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  4. Recombinant Arabidopsis thaliana Potassium channel KAT1 (KAT1)

Recombinant Arabidopsis thaliana Potassium channel KAT1 (KAT1)

  • 中文名称：
    拟南芥KAT1重组蛋白
  • 货号：
    CSB-CF657880DOA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    KAT1
  • Uniprot No.：
  • 别名：
    KAT1; At5g46240; MPL12.2; Potassium channel KAT1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-677
  • 氨基酸序列
    MSISWTRNFFERFCVEEYNIDTIKQSSFLSADLLPSLGARINQSTKLRKHIISPFNPRYR AWEMWLVLLVIYSAWICPFQFAFITYKKDAIFIIDNIVNGFFAIDIILTFFVAYLDSHSY LLVDSPKKIAIRYLSTWFAFDVCSTAPFQPLSLLFNYNGSELGFRILSMLRLWRLRRVSS LFARLEKDIRFNYFWIRCTKLISVTLFAIHCAGCFNYLIADRYPNPRKTWIGAVYPNFKE ASLWNRYVTALYWSITTLTTTGYGDFHAENPREMLFDIFFMMFNLGLTAYLIGNMTNLVV HWTSRTRTFRDSVRAASEFASRNQLPHDIQDQMLSHICLKFKTEGLKQQETLNNLPKAIR SSIANYLFFPIVHNIYLFQGVSRNFLFQLVSDIDAEYFPPKEDIILQNEAPTDLYILVSG AVDFTVYVDGHDQFQGKAVIGETFGEVGVLYYRPQPFTVRTTELSQILRISRTSLMSAMH AHADDGRVIMNNLFMKLRGQQSIAIDDSNTSGHENRDFKSMGWEEWRDSRKDGYGLDVTN PTSDTALMDAIHKEDTEMVKKILKEQKIERAKVERSSSETAGRSYANDSSKKDPYCSSSN QIIKPCKREEKRVTIHMMSESKNGKLILLPSSIEELLRLASEKFGGCNFTKITNADNAEI DDLDVIWDGDHLYFSSN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Highly selective inward-rectifying potassium channel. This voltage-gated channel could mediate long-term potassium influx into guard cells leading to stomatal opening. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by hyperpolarization. The channel activity is enhanced upon external acidification. Also permeable to ammonium ions. Blocked by tetraethylammonium and barium ions.
  • 基因功能参考文献：
    1. Data show that in KAT-type K(+) channel KAT1, residue K85 within the linker forms a hydrogen bond with C211 that enables the pH activation. PMID: 26846460
    2. Plant stress metabolite acrolein inhibits light-induced stomatal opening through inhibition of KAT1 potassium channels in Arabidopsis thaliana. PMID: 25144495
    3. Mutations of the central Phe residue favored the closed KAT1 channel, whereas mutations affecting the countercharge centers favored the open channel PMID: 25185120
    4. instead of single key amino acids, several titratable residues contribute to the pH-sensor of the plant Kin channel KAT1 PMID: 22070190
    5. trafficking of KAT1 has been shown to depend on ER export signals PMID: 20712625
    6. Results indicate that, in plant cells, a kinase analogous to protein kinase C might exist that may modulate KAT1 channel activity through calcium-dependent phosphorylation at some of the pinpointed residues in the cytosolic region of KAT1. PMID: 20423459
    7. Phosphatidylinositol phosphates were identified as essential regulators for the voltage-dependent and -independent activation of A. thaliana potassium channels expressed in X. laevis oocytes. [KAT1] PMID: 15842627
    8. KAT1 was expressed in mammalian HEK293 cells and measured with patch clamp technology in the whole cell configuration. The data show that a depletion of extracellular potassium results in a decrease in channel conductance. PMID: 16263909
    9. This study demonstrated trafficking of plant plasma membrane ion channels is controlled via a conserved mechanism. PMID: 16950859
    10. Structure prediction for the down state of the Kat1 voltage sensor PMID: 17187053
    11. Study reports that the hormone abscisic acid, which controls ion transport and transpiration in plants under water stress, triggers the selective endocytosis of the KAT1 K+ channel protein in epidermal and guard cells. PMID: 17683934
    12. The combination use of the selected detergents enabled the effective purification of functional KAT1 with affinity and gel-filtration chromatography. PMID: 18638455

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Potassium channel family, Plant (TC 1.A.1.4) subfamily
  • 组织特异性：
    Expressed in guard cells, and in roots.
  • 数据库链接：

    KEGG: ath:AT5G46240

    STRING: 3702.AT5G46240.1

    UniGene: At.305