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  4. Recombinant Xenopus laevis Cyclin-dependent kinase 1-A (cdk1-a)

Recombinant Xenopus laevis Cyclin-dependent kinase 1-A (cdk1-a)

  • 货号：
    CSB-YP336053XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP336053XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP336053XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP336053XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP336053XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cdk1-a
  • Uniprot No.：
  • 别名：
    cdk1-a; cdc2-a; cdc2x1.1Cyclin-dependent kinase 1-A; CDK1-A; EC 2.7.11.22; EC 2.7.11.23; Cell division control protein 2 homolog 1; Cell division control protein 2-A; Cell division protein kinase 1-A; p34 protein kinase 1
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-302
  • 氨基酸序列
    MDEYTKIEKI GEGTYGVVYK GRHKATGQVV AMKKIRLENE EEGVPSTAIR EISLLKELQH PNIVCLLDVL MQDSRLYLIF EFLSMDLKKY LDSIPSGQYI DTMLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDSKG VIKLADFGLA RAFGIPVRVY THEVVTLWYR APEVLLGSVR YSTPVDVWSI GTIFAEIATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNS FPKWKGGSLS ANVKNIDKDG LDLLAKMLIY DPAKRISARK ALLHPYFDDL DKSSLPDNQI RN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. May play a role in regulating the amplitude of the cyclic expression of circadian clock genes.
  • 基因功能参考文献：
    1. the fine-tuning of Cdc6 accumulation is essential to ensure two meiotic waves of Cdk1 activation and to avoid unscheduled DNA replication during meiotic maturation. PMID: 26092930
    2. equilibrium between CDK1 and PP2A specifies the timing of M-phase entry and exit and regulates the dynamics of cyclin B degradation upon M-phase exit in Xenopus laevis first embryonic mitosis. PMID: 26154318
    3. CDK1 activation proceeds with concomitant inhibition by CDC6, which tunes the timing of the M-phase entry during the embryonic cell cycle PMID: 25264619
    4. Xenopus Cdk1-AS rescues HT2-19 cells from apoptosis. PMID: 23525902
    5. Ras suppresses cyclin-dependent kinase 1 in a complex manner: It induces continuous accumulation of cyclin B2, but also causes persistent inhibitory phosphorylation of tyr-15-cyclin-dependent kinase 1. PMID: 23376039
    6. By promoting CtIP-dependent resection of double-strand break (DSB) ends while preventing Rad51 chromatin assembly, Cdk1 inhibits both the nonhomologous and homologous modes of DSB repair during mitosis. PMID: 21893598
    7. Examination of H1 histones reveals isoform-specific regulation by Cdk1 and RanGTP; mitotic Cdk1 functions to enhance H1 binding in egg extracts and embryos PMID: 20471264
    8. Cdc2 displays cytoskeleton-dependent localization in blastomere cortex during Xenopus embryonic cell cycle. PMID: 16097011
    9. Results suggest that the specific synthesis of either B-type cyclins or c-Mos, induced by progesterone, is required to induce meiotic maturation and Cdc2 activation. PMID: 16374506
    10. Cdk1/cyclin B has a role in regulating B-raf activation at mitosis PMID: 18434602

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 数据库链接：

    KEGG: xla:380246

    UniGene: Xl.8917