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  4. Recombinant Schizosaccharomyces pombe Poly (A) RNA polymerase protein cid1

Recombinant Schizosaccharomyces pombe Poly (A) RNA polymerase protein cid1

  • 中文名称：
    Recombinant Schizosaccharomyces pombe Poly(A) RNA polymerase protein cid1,Yeast
  • 货号：
    CSB-YP515592SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Poly(A) RNA polymerase protein cid1,Yeast
  • 货号：
    CSB-EP515592SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Poly(A) RNA polymerase protein cid1,Yeast
  • 货号：
    CSB-EP515592SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Poly(A) RNA polymerase protein cid1,Yeast
  • 货号：
    CSB-BP515592SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Poly(A) RNA polymerase protein cid1,Yeast
  • 货号：
    CSB-MP515592SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cid1
  • Uniprot No.：
  • 别名：
    cid1; SPAC19D5.03Terminal uridylyltransferase cid1; TUTase cid1; EC 2.7.7.19; EC 2.7.7.52; Caffeine-induced death protein 1; Poly(A) polymerase cid1; PAP; Poly(U) polymerase cid1; PUP
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-405
  • 氨基酸序列
    MNISSAQFIP GVHTVEEIEA EIHKNLHISK SCSYQKVPNS HKEFTKFCYE VYNEIKISDK EFKEKRAALD TLRLCLKRIS PDAELVAFGS LESGLALKNS DMDLCVLMDS RVQSDTIALQ FYEELIAEGF EGKFLQRARI PIIKLTSDTK NGFGASFQCD IGFNNRLAIH NTLLLSSYTK LDARLKPMVL LVKHWAKRKQ INSPYFGTLS SYGYVLMVLY YLIHVIKPPV FPNLLLSPLK QEKIVDGFDV GFDDKLEDIP PSQNYSSLGS LLHGFFRFYA YKFEPREKVV TFRRPDGYLT KQEKGWTSAT EHTGSADQII KDRYILAIED PFEISHNVGR TVSSSGLYRI RGEFMAASRL LNSRSYPIPY DSLFEEAPIP PRRQKKTDEQ SNKKLLNETD GDNSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cytoplasmic uridylyltransferase that mediates the terminal uridylation of mRNAs with short poly(A) tails such as such as act1, hcn1 and urg1 mRNAs, hence facilitating global mRNA decay. Uridylates the 3' ends of actin mRNAs upon S-phase arrest. Has also a weak poly(A) polymerase (PAP) activity. Residue His-336 is responsible for the specificity for UTP. Involved in cell cycle arrest where in association with crb2/rhp9 and chk1 it inhibits unscheduled mitosis.
  • 基因功能参考文献：
    1. Data show that a hinge region (N164-N165) of terminal uridylyltransferase Cid1 is essential for catalytic activity. PMID: 25712096
    2. Structural analysis of Cid1 bound to a non-hydrolyzable nucleotide UMPNPP and bound to its minimal pseudo-product ApU revealed new key residues for the substrate/product recognition. PMID: 24322298
    3. This work demonstrates that Cid1 is able to accommodate all 4 tested ribonucleotide triphosphates (NTP) in the active site, but there are differences in recognition that suggest UTP is the preferred NTP. PMID: 22885303
    4. describe crystal structures of cytoplasmic terminal uridylyl transferase Cid1 in apo conformers and bound to UTP, and demonstrate that a single histidine residue, conserved in mammalian Cid1 orthologs, is responsible for discrimination between UTP and ATP PMID: 22751018
    5. Cid1 RNA binding properties, a feature with critical implications for miRNAs, histone mRNAs, and, more generally, cellular RNA degradation PMID: 22608966
    6. Results describe the biochemical activity of fission yeast Schizosaccharomyces pombe Cid1, a putative cytoplasmic poly(A) polymerase implicated in cell cycle checkpoint controls. PMID: 17353264
    7. The Schizosaccharomyces pombe cytoplasmic protein Cid1 acts as a poly(U) polymerase (PUP). Polyadenylated actin mRNA, a target of this activity, is uridylated upon arrest in S phase and is likely to be one of many such Cid1 targets PMID: 18371314

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    DNA polymerase type-B-like family
  • 数据库链接：

    KEGG: spo:SPAC19D5.03

    STRING: 4896.SPAC19D5.03.1