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  4. Recombinant Escherichia coli Peptidyl-prolyl cis-trans isomerase D (ppiD)

Recombinant Escherichia coli Peptidyl-prolyl cis-trans isomerase D (ppiD)

  • 中文名称：
    大肠杆菌ppiD重组蛋白
  • 货号：
    CSB-CF018474ENV
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    ppiD; ybaU; b0441; JW0431; Periplasmic chaperone PpiD; Periplasmic folding chaperone
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-623
  • 氨基酸序列
    MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAKVNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLDQYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQLTTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYYEQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQTKTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKEKGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVSDAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPGINSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLVDLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGNVVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Chaperone that functions as a gatekeeper on the periplasmic side of the SecYEG translocon. Facilitates the translocation of precursor proteins across SecYEG by interacting with the translocating substrate. Also plays a role in the release of newly synthesized secreted proteins at the periplasmic exit site of the Sec translocon. May be involved in the early periplasmic folding of many newly translocated proteins. Acts as a transient subunit of the Sec translocon that binds to the lateral gate of SecY and is detached by nascent membrane proteins but not by SecA.; Does not have peptidyl-prolyl isomerase (PPIase) activity. PPIase activity could not be generated by substitutions at the peptide binding site of the isolated parvulin-like domain.; (Microbial infection) May play an important role in bacteriophage T4 infection and be involved in the penetration of the inner membrane by the bacteriophage injection machinery, resulting in a DNA-conducting channel to translocate the phage DNA into the interior of the cell.
  • 基因功能参考文献：
    1. PpiD contributes to the efficient detachment of newly secreted OmpA from the inner membrane and in doing so, seems to cooperate in a hierarchical manner with other periplasmic chaperones such as SurA, DegP, and Skp. PMID: 29097228
    2. PpiD functions as a chaperone and contributes to the network of periplasmic chaperone activities PMID: 20920237
    3. although PpiD and SurA have partially overlapping substrate specificities, they fulfil different functions in the cell PMID: 18498364
  • 亚细胞定位：
    Cell inner membrane; Single-pass type II membrane protein; Periplasmic side.
  • 数据库链接：

    KEGG: ecj:JW0431

    STRING: 316385.ECDH10B_0397