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  4. Recombinant Drosophila melanogaster Atlastin (atl)

Recombinant Drosophila melanogaster Atlastin (atl)

  • 中文名称：
    Recombinant Drosophila melanogaster Atlastin(atl)
  • 货号：
    CSB-CF892707DLU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    atl
  • Uniprot No.：
  • 别名：
    atl; CG6668; Atlastin
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-541
  • 氨基酸序列
    MGGSAVQVINASEEHTFVLNEDALSEVLMRDEVKDRFVCVVSVAGAFRKGKSFLLDFFLR YMYSKYVHHDATDWLGGESDPLEGFSWRGGSERDTTGILMWSDIFLHDYPNGDKIAIILL DTQGAFDSQSTVRDCATVFALSTMLSSVQIYNLSQNIQEDDLQHLQLFTEYGRLALADTG KKPFQRLQFLVRDWSFPYEAEYGALGGDKILKRRLEVSDKQHPELQSLRRHISSCFTEVA CFLMPHPGLNVATNPKFDGRLQDITPEFKSSLRSLVPMLLAPDNLVYKEISGQRVRARDL IQYFQSYMNIYKGNELPEPKSMLVATAEANHLTAVAAAKELYGQLMEEVCGGTRPYLSTA HLQTEHLRVKDKALFQFAAKRKMGGEEFTEKFRKQLEDDLEEVFTNYQAHNESKNIFKAA RTPAVYFACAVIMYILSGIFGLVGLYTFANFCNLVMGVALLTLALWAYIRYSGELSDFGG KLDDFATLLWEKFMRPIYHGCMEKGIHHVATHATEMAVGGGAASYRSQTSVNASNGKVKR S
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate microtubule polymerization and Golgi biogenesis. Required for dopaminergic neurons survival and the growth of muscles and synapses at neuromuscular junctions.
  • 基因功能参考文献：
    1. Trafficking defects produced by Atlastin dysfunction in motor neurons result in redistribution of presynaptic components and aberrant mobilization of synaptic vesicles. PMID: 28860117
    2. this paper shows that atl is required in motor neurons to affect transmitter release PMID: 26906425
    3. purified and reconstituted human ATL1 exhibited no in vitro fusion activity. When the cytosolic segment of human ATL1 was connected to the transmembrane (TM) region and C-terminal tail (CT) of Drosophila ATL PMID: 25407413
    4. Data indicate that expression of either the K320E or E328R atlastin GTPase (D-ATL) variant led to dominant-negative perturbations of the endoplasmic reticulum. PMID: 25253720
    5. Data indicate that GTP hydrolysis is required for atlastin (ATL)-mediated vesicle tethering. PMID: 25825753
    6. These data suggest that the C-terminal tail of Atlastin locally destabilizes bilayers to facilitate membrane fusion. PMID: 25555915
    7. The homotypic fusion of endoplasmic reticulum membranes is mediated by atlastin through lipid interaction with the C terminus and association of the transmembrane segments. PMID: 22802620
    8. increasing the distance of atlastin complex formation from the membrane inhibits fusion, suggesting that this distance is crucial for atlastin to promote fusion PMID: 21930898
    9. atlastin dimerizes in the presence of GTPgammaS but is monomeric with GDP or without nucleotide. Oligomerization requires the juxtamembrane middle domain three-helix bundle, as does efficient GTPase activity. PMID: 21690399
    10. Loss of spastic paraplegia gene atlastin induces age-dependent death of dopaminergic neurons in Drosophila PMID: 17030474
    11. results suggest that Atlastin controls synapse development and ER and Golgi morphogenesis by regulating microtubule stability PMID: 19341724
    12. results demonstrate that Atlastin mediates membrane tethering and fusion and strongly suggest that it is the GTPase activity that is required for endoplasmic reticulum homotypic fusion PMID: 19633650

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Colocalizes with microtubules (PubMed:19341724).
  • 蛋白家族：
    TRAFAC class dynamin-like GTPase superfamily, GB1/RHD3-type GTPase family, GB1 subfamily
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    KEGG: dme:Dmel_CG6668

    STRING: 7227.FBpp0084036

    UniGene: Dm.31277