138
    20. Taken together, the hdeltaOR appears to rely primarily on the CNX-mediated N-glycan-dependent QC that has the capacity to assist in folding PMID: 24798333
    21. OPRD1 polymorphisms are associated with risk for heroin dependence. PMID: 22500942
    22. Some variants less known for obesity-susceptibility such as OPRD1 were found to be associated to weight with strongest effects in males. PMID: 23318717
    23. DOR is highly expressed in HCC and is involved in HCC progression, suggesting that DOR is a potential target for HCC treatment PMID: 23903826
    24. crystal structure of the human delta-opioid receptor (delta-OR), revealing the presence and fundamental role of a sodium ion in mediating allosteric control of receptor functional selectivity and constitutive activity PMID: 24413399
    25. The levels of spontaneous and ligand-induced receptor-G protein coupling in delta (DOP) and mu (MOP) opioid receptors, was examined. PMID: 23836900
    26. Polymorphisms in OPRD1 are relevant for cocaine addiction in the African American population and support a broad role for OPRD1 variants in drug dependence. PMID: 22795689
    27. delta and mu-opioid receptors, but not kappa-opioid receptors, are functional in the neuronally stimulated longitudinal human vas deferens PMID: 22752269
    28. SNPs rs569356 in OPRD1 (variant A1968G in the promoter region) was not associated with respiratory syncytial virus infection PMID: 23222260
    29. no differences in DOR mRNA levels were seen in schizophrenia. PMID: 21810780
    30. between CBR and delta opioid receptor PMID: 22235275
    31. ssarrestin1 is exclusively involved in human delta-opioid receptor desensitization PMID: 22101011
    32. Cys-27 variant of human delta-opioid receptor modulates maturation and cell surface delivery of Phe-27 variant via heteromerization. PMID: 22184124
    33. study confirmed that common single-nucleotide polymorphisms (SNPs) within OPRD1 confer risk for AN. PMID: 21079607
    34. suggest that aging individuals with at least one human delta opioid receptor(hdeltaOR)(Cys27) encoding allele might have lowered threshold for Ca(2)+ dysregulation in neurons expressing hdeltaOR PMID: 21234650
    35. Data suggest that muOR exists primarily as a dimer that will oligomerize with deltaOR into tetramers, and morphine promotes the dissociation of these tetramers. PMID: 21361347
    36. data suggest that an increased constitutive internalization and/or concurrent signaling of the delta-opioid receptor-Cys27 variant affects APP processing through altered endocytic trafficking of APP PMID: 21464208
    37. Results suggest that the minor G-allele of SNP rs569356 may enhance transcription factor binding and increase OPRD1 expression. PMID: 20300121
    38. human delta opioid receptor (hdeltaOR) exists in a ternary complex with SERCA2b and the ER molecular chaperone calnexin PMID: 20528919
    39. A molecular mechanism for the formation of a DOR/secretase complex that regulates the specificity of secretase for Amyloid beta production. PMID: 20066010
    40. Intrinsic cardiac adrenergic cells constitute a delta-opioid-regulated adrenopeptidergic paracrine system conferring robust cardioprotection through beta(2)-AR/CGRP-R co-signalling. PMID: 19581316
    41. These results suggest that oligomerization of chemokine receptor CCR5 and opioid receptors mu, delta and kappa on the cell membrane of human or monkey lymphocytes may modulate receptor functions. PMID: 12413885
    42. Gene may be linked to etiology of anorexia nervosa. PMID: 12740597
    43. random mutagenesis identified 30 activating point mutations. 3-D modeling revealed an activation path originating from the third extracellular loop and propagating through tightly packed helices III, VI and VII down to a VI-VII cytoplasmic switch. PMID: 12847517
    44. protein conformation and binding site for deltorphin ii PMID: 14999000
    45. interaction studies between the delta-opioid receptor, ligands, and G-proteins PMID: 15317820
    46. investigation of the ability of different opioid receptors to regulate the phosphorylation and degradation of tuberin PMID: 16053916
    47. Results describe the residues of the transmembrane helices 7 of delta and kappa opioid receptors that are on the water-accessible surface of the binding-site crevices. PMID: 16331961
    48. Results suggest that the OPRD1 gene variants may not be a factor in vulnerability to methamphetamine dependence/psychosis. PMID: 16741914
    49. Changes in the inhibitory effects of opioid receptor agonists on intracellular cAMP were used as a marker of the function delta opioid receptors (delta receptor). PMID: 16808998
    50. pharmacological chaperones facilitate plasma membrane targeting of delta-opioid receptors by binding and stabilizing receptor precursors, thereby promoting their release from the stringent ER quality control PMID: 17550902

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes.
  • 数据库链接：

    HGNC: 8153

    OMIM: 165195

    KEGG: hsa:4985

    STRING: 9606.ENSP00000234961

    UniGene: Hs.372