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  4. Recombinant Schizosaccharomyces pombe Mating-type switching protein swi5 (swi5)

Recombinant Schizosaccharomyces pombe Mating-type switching protein swi5 (swi5)

  • 中文名称：
    庞贝裂殖酵母swi5重组蛋白
  • 货号：
    CSB-YP892061SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母swi5重组蛋白
  • 货号：
    CSB-EP892061SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母swi5重组蛋白
  • 货号：
    CSB-EP892061SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母swi5重组蛋白
  • 货号：
    CSB-BP892061SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母swi5重组蛋白
  • 货号：
    CSB-MP892061SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    swi5
  • Uniprot No.：
  • 别名：
    swi5; SPBC409.03; Mating-type switching protein swi5; DNA repair protein swi5
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-85
  • 氨基酸序列
    MEKSQLESRV HLLEQQKEQL ESSLQDALAK LKNRDAKQTV QKHIDLLHTY NEIRDIALGM IGKVAEHEKC TSVELFDRFG VNGSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for normal mating-type switching. Also involved in the rhp51-dependent recombination DNA repair pathway.
  • 基因功能参考文献：
    1. s propose that the role of a coplanar alignment of nucleobases induced by Swi5-Sfr1 in the presynaptic Rad51/ssDNA complex is to facilitate the critical matching with an invading double-stranded DNA, hence stimulating the strand exchange reaction. PMID: 24304898
    2. Characterized is the intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering. PMID: 23324799
    3. s propose a model placing Rrp1 and Rrp2 functioning together with Swi5 and Srs2 in a synthesis-dependent strand annealing homologous recombination repair pathway. PMID: 23828040
    4. Data propose a model for how the Swi5-Sfr1 complex binds to the Rad51 filament, in which the Swi5-Sfr1 complex fits into the groove of the Rad51 filament, leading to an active and stable presynaptic filament. PMID: 22033972
    5. Schizosaccharomyces pombe Swi5-Sfr1 complex, a conserved eukaryotic protein complex, at substoichiometric concentrations stimulates strand exchange mediated by Rhp51 (the S. pombe Rad51 homolog) and Dmc1 on long DNA substrates. PMID: 16921379
    6. a new role for Swi5 and the histone acetylation status in mat-bias establishment through directionality of repair from the intact chromatid to the broken chromatid. PMID: 18845847

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  • 蛋白家族：
    SWI5/SAE3 family
  • 数据库链接：

    KEGG: spo:SPBC409.03

    STRING: 4896.SPBC409.03.1