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  4. Recombinant Drosophila melanogaster Tafazzin homolog (Taz)

Recombinant Drosophila melanogaster Tafazzin homolog (Taz)

  • 中文名称：
    黑腹果蝇Taz重组蛋白
  • 货号：
    CSB-CF893713DLU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Taz
  • Uniprot No.：
  • 别名：
    Taz; CG8766; Tafazzin; TAZ; dTAZ
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-378
  • 氨基酸序列
    MFMVVCSNLRRPGHVGAASAARNINWLISEGYTPPIRAMARPYVQAPEARPVPDERYPGS QQDRKDIATQTVRSSKPKDLRPPSPPTPSQTLNSSSLPPPMSDQDADPSLDVPTGVAMPY NIDWIFPRLRNPSKFWYVVSQFVVSAVGIFSKVVLMFLNKPRVYNRERLIQLITKRPKGI PLVTVSNHYSCFDDPGLWGCLPLGIVCNTYKIRWSMAAHDICFTNKLHSLFFMFGKCIPV VRGIGVYQDAINLCIEKAALGHWIHVFPEGKVNMDKEELRLKWGVGRIIYESPKIPIILP MWHEGMDDLLPNVEPYVIQRGKQVTLNVGQPLDLNDFILDLKKRQVPEPTARKLITDKIQ EAFRDLRAETEKLHRERN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acyltransferase required to remodel newly synthesized phospholipid cardiolipin (1',3'-bis-[1,2-diacyl-sn-glycero-3-phospho]-glycerol or CL), a key component of the mitochondrial inner membrane, with tissue specific acyl chains necessary for adequate mitochondrial function. Remodeling of the acyl groups of CL in the mitochondrial inner membrane affects the assembly and stability of respiratory chain complex IV and its supercomplex forms. This makes CL critical for the coassembly of lipids and proteins in mitochondrial membranes. Catalyzes the transacylacion between phospholipids and lysophospholipids, with the highest rate being between phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) and CL. Catalyzes both 1-acyl-sn-glycero-3-phosphocholine (lysophosphatidylcholine or LPC) reacylation and PC-CL transacylation, that means, it exchanges acyl groups between CL and PC by a combination of forward and reverse transacylations. Also catalyzes transacylations between other phospholipids such as phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) and CL, between PC and PE, and between PC and phosphatidate (1,2-diacyl-sn-glycero-3-phosphate or PA), although at lower rate. Not regiospecific, it transfers acyl groups into any of the sn-1 and sn-2 positions of the monolysocardiolipin (MLCL), which is an important prerequisite for uniformity and symmetry in CL acyl distribution. Cannot transacylate dilysocardiolipin (DLCL), thus, the role of MLCL is limited to that of an acyl acceptor. CoA-independent, it can reshuffle molecular species within a single phospholipid class. Redistributes fatty acids between MLCL, CL, and other lipids, which prolongs the half-life of CL. Its action is completely reversible, which allows for cyclic changes, such as fission and fusion or bending and flattening of the membrane. Hence, by contributing to the flexibility of the lipid composition, it plays an important role in the dynamics of mitochondria membranes. Essential for the final stage of spermatogenesis, spermatid individualization. Required for the initiation of mitophagy.
  • 基因功能参考文献：
    1. propose that tafazzin reacts with non-bilayer-type lipid domains that occur in curved or hemifused membrane zones and that acyl specificity is driven by the packing properties of these domains PMID: 22941046
    2. Data show that tafazzin mutations in Drosophila generate a Barth-related phenotype, with the triad of abnormal cardiolipin, pathologic mitochondria, and motor weakness, suggesting causal links between these findings. PMID: 16855048
    3. Drosophila tafazzin is a CoA-independent, acyl-specific phospholipid transacylase with substrate preference for cardiolipin and phosphatidylcholine PMID: 17082194
    4. The data suggest that tafazzin deficiency affects cardiolipin in all mitochondria, but significant alterations of the ultrastructure, such as remodeling and aggregation of inner membranes, will only occur after specific differentiation. PMID: 19114128
    5. tafazzin deficiency in Drosophila disrupts the final stage of spermatogenesis, spermatid individualization, and causes male sterility PMID: 19164547
    6. the characteristic fatty acid profile of cardiolipin is not determined by the substrate specificity of tafazzin PMID: 19700766

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  • 亚细胞定位：
    Mitochondrion outer membrane; Peripheral membrane protein; Intermembrane side. Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Mitochondrion.; [Isoform A]: Mitochondrion membrane.; [Isoform B]: Mitochondrion membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane.; [Isoform C]: Mitochondrion membrane.
  • 蛋白家族：
    Taffazin family
  • 数据库链接：

    KEGG: dme:Dmel_CG8766

    STRING: 7227.FBpp0086975

    UniGene: Dm.6762