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  4. Recombinant Vaccinia virus Interferon antagonist K1L (VACWR032)

Recombinant Vaccinia virus Interferon antagonist K1L (VACWR032)

  • 货号：
    CSB-YP356232VAI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356232VAI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP356232VAI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356232VAI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356232VAI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VACWR032
  • Uniprot No.：
  • 别名：
    VACWR032; K1L; Interferon antagonist K1L; 32.5 kDa protein; Host range protein 1
  • 种属：
    Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-284
  • 氨基酸序列
    MDLSRINTWK SKQLKSFLSS KDAFKADVHG HSALYYAIAD NNVRLVCTLL NAGALKNLLE NEFPLHQAAT LEDTKIVKIL LFSGLDDSQF DDKGNTALYY AVDSGNMQTV KLFVKKNWRL MFYGKTGWKT SFYHAVMLND VSIVSYFLSE IPSTFDLAIL LSCIHITIKN GHVDMMILLL DYMTSTNTNN SLLFIPDIKL AIDNKDIEML QALFKYDINI YSANLENVLL DDAEIAKMII EKHVEYKSDS YTKDLDIVKN NKLDEIISKN KELRLMYVNC VKKN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibits antiviral activity induced by type I interferons. Does not block signal transduction of IFN, but is important to counter the host antiviral state induced by a pre-treatment with IFN. Plays a role in the inhibition of host NF-kappa-B activation by preventing the acetylation of the RELA/p65 subunit of NF-kappaB.
  • 基因功能参考文献：
    1. Vaccinia virus K1 inhibited acetylation of the RelA (p65) subunit of NF-kappaB, a nuclear event known to be required for NF-kappaB activation. PMID: 27503790
    2. Thus, vDeltaK1L is the first vaccinia virus construct reported that caused a muted innate immune gene expression profile and decreased immune cell infiltration in an intradermal model of infection yet still elicited protective immunity. PMID: 28490586
    3. Insertion of the K1L gene, which restores MVA replication in RK-13 cells, was not sufficient to extend MVA growth capacity to other mammalian cells. PMID: 20627347
    4. K1 residues on a continuous convex ANK repeat surface are critical for the host range function, suggesting that K1 functions through ligand interaction and does so with a novel ANK interaction surface. PMID: 20089642
    5. Here, the s provide evidence that the vaccinia virus K1 protein, a host-range protein, possesses this function. PMID: 19744687

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  • 亚细胞定位：
    Host cytoplasm. Host nucleus.
  • 数据库链接：

    KEGG: vg:3707647