Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Vibrio vulnificus Cytolysin (vvhA)

Recombinant Vibrio vulnificus Cytolysin (vvhA)

In Stock
  • 中文名称：
    一种嗜盐弧菌vvhA重组蛋白
  • 货号：
    CSB-EP323085VFI
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    vvhA
  • Uniprot No.：
  • 别名：
    vvhA; VV2_0404; Cytolysin
  • 种属：
    Vibrio vulnificus (strain CMCP6)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    55.8 kDa
  • 表达区域：
    21-471aa
  • 氨基酸序列
    QEYVPIVEKPIYITSSKIKCVLHTSGDFNATRDWCNAGASIDVRVNVAQMRSVQSATSDGFTPDAKIVRFTVDADKPGTGIHLVNELQQDHSWFQSWANRRTYIGPFASSYDLWVKPVSGYTPKKARDLPQNENKNYQHRDTYGYSIGINGKVGAEVNKDGPKVGGEVSGSFTYNYSKTLVFDTKDYRINNRSSLSDFDISFEREFGECDELRRQELGCYFTAAHWGSGWVFDKTKFNPISYSNFKPNYDVLYEAPVSETGVTDFEMGVKLNYRARFGTVLPSALFSVYGSAGSSTNSSTVKQRIRIDWNHPLFEAEAHVTLQSLSNNDLCLDVYGENGDKTVAGGSVNGWSCHGSWNQVWGLDKEERYRSRVASDRCLTVNADKTLTVEQCGANLAQKWYWEGDKLISRYVDGNNTRYLLNIVGGRNVQVTPENEANQARWKPTLQQVKL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The synthesis of Recombinant V. vulnificus vvhA protein began with the isolation of vvhA gene from the native genome. The next step was to amplify the vvhA gene by inserting the vvhA gene into an expression vector and then introducing the vector into the selected host cell, E.coli. Significant amounts of recombinant V. vulnificus vvhA protein were produced by the host only when expression genes were added. For the purpose of protein purification, the vector contains N-terminal 10xHis tag & C-terminal Myc tag to the vvhA DNA sequence. The purity of this protein is 85%+ determined by SDS-PAGE.

    vvhA (also known as VV2_0404) is a gene encoding a protein named cytolysin in Vibrio vulnificus. The protein encoded by this gene belongs to HlyA hemolysin family. It has been proposed as a virulence factor with a molecular weight of 51kDa. This protein is specifically toxic to individual cells. It is involved in hemolysis in other organism, cytolysis and virulence.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture by mechanisms not clearly defined.
  • 蛋白家族：
    HlyA hemolysin family
  • 数据库链接：

    KEGG: vvu:VV2_0404