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  4. Recombinant Tomato bushy stunt virus RNA silencing suppressor p19 (ORF4)

Recombinant Tomato bushy stunt virus RNA silencing suppressor p19 (ORF4)

  • 货号：
    CSB-YP320894TGA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP320894TGA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP320894TGA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP320894TGA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP320894TGA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ORF4
  • Uniprot No.：
  • 别名：
    ORF4; RNA silencing suppressor p19; 19 kDa symptom severity modulator
  • 种属：
    Tomato bushy stunt virus (strain Cherry) (TBSV)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-172
  • 氨基酸序列
    MERAIQGNDA REQANSERWD GGSGGTTSPF KLPDESPSWT EWRLHNDETN SNQDNPLGFK ESWGFGKVVF KRYLRYDRTE ASLHRVLGSW TGDSVNYAAS RFFGFDQIGC TYSIRFRGVS ITVSGGSRTL QHLCEMAIRS KQELLQLAPI EVESNVSRGC PEGTETFEKE SE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.
  • 基因功能参考文献：
    1. Both observed variation and potential variation (where a non-synonymous change to p19 would be synonymous for the overprinted movement protein) does not significantly impact protein structure or RNA binding. PMID: 26871901
    2. Data indicate that p19 exhibits size-specific and sequence-independent binding of its small RNA ligand. PMID: 23376479
    3. The results demonstrate that transient co-transformation of plant cell suspension culture with two transformation vectors is feasible and that the use of the viral suppressor of silencing p19 significantly raises the production of the protein of interest. PMID: 20859930
    4. involved in suppression of posttranscriptional gene silencing, virus movement, and symptom induction. PMID: 15299117
    5. activity hindered by defective interfering RNA PMID: 15596838
    6. This is the first clear demonstration of a direct correlation between a novel structurally orchestrated siRNA binding of an RNAi suppressor, P19 protein, and its roles in viral pathogenesis. PMID: 16501109
    7. Substitutions on P19 that compromised siRNA binding not only affected systemic invasion and symptom development but also resulted in newly recognized effects on the integrity of virus and host material in a plant species-dependent manner. PMID: 19052093
    8. P19 suppresses a common step in RNA silencing (innate immunity) that is downstream of small RNA maturation PMID: 19122141

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  • 蛋白家族：
    Tombusviruses protein p19 family
  • 数据库链接：

    KEGG: vg:1493957