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Recombinant Synsepalum dulcificum Miraculin

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  • 中文名称：
    Recombinant Synsepalum dulcificum Miraculin
  • 货号：
    CSB-EP320179RES
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Miraculin; MIR
  • 种属：
    Synsepalum dulcificum (Miracle fruit) (Richadella dulcifica)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    24.9 kDa
  • 表达区域：
    30-220aa
  • 氨基酸序列
    DSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNKTVYF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    CUSABIO transfected the expression vector which inserted the recombinant DNA into the E.coli, cultured the cells, and then induced the transcription and translation of the cloned vector. The N-terminal 10xHis tag sequence was appended to the gene coding for the E.coli of the Synsepalum dulcificum MIR protein to form the recombinant DNA. The recombinant Synsepalum dulcificum MIR was expressed as N-terminal 10xHis-tagged fusion. The purity of the protein is greater than 85% assayed by SDS-PAGE. It has an apparent molecular weight of approximately 30 kDa.

    Miraculin is a glycoprotein with taste-modifying activity and is isolated from miracle fruit (Synsepalum dulcificum). The protein can modify human's taste and turn a sour taste to a sweet taste. Recombinant miraculin was expressed in transgenic tomato plants, and was properly processed. Miraculin accumulated in transgenic tomato plants to levels higher than those in transgenic lettuce plants. This property of miraculin is helpful for preventing lifestyle diseases in that it can be used as a low-calorie sweetener or as an additive for foods targeting diabetics, as it removes the need for sugar. The active principle of the berry is the protein miraculin, which by itself is not sweet. Unlike sweet proteins, it has the unusual property of being able to turn a sour taste into a sweet taste. Sour foods, such as lemons, limes and grapefruit, taste sweet when tasted together with this protein. Namely, Miraculin by itself is not sweet, but it is able to turn a sour taste into a sweet taste. This unique property has led to increasing interest in this protein.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Miraculin has the property of modifying a sour taste into a sweet taste. This alteration of taste perception persists for many minutes.
  • 蛋白家族：
    Protease inhibitor I3 (leguminous Kunitz-type inhibitor) family
  • 组织特异性：
    Expressed in fruit pulp after pollination. Not expressed in seeds, stems or leaves.