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  4. Recombinant Staphylococcus aureus Peptide deformylase (def)

Recombinant Staphylococcus aureus Peptide deformylase (def)

In Stock
  • 中文名称：
    Recombinant Staphylococcus aureus Peptide deformylase(def)
  • 货号：
    CSB-YP017707FKZ
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    def
  • Uniprot No.：
  • 别名：
    def; def1; pdf1Peptide deformylase; PDF; EC 3.5.1.88; Polypeptide deformylase
  • 种属：
    Staphylococcus aureus
  • 蛋白长度：
    Full Length
  • 来源：
    Yeast
  • 分子量：
    22.6kDa
  • 表达区域：
    1-183aa
  • 氨基酸序列
    MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNSQDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQEAYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLNGVMFYDHIDKNHPLQPHTDAVEV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have purchased your E. coli expressed def recombinant protein (cat#: CSB-EP017707FKZ; Lot#: 03380).I would like to develop a stable recombinant peptide deformylase. The half-life of this enzyme is really short (10 seconds) and I want to improve it.Do you have any information on how to increase the half-life?

A:
Very nice to receive your inquiry. We're sorry but we haven't performed related experiment to study the half-life of this protein and haven't received any relevant feedback from other customers.
You has mentioned that "The half-life of this enzyme is really short (10 seconds)". What analysis did you do? How did you get this conclusion?
Here we provide a literature for your reference. Here is the link: http://europepmc.org/articles/PMC2312423

靶点详情

  • 功能：
    Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.
  • 蛋白家族：
    Polypeptide deformylase family